Date published: 2025-9-7
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SGLT-2 Antibody (D-6) - Western Blotting - Image 393006
SGLT-2 抗体 (D-6) HRP: sc-393350 HRP. Jurkat (A), K-562 (B), Caki-1 (C), c4 (D) 和 EOC 20 (E) 全细胞裂解液中 SGLT-2 表达的直接 Western 印迹分析.
SGLT-2 抗体 (D-6) HRP: sc-393350 HRP. Jurkat (A), K-562 (B), Caki-1 (C), c4 (D) 和 EOC 20 (E) 全细胞裂解液中 SGLT-2 表达的直接 Western 印迹分析.

SGLT-2 抗体 (D-6): sc-393350

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说明书
  • SGLT-2 抗体 D-6 是小鼠单克隆 IgG1 κ，SGLT-2抗体, 在29篇文献中引用，规格为200 µg/ml
  • 与human的SGLT-2的细胞外结构域内的氨基酸226-268杂交
  • SGLT-2 抗体 (D-6) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 SGLT-2
  • 抗SGLT-2抗体（D-6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取SGLT-2 (D-6): sc-393350的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 SGLT-2 抗体 (D-6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与SGLT-2 抗体 (D-6) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

SGLT 抗体

SGLT-2抗体（D-6）是一种IgG1 κ型小鼠单克隆SGLT-2抗体（也被命名为SC5A2抗体、溶质载体家族5成员2抗体、溶质载体家族5（钠/葡萄糖共转运体）成员2抗体、钠/葡萄糖共转运体2抗体、Na（+）/葡萄糖共转运体2抗体、SGLT2抗体或溶质载体家族5（钠/葡萄糖转运体）成员2抗体），它能够通过蛋白质印迹法、免疫沉淀法、免疫荧光法、免疫组织化学（石蜡）和酶联免疫吸附法检测小鼠、大鼠和人类来源的SGLT-2蛋白。SGLT-2抗体（D-6）既有非偶联形式的抗SGLT-2抗体，也有多种偶联形式的抗SGLT-2抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。葡萄糖是哺乳动物细胞的主要能量来源，其进入细胞的过程由各种转运体介导。这一过程涉及七个促进性（GLUT-1至-7）和两个浓缩性葡萄糖转运体（SGLT-1，SGLT-2）以及一个传感器（SGLT-3）。SGLT家族成员利用两个钠离子的电化学梯度来转运一个葡萄糖分子。SGLTs的mRNA从胎儿期到成熟期随着其功能活性的增加而稳步增加。SGLT-1负责从肠腔中摄取饮食中的葡萄糖和半乳糖，而SGLT-3仅参与肠腔葡萄糖的检测。钠葡萄糖共转运体SGLT-1和SGLT-2均在肾脏中表达。编码SGLT-2的基因发生突变会导致家族性肾性糖尿（FRG），这是一种近端肾小管葡萄糖转运的孤立性障碍，其特征是在正常血糖水平下出现异常的尿糖排泄。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

SGLT-2 抗体 (D-6) 参考文献:

  1. 钠葡萄糖共转运体-1 在哺乳动物肾小管生成过程中的表达特征和相关性。  |  Yang, Q., et al. 2000. Am J Physiol Renal Physiol. 279: F765-77. PMID: 10997927
  2. 葡萄糖转运体在哺乳动物肾脏和肾脏发育中的地位。  |  Wallner, EI., et al. 2001. Ren Fail. 23: 301-10. PMID: 11499546
  3. 缺乏 GLUT2 时肠内葡萄糖吸收的正常动力学：需要葡萄糖磷酸化并转运到内质网的转运途径的证据。  |  Stümpel, F., et al. 2001. Proc Natl Acad Sci U S A. 98: 11330-5. PMID: 11562503
  4. 葡萄糖转运体家族 SGLT 和 GLUT：正常和异常功能的分子基础。  |  Scheepers, A., et al. 2004. JPEN J Parenter Enteral Nutr. 28: 364-71. PMID: 15449578
  5. 一名常染色体隐性肾性葡萄糖尿症患者的新型 SGLT2 基因突变。  |  Francis, J., et al. 2004. Nephrol Dial Transplant. 19: 2893-5. PMID: 15496564
  6. SLC5A2 编码 SGLT2 的新型错义突变是常染色体隐性肾性葡萄糖尿症和氨基酸尿症的基础。  |  Magen, D., et al. 2005. Kidney Int. 67: 34-41. PMID: 15610225
  7. 从非胰岛素依赖型糖尿病患者尿液中分离出的人肾近曲小管细胞中的葡萄糖转运体。  |  Rahmoune, H., et al. 2005. Diabetes. 54: 3427-34. PMID: 16306358
  8. 大鼠肠腔葡萄糖感应具有钠-葡萄糖共转运体的特征。  |  Freeman, SL., et al. 2006. Am J Physiol Gastrointest Liver Physiol. 291: G439-45. PMID: 16675747
  9. SGLT-2抑制剂与感染风险：随机对照试验的系统评价和荟萃分析。  |  Puckrin, R., et al. 2018. Acta Diabetol. 55: 503-514. PMID: 29484489
  10. SGLT-2抑制剂在糖尿病肾病治疗中的作用和机制。  |  Dai, ZC., et al. 2023. Front Immunol. 14: 1213473. PMID: 37809091
  11. 英国肾脏协会临床实践指南：肾病患者钠-葡萄糖协同转运蛋白-2（SGLT-2）抑制剂2023更新。  |  Roddick, AJ., et al. 2023. BMC Nephrol. 24: 310. PMID: 37880609
  12. 人类 Na+/葡萄糖共转运体基因 SGLT1 在染色体 22q13.1 上的定位。  |  Turk, E., et al. 1993. Genomics. 17: 752-4. PMID: 8244393

订购信息

产品名称产品编号规格价格数量收藏夹

SGLT-2 抗体 (D-6)

sc-393350
200 µg/ml
$316.00

SGLT-2 (D-6): m-IgG Fc BP-HRP 套装

sc-530435
200 µg Ab; 10 µg BP
$354.00

SGLT-2 (D-6): m-IgGκ BP-HRP 套装

sc-523913
200 µg Ab, 40 µg BP
$354.00

SGLT-2 (D-6): m-IgG1 BP-HRP 套装

sc-544050
200 µg Ab; 20 µg BP
$354.00

SGLT-2 抗体 (D-6) AC

sc-393350 AC
500 µg/ml, 25% agarose
$416.00

SGLT-2 抗体 (D-6) HRP

sc-393350 HRP
200 µg/ml
$316.00

SGLT-2 抗体 (D-6) FITC

sc-393350 FITC
200 µg/ml
$330.00

SGLT-2 抗体 (D-6) PE

sc-393350 PE
200 µg/ml
$343.00

SGLT-2 抗体 (D-6) Alexa Fluor® 488

sc-393350 AF488
200 µg/ml
$357.00

SGLT-2 抗体 (D-6) Alexa Fluor® 546

sc-393350 AF546
200 µg/ml
$357.00

SGLT-2 抗体 (D-6) Alexa Fluor® 594

sc-393350 AF594
200 µg/ml
$357.00

SGLT-2 抗体 (D-6) Alexa Fluor® 647

sc-393350 AF647
200 µg/ml
$357.00

SGLT-2 抗体 (D-6) Alexa Fluor® 680

sc-393350 AF680
200 µg/ml
$357.00

SGLT-2 抗体 (D-6) Alexa Fluor® 790

sc-393350 AF790
200 µg/ml
$357.00

貴族のSGLT2抗体(D-6)を用てFCMを検討しております。使用するアイソタイプと蛍光抗体の組み合わせのおすすめはありますでしょうか。 ご教示いただけましたら幸いです。

Asked by: zaki
Thank you for your question. Please contact our Technical Service Department for more information. Our Asian Technical Service team is asia@scbio.cn or by live chat on our website.
Answered by: Jiajun Z
Date published: 2025-04-15

Technical Support says the antibody is raised against amino acids 226-268 mapping within an internal region of SGLT-2 in Q&A. However, the data sheat says SGLT-2(D-6) is raised against aa 220-270 mapping within an extracellular region. Which is true?

