Date published: 2025-9-6
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Skeletal Muscle Myosin Antibody (F59) - Western Blotting - Image 9119
骨骼肌肌球蛋白抗体 (F59): sc-32732. 小鼠心脏 (A) 和大鼠心脏 (B) 组织提取物中骨骼肌肌球蛋白表达的 Western 印迹分析.
骨骼肌肌球蛋白抗体 (F59): sc-32732. 小鼠心脏 (A) 和大鼠心脏 (B) 组织提取物中骨骼肌肌球蛋白表达的 Western 印迹分析.

Skeletal Muscle Myosin 抗体 (F59): sc-32732

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说明书
  • Skeletal Muscle Myosin 抗体 F59 是小鼠单克隆 IgG1 κ，Skeletal Muscle Myosin抗体, 在68篇文献中引用，规格为200 µg/ml
  • 针对从成人胸肌中纯化的肌球蛋白的抗体，white Leghorn chicken来源
  • Skeletal Muscle Myosin 抗体 (F59) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat, human 和avian 来源的 fast fiber–specific skeletal muscle myosin heavy chain
  • 抗Skeletal Muscle Myosin抗体（F59）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Skeletal Muscle Myosin (F59): sc-32732的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 Skeletal Muscle Myosin 抗体 (F59) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Skeletal Muscle Myosin 抗体 (F59) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Myosin 抗体包括 MRLC2, MYH, MYH1, MYH10, MYH11, MYH15, MYH2, MYH3, MYH4 和 MYH6

骨骼肌肌球蛋白抗体（F59）是一种小鼠单克隆IgG1 kappa轻链抗体，用于识别快速纤维特异性骨骼肌肌球蛋白重链，包括MYH1、MYH2、MYH4和MYH6，适用于多种物种，如小鼠、大鼠、人类和鸟类。抗骨骼肌肌球蛋白抗体（F59）是针对从成年白莱鸡胸肌中纯化的肌球蛋白制备的，可用于免疫印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学等应用。骨骼肌肌球蛋白是一种高度保守且普遍表达的运动蛋白，通过与肌动蛋白相互作用产生细胞运动所需的力，在肌肉收缩中起着关键作用。作为由MYH基因家族编码的肌球蛋白重链家族的关键成员，MYH2具有肌动蛋白激活的ATP酶活性，这是其运动功能和调节肌肉收缩速度所必需的。 了解并检测MYH2对于研究肌肉生理学、疾病机制和发育生物学至关重要，因为不同的肌球蛋白同工型根据肌肉功能活动而具有不同的表达。骨骼肌肌球蛋白（F59）单克隆抗体有非结合型和多种结合型，包括辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®变体，可满足各种实验需求。建议研究骨骼肌功能和病理学分子动力学的科学家使用抗骨骼肌肌球蛋白抗体（F59）。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Skeletal Muscle Myosin 抗体 (F59) 参考文献:

  1. 骨骼肌发育过程中肌球蛋白重链基因表达的时空变化  |  Lu, BD., et al. 1999. Dev Biol. 216: 312-26. PMID: 10588881
  2. 肌球蛋白同工型、肌纤维类型和转换。  |  Pette, D. and Staron, RS. 2000. Microsc Res Tech. 50: 500-9. PMID: 10998639
  3. 鉴定人类肌球蛋白重链的三种发育受控异构体  |  Bober, E., et al. 1990. Eur J Biochem. 189: 55-65. PMID: 1691980
  4. 平滑肌肌球蛋白由同二聚体重链组成。  |  Kelley, CA., et al. 1992. J Biol Chem. 267: 2127-30. PMID: 1733920
  5. 生成全长的人类围产期肌球蛋白重链编码 cDNA。  |  Karsch-Mizrachi, I., et al. 1990. Gene. 89: 289-94. PMID: 2373371
  6. 肌球蛋白磷酸化对骨骼肌收缩的调节。  |  Vandenboom, R. 2016. Compr Physiol. 7: 171-212. PMID: 28135003
  7. 体育锻炼会影响骨骼肌肌球蛋白的静态构象，降低其ATP消耗。  |  Lewis, CTA., et al. 2023. J Gen Physiol. 155: PMID: 37227464
  8. 人类围产期肌球蛋白重链转录本的特征。  |  Jullian, EH., et al. 1995. Eur J Biochem. 230: 1001-6. PMID: 7601129
  9. 调节血管平滑肌细胞的分化。  |  Owens, GK. 1995. Physiol Rev. 75: 487-517. PMID: 7624392
  10. 肌球蛋白超家族的系统发育分析。  |  Cheney, RE., et al. 1993. Cell Motil Cytoskeleton. 24: 215-23. PMID: 8477454
  11. 平滑肌收缩机制  |  Horowitz, A., et al. 1996. Physiol Rev. 76: 967-1003. PMID: 8874491
  12. 哺乳动物肌球蛋白重链基因家族。  |  Weiss, A. and Leinwand, LA. 1996. Annu Rev Cell Dev Biol. 12: 417-39. PMID: 8970733

订购信息

产品名称产品编号规格价格数量收藏夹

Skeletal Muscle Myosin 抗体 (F59)

sc-32732
200 µg/ml
$316.00

Skeletal Muscle Myosin (F59): m-IgG Fc BP-HRP 套装

sc-528466
200 µg Ab; 10 µg BP
$354.00

Skeletal Muscle Myosin (F59): m-IgGκ BP-HRP 套装

sc-520852
200 µg Ab, 40 µg BP
$354.00

Skeletal Muscle Myosin (F59): m-IgG1 BP-HRP 套装

sc-542945
200 µg Ab; 20 µg BP
$354.00

Skeletal Muscle Myosin 抗体 (F59) AC

sc-32732 AC
500 µg/ml, 25% agarose
$416.00

Skeletal Muscle Myosin 抗体 (F59) HRP

sc-32732 HRP
200 µg/ml
$316.00

Skeletal Muscle Myosin 抗体 (F59) FITC

sc-32732 FITC
200 µg/ml
$330.00

Skeletal Muscle Myosin 抗体 (F59) PE

sc-32732 PE
200 µg/ml
$343.00

Skeletal Muscle Myosin 抗体 (F59) Alexa Fluor® 488

sc-32732 AF488
200 µg/ml
$357.00

Skeletal Muscle Myosin 抗体 (F59) Alexa Fluor® 546

sc-32732 AF546
200 µg/ml
$357.00

Skeletal Muscle Myosin 抗体 (F59) Alexa Fluor® 594

sc-32732 AF594
200 µg/ml
$357.00

Skeletal Muscle Myosin 抗体 (F59) Alexa Fluor® 647

sc-32732 AF647
200 µg/ml
$357.00

Skeletal Muscle Myosin 抗体 (F59) Alexa Fluor® 680

sc-32732 AF680
200 µg/ml
$357.00

Skeletal Muscle Myosin 抗体 (F59) Alexa Fluor® 790

sc-32732 AF790
200 µg/ml
$357.00

Does this antibody detect any specific myosin heavy chain? Such as MYH1, MYH6, MYH7, MYH15, MYH2, MYH3, MYH4 etc?

Asked by: Eric Spinetti
Thank you for your question. The exact epitope recognized by this mouse monoclonal antibody has not yet been determined. This clone is recommended for the detection of fast fiber–specific skeletal muscle myosin heavy chain. The exact myosin heavy chain genes/proteins that are targeted are not known.
Answered by: Technical Support
Date published: 2022-08-12

I am interested in performing a immunoprecipitation of MYH6 using this antibody. However, I don't see any validation of this technique in your website or in any cited papers. Do you have any data demonstrating the efficiency of F59 (sc-32732) for IP?

Asked by: Anonymous
This primary antibody is recommended and warrantied for immunoprecipitation (IP), however we are still gathering data to show on our website. Check back for updates!
Answered by: Tech Service
Date published: 2019-09-20

I need to use an antibody to detect the protein in samples of canine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 15
Date published: 2017-01-11

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 15
Date published: 2017-01-11
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Rated 4 out of 5 by from Good signalThis antibody works well on human and mouse samples in WB
Date published: 2018-08-01
Rated 4 out of 5 by from Great features!The antibody is work well in WB at 1:100 of C2C12 cells.
Date published: 2017-08-31
Rated 5 out of 5 by from goodWB dilution 1/1000, mouse heart tissue detected clean band
Date published: 2017-05-09
Rated 5 out of 5 by from Sehr gutApplication in mouse cells, very clear immunolabelling.
Date published: 2017-04-05
Rated 5 out of 5 by from OutstandingStrong western blot data, low background; nice IHC staining
Date published: 2017-01-29
Rated 5 out of 5 by from Positive result in WBI bought it for years，tested it in WB with pig dorsal muscles，it is very wonderful
Date published: 2016-12-13
Rated 5 out of 5 by from Western blot and IF publications using mouseWestern blot and IF publications using mouse C2C12 myoblasts. -SCBT Publication Review
Date published: 2015-03-12
Rated 5 out of 5 by from Publishable IHC data in mouse cardiomyocytesPublishable IHC data in mouse cardiomyocytes. -SCBT Publication Review
Date published: 2015-02-08
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Skeletal Muscle Myosin 抗体 (F59) is rated 4.8 out of 5 by 10.
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