Sarcospan Antibody (E-2): sc-393187

Sarcospan Antibody (E-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects Sarcospan protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Sarcospan antibody (E-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Sarcospan, also known as K-Ras oncogene-associated protein, plays a crucial role in the dystrophin-glycoprotein complex (DGC), which is essential for maintaining muscle integrity and function. Sarcospan is primarily located in the sarcolemma of striated muscle cells, where Sarcospan forms a vital link between the subsarcolemmal cytoskeleton and the extracellular matrix. Proper localization of Sarcospan is critical, as defects in DGC components can lead to muscular dystrophy, highlighting Sarcospan′s importance in muscle viability. Sarcospan contains four transmembrane spanning helices with intracellular N- and C-terminal domains, and Sarcospan expression is significantly reduced in muscle tissues from patients with Duchenne muscular dystrophy. Sarcospan mRNAs are expressed across various cell lines, tumors, and normal tissues, with particularly high levels in muscle. Two isoforms, SPN1 and SPN2, arise from alternative splicing, with SPN1 predominantly found in heart and skeletal muscle, while SPN2 is expressed in a broader range of tissues, including the thymus, prostate, testis, ovary, small intestine, colon, and spleen. The sarcoglycan complex, which is integrally associated with Sarcospan in the DGC, is a heterotetrameric unit that also interacts with the signaling protein neural nitric oxide synthase through α-Syntrophin, further emphasizing Sarcospan′s multifaceted role in muscle function and signaling pathways.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Sarcospan Antibody (E-2) References:

1. Sarcospan-deficient mice maintain normal muscle function.  |  Lebakken, CS., et al. 2000. Mol Cell Biol. 20: 1669-77. PMID: 10669744
2. Maturation and maintenance of the neuromuscular synapse: genetic evidence for roles of the dystrophin--glycoprotein complex.  |  Grady, RM., et al. 2000. Neuron. 25: 279-93. PMID: 10719885
3. Biochemical evidence for association of dystrobrevin with the sarcoglycan-sarcospan complex as a basis for understanding sarcoglycanopathy.  |  Yoshida, M., et al. 2000. Hum Mol Genet. 9: 1033-40. PMID: 10767327
4. Expression of gamma -sarcoglycan in smooth muscle and its interaction with the smooth muscle sarcoglycan-sarcospan complex.  |  Barresi, R., et al. 2000. J Biol Chem. 275: 38554-60. PMID: 10993904
5. Coamplification in tumors of KRAS2, type 2 inositol 1,4,5 triphosphate receptor gene, and a novel human gene, KRAG.  |  Heighway, J., et al. 1996. Genomics. 35: 207-14. PMID: 8661122
6. Sarcospan, the 25-kDa transmembrane component of the dystrophin-glycoprotein complex.  |  Crosbie, RH., et al. 1997. J Biol Chem. 272: 31221-4. PMID: 9395445

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Sarcospan Antibody (E-2) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-Sarcospan Antibody (E-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Sarcospan Antibody (E-2) Specifications

Alternate Product Name: Sarcospan

Product Clone Name: E-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 162-199

Epitope Target: Sarcospan

Epitope Species: human

Product Reactivity/Detection: Sarcospan

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for Sarcospan Antibody (E-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin