Date published: 2025-9-6

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SGK3 抗体 (C-6): sc-166847

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说明书
  • SGK3 抗体 C-6 是小鼠单克隆 IgG2b κ,SGK3抗体, 在8篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列67-145位于human物种的SGK3的N-terminus附近
  • SGK3 抗体 (C-6) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 SGK3
  • 抗SGK3抗体(C-6)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取SGK3 (C-6): sc-166847免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP2b BP-HRP">m-IgG2b BP-HRPm-IgGκ BP-HRP是SGK3 Antibody (C-6) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与SGK3 Antibody (C-6) 打包提供(请参阅下面的订购信息)。

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関連項目

SGK3抗体(C-6)是一种IgG2bκ型小鼠单克隆SGK3抗体(也被称为SGK3抗体),它可以通过WB、IP、IF、IHC(P)和ELISA等方法检测到小鼠、大鼠和人类来源的SGK3蛋白。SGK3抗体(C-6)可以以非共轭型抗SGK3抗体形式获得,也可以以多种共轭型抗SGK3抗体形式获得,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。丝氨酸/苏氨酸蛋白激酶Sgk3(SGK3),也被称为血清/糖皮质激素调节激酶3,属于Ser/Thr蛋白激酶家族。血清和糖皮质激素调节激酶蛋白与Akt蛋白家族密切相关。SGK3的同源蛋白SGK1能够激活离子通道,特别是钾(K+)通道。研究发现,SGK2和SGK3也参与了这一激活过程,使这三种蛋白成为细胞增殖、上皮转运和神经肌肉兴奋性的重要调节因子。SGK3在细胞中作为IL-3依赖的生存信号的介质发挥作用。它定位于早期内体和类似囊泡的结构中。SGK3是一种广泛表达的蛋白,但主要在肾脏、肝脏、胰腺、大脑和心脏中被检测到。SGK3在Ser 486位点的磷酸化会导致SGK3激活的增加。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

SGK3 抗体 (C-6) 参考文献:

  1. 血清和糖皮质激素诱导蛋白激酶两种新型异构体的结构和调控特征。  |  Kobayashi, T., et al. 1999. Biochem J. 344 Pt 1: 189-97. PMID: 10548550
  2. 克隆新型人类血清/糖皮质激素调节激酶样基因 SGKL 并将其绘制到染色体 8q12.3-q13.1 上。  |  Dai, F., et al. 1999. Genomics. 62: 95-7. PMID: 10585774
  3. 血清和糖皮质激素依赖性蛋白激酶 SGK 的所有三种同工酶激活 K+ 通道。  |  Gamper, N., et al. 2002. Pflugers Arch. 445: 60-6. PMID: 12397388
  4. 血清和糖皮质激素诱导激酶对通道的调控--对转运, 兴奋性和细胞增殖的影响。  |  Lang, F., et al. 2003. Cell Physiol Biochem. 13: 41-50. PMID: 12649601
  5. 泛素连接酶 Nedd4-2 和血清及糖皮质激素诱导激酶 SGK1 对电压门控 K+ 通道 Kv1.3 的调控。  |  Henke, G., et al. 2004. J Cell Physiol. 199: 194-9. PMID: 15040001
  6. 靶向干扰蛋白激酶 SGK3/CISK 会损害出生后的毛囊发育。  |  McCormick, JA., et al. 2004. Mol Biol Cell. 15: 4278-88. PMID: 15240817
  7. 丝氨酸/苏氨酸激酶 SGK1, 3 和 PKB 可刺激氨基酸转运体 ASCT2。  |  Palmada, M., et al. 2005. Biochem Biophys Res Commun. 331: 272-7. PMID: 15845389
  8. SGK3对转运相关疾病的贡献。  |  Liao, QQ., et al. 2022. Front Cell Dev Biol. 10: 1007924. PMID: 36531961
  9. SGK3/TOPK信号通路在急性肾损伤向慢性肾病转变中的作用。  |  Shu, H., et al. 2023. Front Pharmacol. 14: 1169054. PMID: 37361201
  10. SGK3-过氧化氢酶抗氧化信号轴驱动宫颈癌生长和耐药性。  |  Wang, M., et al. 2023. Redox Biol. 67: 102931. PMID: 37866161
  11. SGK3通过Pit-1促进慢性肾脏病血管钙化  |  Dong, QQ., et al. 2024. Theranostics. 14: 861-878. PMID: 38169564
  12. 巨噬细胞中SGK3的缺乏通过调节Ndufa13介导的线粒体氧化应激抑制血管紧张素II诱导的心脏重塑。  |  Ren, J., et al. 2024. Cell Mol Life Sci. 81: 359. PMID: 39158709

订购信息

产品名称产品编号规格价格数量收藏夹

SGK3 抗体 (C-6)

sc-166847
200 µg/ml
$316.00

SGK3 (C-6): m-IgG Fc BP-HRP 套装

sc-529070
200 µg Ab; 10 µg BP
$354.00

SGK3 (C-6): m-IgGκ BP-HRP 套装

sc-521771
200 µg Ab, 40 µg BP
$354.00

SGK3 (C-6): m-IgG2b BP-HRP 套装

sc-550076
200 µg Ab; 10 µg BP
$354.00

SGK3 抗体 (C-6) AC

sc-166847 AC
500 µg/ml, 25% agarose
$416.00

SGK3 抗体 (C-6) HRP

sc-166847 HRP
200 µg/ml
$316.00

SGK3 抗体 (C-6) FITC

sc-166847 FITC
200 µg/ml
$330.00

SGK3 抗体 (C-6) PE

sc-166847 PE
200 µg/ml
$343.00

SGK3 抗体 (C-6) Alexa Fluor® 488

sc-166847 AF488
200 µg/ml
$357.00

SGK3 抗体 (C-6) Alexa Fluor® 546

sc-166847 AF546
200 µg/ml
$357.00

SGK3 抗体 (C-6) Alexa Fluor® 594

sc-166847 AF594
200 µg/ml
$357.00

SGK3 抗体 (C-6) Alexa Fluor® 647

sc-166847 AF647
200 µg/ml
$357.00

SGK3 抗体 (C-6) Alexa Fluor® 680

sc-166847 AF680
200 µg/ml
$357.00

SGK3 抗体 (C-6) Alexa Fluor® 790

sc-166847 AF790
200 µg/ml
$357.00

I am using SGK3 (C-6): sc-166847 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Germaine
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2023-11-16
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Rated 4 out of 5 by from Good antibodyThe antibody detects a very strong band near 50-kDa and another strong band near 60-kDa which is decreased by SGK3 siRNA.
Date published: 2017-09-11
Rated 4 out of 5 by from Potent and quite specific antibodyThis monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was strong detection of the ~65+53 kDa target proteins in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested, but slightly higher backgrounds.
Date published: 2016-11-10
Rated 5 out of 5 by from Excellent immunoperoxidase cytoplasmic andExcellent immunoperoxidase cytoplasmic and nuclear staining in formalin fixed, paraffin-embedded human gall bladder tissue. -SCBT QC
Date published: 2015-03-30
Rated 5 out of 5 by from Positive western blot detection of SGK3 expressionPositive western blot detection of SGK3 expression in A-375 whole cell lysate. -SCBT QC
Date published: 2015-02-02
Rated 5 out of 5 by from Strong signal in immunofluorescence stainingStrong signal in immunofluorescence staining of methanol-fixed HeLa cells showed cytoplasmic localization. -SCBT QC
Date published: 2015-01-17
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SGK3 抗体 (C-6) is rated 4.6 out of 5 by 5.
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