Date published: 2025-9-6

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Rtn-3 抗体 (F-6): sc-374599

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说明书
  • Rtn-3 抗体 F-6 是小鼠单克隆 IgG1 κ,Rtn-3抗体, 在15篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列1-90位于human物种的Rtn-3的N-terminus
  • Rtn-3 抗体 (F-6) 推荐用于WB, IP, IF 和 ELISA,检测human 来源的 Rtn-3
  • 抗Rtn-3抗体(F-6)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Rtn-3 (F-6): sc-374599免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP是Rtn-3 Antibody (F-6) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与Rtn-3 Antibody (F-6) 搭配使用(请参阅下面的订购信息)。

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関連項目

Rtn-3抗体(F-6)是一种IgG1 κ小鼠单克隆Rtn-3抗体(也称为Rtn-3抗体),可通过WB、IP、IF和ELISA检测人源Rtn-3蛋白。Rtn-3抗体(F-6)既有非偶联型抗Rtn-3抗体形式,也有多种偶联形式的抗Rtn-3抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Reticulon(Rtn)家族由四个成员组成:Rtn-1(也称为神经内分泌特异性蛋白或NSP)、Rtn-2(也称为NSP样-1)、Rtn-3(也称为NSP样-2)和Nogo(也称为Rtn-4A)。Reticulon蛋白通过其共同的C末端区域锚定在内质网膜上。Rtn-1基因定位于人染色体14q23.1,表达为三种异构体:Rtn-1A(NSP-A)、Rtn-1B(NSP-B)和Rtn-1C(NSP-C)。编码人Rtn-2的基因位于染色体19q13上,也编码三种异构体。Rtn-2-A和Rtn-2-C是通过使用不同的起始位点产生的,而Rtn-2-B是Rtn-2-A异构体的选择性剪接变体。Rtn-2-A和Rtn-2-B在脑中高度表达,而Rtn-2-C主要在骨骼肌中表达。人Rtn-3基因位于染色体11q13上,广泛表达,在脑中的表达量最高。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Rtn-3 抗体 (F-6) 参考文献:

  1. 网状结构基因家族新成员(RTN3)的克隆:基因结构和在 11q13 的染色体定域。  |  Moreira, EF., et al. 1999. Genomics. 58: 73-81. PMID: 10331947
  2. 鉴定 Nogo 轴突再生抑制因子为网状结构蛋白  |  GrandPré, T., et al. 2000. Nature. 403: 439-44. PMID: 10667797
  3. 小细胞肺癌中神经内分泌特异性蛋白 B(NSP-B)的亚细胞定域和超分子结构。  |  Senden, NH., et al. 1994. Eur J Cell Biol. 65: 341-53. PMID: 7720728
  4. NSP编码的网状细胞是诊断人类肺癌的新型神经内分泌标志物。  |  van de Velde, HJ., et al. 1994. Cancer Res. 54: 4769-76. PMID: 8062278
  5. 一种主要表达肌肉和神经的新型小鼠基因的分子克隆。  |  Geisler, JG., et al. 1998. Mamm Genome. 9: 274-82. PMID: 9530622
  6. 神经元分化伴随着 NSP-C 的表达。  |  Hens, J., et al. 1998. Cell Tissue Res. 292: 229-37. PMID: 9560466
  7. 人类 RTN2 基因的 cDNA 克隆, 基因组组织和表达,RTN2 基因是编码网状结构子的基因家族成员之一。  |  Roebroek, AJ., et al. 1998. Genomics. 51: 98-106. PMID: 9693037

订购信息

产品名称产品编号规格价格数量收藏夹

Rtn-3 抗体 (F-6)

sc-374599
200 µg/ml
$316.00

Rtn-3 (F-6): m-IgG Fc BP-HRP 套装

sc-552030
200 µg Ab; 10 µg BP
$354.00

Rtn-3 抗体 (F-6) AC

sc-374599 AC
500 µg/ml, 25% agarose
$416.00

Rtn-3 抗体 (F-6) HRP

sc-374599 HRP
200 µg/ml
$316.00

Rtn-3 抗体 (F-6) FITC

sc-374599 FITC
200 µg/ml
$330.00

Rtn-3 抗体 (F-6) PE

sc-374599 PE
200 µg/ml
$343.00

Rtn-3 抗体 (F-6) Alexa Fluor® 488

sc-374599 AF488
200 µg/ml
$357.00

Rtn-3 抗体 (F-6) Alexa Fluor® 546

sc-374599 AF546
200 µg/ml
$357.00

Rtn-3 抗体 (F-6) Alexa Fluor® 594

sc-374599 AF594
200 µg/ml
$357.00

Rtn-3 抗体 (F-6) Alexa Fluor® 647

sc-374599 AF647
200 µg/ml
$357.00

Rtn-3 抗体 (F-6) Alexa Fluor® 680

sc-374599 AF680
200 µg/ml
$357.00

Rtn-3 抗体 (F-6) Alexa Fluor® 790

sc-374599 AF790
200 µg/ml
$357.00

What is the recommended fixation method for immunofluorescence staining with Rtn-3 (F-6): sc-374599 monoclonal antibody?

Asked by: Professor Griffin
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from very good antibody for human cellsVery good results for use in Western Blot or immunofluorescence
Date published: 2020-02-11
Rated 4 out of 5 by from Beautiful staining of RTN-3This antibodies give a beautiful staining of RTN-3 with Methanol fixation. I also tested with PFA fixation and triton x-100 permeabilization but not successful, so I recommend Methanol fixation (5 minutes at -20).
Date published: 2017-09-30
Rated 5 out of 5 by from Worked well for western blotI used Hela cell and U2OS cell lysates and probed for this antibody at 1:2000. Works really well for western blot.
Date published: 2017-08-29
Rated 5 out of 5 by from excellent antibody forWBI try this sample product, WB with 1:1000 dilution, I got very clear bands!
Date published: 2017-08-15
Rated 5 out of 5 by from Solid antibody for Western blotThis is a good choice if you need a reliable Rtn-3 antibody for Western blot.
Date published: 2017-02-02
Rated 5 out of 5 by from Produced great immunofluorescence cytoplasmicProduced great immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-02-05
Rated 5 out of 5 by from Produced positive Western Blot data of RtnProduced positive Western Blot data of Rtn-3 expression in IMR-32 whole cell lysate. -SCBT QC
Date published: 2013-04-12
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Rtn-3抗体 (F-6) is rated 4.9 out of 5 by 7.
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