Date published: 2025-9-7
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SCF Antibody (G-3) - Western Blotting - Image 356289
SCF 抗体 (G-3): sc-13126. MG-63 (A), NCI-H1299 (B), NIH/3T3 (C), Neuro-2A (D), C6 (E) 和 PC-12 (F) 全细胞裂解液中 SCF 表达的 Western 印迹分析.
SCF 抗体 (G-3): sc-13126. MG-63 (A), NCI-H1299 (B), NIH/3T3 (C), Neuro-2A (D), C6 (E) 和 PC-12 (F) 全细胞裂解液中 SCF 表达的 Western 印迹分析.

SCF 抗体 (G-3): sc-13126

4.3(13)
寫評論提問

说明书
  • SCF 抗体 G-3 是小鼠单克隆 IgG2b κ，SCF抗体, 在53篇文献中引用，规格为200 µg/ml
  • 免疫human物种的stem cell factor (SCF)的氨基酸26-214
  • SCF 抗体 (G-3) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 SCF
  • 抗SCF抗体（G-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取SCF (G-3): sc-13126的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 SCF 抗体 (G-3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与SCF 抗体 (G-3) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

SCF抗体（G-3）是一种小鼠单克隆IgG2b kappa轻链抗体，可通过WB、IP、IF、IHCP和ELISA检测小鼠、大鼠和人源SCF蛋白。SCF抗体（G-3）有非结合型和多种结合型，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®结合型。SCF作为跨膜酪氨酸激酶受体原癌基因c-Kit的配体，在多种细胞过程中发挥着至关重要的作用。SCF是一种多效细胞因子，在人类和小鼠体内分别由248个和220个氨基酸组成，以两种可替代的剪接形式存在。较大的SCF形式主要在成纤维细胞、大脑和胸腺中表达，而较小的变异形式则存在于脾脏、睾丸、胎盘和小脑等组织中。SCF对于生殖细胞、造血祖细胞和黑色素细胞前体的发育至关重要，因为SCF能够刺激成熟和未成熟肥大细胞的增殖和成熟。SCF与c-Kit的相互作用对于包括造血和色素沉着在内的各种生物过程至关重要，这使得SCF单克隆抗体（G-3）成为发育生物学和癌症研究领域的重要研究工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

SCF 抗体 (G-3) 参考文献:

  1. KL-1 和 KL-2 两种细胞缔合作用形式的差异表达和处理：KL-1 和 KL-2。  |  Huang, EJ., et al. 1992. Mol Biol Cell. 3: 349-62. PMID: 1378327
  2. 小鼠基质细胞选择性地表达膜结合型和分泌型的人类同源钢基因产物--干细胞因子，从而在长期骨髓培养中支持人类造血。  |  Toksoz, D., et al. 1992. Proc Natl Acad Sci U S A. 89: 7350-4. PMID: 1380155
  3. 膜锚定生长因子的裂解涉及通过共同机制调节的不同蛋白酶活性。  |  Pandiella, A., et al. 1992. J Biol Chem. 267: 24028-33. PMID: 1385433
  4. 肥大细胞生长因子位于小鼠第 10 号染色体的钢基因座附近，在一些钢等位基因中被删除。  |  Copeland, NG., et al. 1990. Cell. 63: 175-83. PMID: 1698554
  5. 大鼠和人类干细胞因子 DNA 的初级结构和功能表达。  |  Martin, FH., et al. 1990. Cell. 63: 203-11. PMID: 2208279
  6. 干细胞因子在胎盘微环境中的作用。  |  Khodadi, E., et al. 2016. Cell Tissue Res. 366: 523-531. PMID: 27234501
  7. 促红细胞生成素、干细胞因子和癌细胞迁移。  |  Vazquez-Mellado, MJ., et al. 2017. Vitam Horm. 105: 273-296. PMID: 28629522
  8. 骨髓脂肪细胞通过分泌 SCF 促进干细胞再生和造血。  |  Zhou, BO., et al. 2017. Nat Cell Biol. 19: 891-903. PMID: 28714970
  9. 干细胞因子：骨髓脂肪细胞和造血细胞之间的桥梁。  |  Li, Z. and MacDougald, OA. 2019. Haematologica. 104: 1689-1691. PMID: 31473604
  10. 抑制可溶性干细胞因子可促进肠粘膜修复。  |  Garcia-Hernandez, V., et al. 2023. Inflamm Bowel Dis. 29: 1133-1144. PMID: 36688460
  11. 干细胞因子和cKIT调节缺氧状态下的内皮细胞糖酵解。  |  Jeong, H., et al. 2024. Cardiovasc Res. 120: 745-755. PMID: 38507654
  12. 在有 SCF 的情况下培养单核细胞不会制造肥大细胞。循环肥大细胞祖细胞的特征为 c-kit+, CD34+, Ly-, CD14-, CD17-, 集落形成细胞。  |  Agis, H., et al. 1993. J Immunol. 151: 4221-7. PMID: 7691941

订购信息

产品名称产品编号规格价格数量收藏夹

SCF 抗体 (G-3)

sc-13126
200 µg/ml
$316.00

SCF (G-3): m-IgGκ BP-HRP 套装

sc-520598
200 µg Ab, 40 µg BP
$354.00

SCF (G-3): m-IgG2b BP-HRP 套装

sc-548841
200 µg Ab; 10 µg BP
$354.00

SCF 抗体 (G-3) AC

sc-13126 AC
500 µg/ml, 25% agarose
$416.00

SCF 抗体 (G-3) HRP

sc-13126 HRP
200 µg/ml
$316.00

SCF 抗体 (G-3) FITC

sc-13126 FITC
200 µg/ml
$330.00

SCF 抗体 (G-3) PE

sc-13126 PE
200 µg/ml
$343.00

SCF 抗体 (G-3) Alexa Fluor® 488

sc-13126 AF488
200 µg/ml
$357.00

SCF 抗体 (G-3) Alexa Fluor® 546

sc-13126 AF546
200 µg/ml
$357.00

SCF 抗体 (G-3) Alexa Fluor® 594

sc-13126 AF594
200 µg/ml
$357.00

SCF 抗体 (G-3) Alexa Fluor® 647

sc-13126 AF647
200 µg/ml
$357.00

SCF 抗体 (G-3) Alexa Fluor® 680

sc-13126 AF680
200 µg/ml
$357.00

SCF 抗体 (G-3) Alexa Fluor® 790

sc-13126 AF790
200 µg/ml
$357.00

Do you recommend using reduced or non-reduced cell lysates?

Asked by: raniagh
Thank you for your inquiry. This protein is subject to post-translational modifications, which can also cause disulfide bonds to form. As such, using a reducing sample buffer is advisable.
Answered by: Tech Support Europe
Date published: 2024-05-29

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 3
Date published: 2017-01-13

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with SCF (G-3): sc-13126?

Asked by: jerojero
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 3
Date published: 2017-01-13
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Rated 3 out of 5 by from 免疫組織化学染色はまずまず精巣のパラフィン切片を使って免疫蛍光染色を行ったところ、SCFを検出できた。 しかし、特異性に関して問題があると思われる。
Date published: 2020-12-23
Rated 4 out of 5 by from ICH stainingI applied for a sample and used it for ICH staining. The result is not stable.
Date published: 2017-06-28
Rated 5 out of 5 by from Perfect!I applied for a sample one month ago and used it 2 times. I was so ecxited that the band was very clear, so was the background.
Date published: 2017-05-28
Rated 5 out of 5 by from Great productOutstanding antibody for IHC staining; also strong WB
Date published: 2017-01-18
Rated 5 out of 5 by from Works Great!This antibody works great in western blot analysis of SCF expression in MCF7 and COLO 320DM whole cell lysates and rat brain, mouse brain, human colon and human lung tissue extracts.
Date published: 2016-12-23
Rated 5 out of 5 by from Excellent antibody for WBWe observed single 45 kDa band of SCF in WB. Dilution of primary antibody is 1: 500. Samples are whole cell lysates of rat bone marrow mesenchymal stem cells.
Date published: 2016-12-12
Rated 4 out of 5 by from Pleased with our IHC resultsPleased with our IHC results.
Date published: 2015-07-21
Rated 4 out of 5 by from Robust signal in WB in human recombinant SCFRobust signal in WB in human recombinant SCF. -SCBT QC
Date published: 2015-05-06
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SCF 抗体 (G-3) is rated 4.3 out of 5 by 13.
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