Asked by: HappSie
Thanks for your question. The exact peptide sequence used to raise this antibody is proprietary. However, we can give a 50 amino acid range. Anti-SGLT-2 Antibody (D-6): sc-393350 is raised against amino acids 226-268 mapping within an extracellular domain of SGLT-2 of human origin.
Answered by: Yanru Tao
Date published: 2022-06-24

Hi Does this antibody SGLT-2 Antibody (D-6): sc-393350 detect SGLT2 isoform 2 ?

Asked by: Azam
Thank you for your question. Yes, this mouse monoclonal SGLT-2 (D-6) should detect both isoforms.
Answered by: Tech Service
Date published: 2020-08-04

Hi Does SGLT2 antibody detect rabbit SGLT2?

Asked by: Azam
Thank you for your question. We have not tested this mouse monoclonal antibody, SGLT-2 (D-6): sc-393350, for reactivity in rabbit samples. The antibody is raised against amino acids 226-268 mapping within an internal region of SGLT-2 of human origin. Based on sequence analysis, the immunogen sequence does share 91% sequence identity with the published protein sequence for rabbit SGLT2 (see link below as a reference for that sequence). This is a very high degree of sequence identity, and there is a good chance that this antibody would detect rabbit SGLT-2. http://www.uniprot.org/uniprot/P26430
Answered by: Technical Support
Date published: 2017-10-04

I was wondering if this antibody reacts with mouse SGLT-2. They share 86% identity in the amino acid sequence used as a antigen. If not, could you recommend one for IHC/WB?

Asked by: Arrosung01
Thank you for your question. We have not yet tested this mouse monoclonal, SGLT-2 (D-6): sc-393350, for detection of SGLT-2 in mouse samples. Based on the sequence identity with the antigen, it is highly likely that this antibody will also detect the mouse protein. If you would be interested in testing a free 10 µg sample of SGLT-2 (D-6): sc-393350 for this application, please contact our Technical Service Department. Please call our Asian Support office toll-free at 800-820-8626, email asia@scbio.cn, or contact us by live chat.
Answered by: Technical Support
Date published: 2017-01-25

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with sc-393350: SGLT-2 (D-6)?

Asked by: cjMara
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 7
Date published: 2020-12-17

Does sc-393350 cross-react with SGLT-1?

Asked by: cjMara
sc-393350: SGLT-2 (D-6) is a mouse monoclonal antibody that is specific for SGLT-2. It is not expected to cross-react with SGLT-1.
Answered by: Technical Support 7
Date published: 2020-08-04
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Rated 4 out of 5 by from Good antibodyWorking properly for more than 6 time after the use
Date published: 2025-02-06
Rated 5 out of 5 by from ExcellentThis antibody is suitable for the immunoblot analysis
Date published: 2024-10-16
Rated 5 out of 5 by from ottimo funzionamentol'anticorpo è molto specifico, le bande ottenute sono nitide, lo consiglio
Date published: 2021-02-02
Rated 2 out of 5 by from WB細胞lysateを使用したウエスタンブロットの実験でSGLT2の発現を確認しました。バンドを検出しました。
Date published: 2020-12-01
Rated 5 out of 5 by from tres bienI already bought and the signal was great. Effcient
Date published: 2020-06-19
Rated 4 out of 5 by from Good antibodyUsed antibody on mouse kidney tissue and very crisp!
Date published: 2018-01-04
Rated 4 out of 5 by from WB useUsed this antibody for WB of mouse kidneys and worked great! 1:1000 dilution
Date published: 2017-12-21
Rated 5 out of 5 by from Superb !!Outstanding product for IHC; also great WB results
Date published: 2017-01-18
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SGLT-2 抗体 (D-6) is rated 4.4 out of 5 by 10.
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