Date published: 2025-9-7

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rRNA 抗体 (Y10b): sc-33678

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说明书
  • rRNA 抗体 Y10b 是小鼠单克隆 IgG2a κ,rRNA抗体, 在15篇文献中引用,规格为200 µg/ml
  • 源自未免疫的自身免疫性NZB/NZW小鼠
  • rRNA 抗体 (Y10b) 推荐用于 IP, IF 和 IHC(P),检测mouse, rat 和human 来源的 rRNA
  • 抗rRNA抗体(Y10b)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取rRNA (Y10b): sc-33678免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP是rRNA Antibody (Y10b) 适用于 IHC(P) 应用。 的首选辅助检测试剂。该试剂现与rRNA Antibody (Y10b) 搭配使用(请参阅下面的订购信息)。

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関連項目

rRNA抗体(Y10b)是一种小鼠单克隆IgG2a kappa轻链抗体,可通过免疫沉淀(IP)和免疫荧光(IF)检测小鼠、大鼠和人类来源的rRNA蛋白。抗rRNA抗体(Y10 b)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。核糖体RNA(rRNA)是核糖体的重要组成部分,在氨基酸合成多肽的过程中起着至关重要的作用,最终导致蛋白质的合成。核糖核酸的结构尤为重要,因为核糖核酸是核糖体结构的核心,为核糖体蛋白的组装提供了支架。真核生物的核糖体由四个不同的核糖核酸分子组成:5S、5.8S、18S和28S,每个分子都在不同的细胞区室中合成,其中5.8S、18S和28S在核仁中合成,5S在细胞核中合成。这些rRNA分子不仅有助于核糖体的结构完整性,而且有助于关键功能的发挥,例如正确定位信使RNA和肽转移酶活性,这对于翻译过程中的肽键形成至关重要。rRNA的丰度约占典型真核细胞中总RNA的80%,这凸显了rRNA在细胞生物学中的基础作用以及rRNA抗体(Y10b)在研究核糖体功能和蛋白质合成中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

rRNA 抗体 (Y10b) 参考文献:

  1. 发育中的大鼠嗅球中核糖体 RNA 的表达  |  Fiske, BK. and Brunjes, PC. 1999. Brain Res Dev Brain Res. 113: 55-60. PMID: 10064874
  2. 海马神经元局部蛋白质合成的动态可视化。  |  Aakalu, G., et al. 2001. Neuron. 30: 489-502. PMID: 11395009
  3. 乌贼巨轴突中的核糖体  |  Bleher, R. and Martin, R. 2001. Neuroscience. 107: 527-34. PMID: 11719007
  4. Ⅰ和Ⅱ组代谢型谷氨酸受体对小鸡听觉神经元核糖体的活动依赖性调节是必要的。  |  Nicholas, AH. and Hyson, RL. 2004. Brain Res. 1014: 110-9. PMID: 15212997
  5. 利用核糖体标记测量树突状 mRNA 运输。  |  Kim, HK., et al. 2005. Biochem Biophys Res Commun. 328: 895-900. PMID: 15707962
  6. 核糖体 RNA 表位的蛋白质掩蔽是传入剥夺诱导神经元死亡的早期事件。  |  Garden, GA., et al. 1995. Mol Cell Neurosci. 6: 293-310. PMID: 7496633

订购信息

产品名称产品编号规格价格数量收藏夹

rRNA 抗体 (Y10b)

sc-33678
200 µg/ml
$316.00

rRNA (Y10b): m-IgG Fc BP-HRP 套装

sc-525542
200 µg Ab; 10 µg BP
$354.00

rRNA 抗体 (Y10b) AC

sc-33678 AC
500 µg/ml, 25% agarose
$416.00

rRNA 抗体 (Y10b) HRP

sc-33678 HRP
200 µg/ml
$316.00

rRNA 抗体 (Y10b) FITC

sc-33678 FITC
200 µg/ml
$330.00

rRNA 抗体 (Y10b) PE

sc-33678 PE
200 µg/ml
$343.00

rRNA 抗体 (Y10b) Alexa Fluor® 488

sc-33678 AF488
200 µg/ml
$357.00

rRNA 抗体 (Y10b) Alexa Fluor® 546

sc-33678 AF546
200 µg/ml
$357.00

rRNA 抗体 (Y10b) Alexa Fluor® 594

sc-33678 AF594
200 µg/ml
$357.00

rRNA 抗体 (Y10b) Alexa Fluor® 647

sc-33678 AF647
200 µg/ml
$357.00

rRNA 抗体 (Y10b) Alexa Fluor® 680

sc-33678 AF680
200 µg/ml
$357.00

rRNA 抗体 (Y10b) Alexa Fluor® 790

sc-33678 AF790
200 µg/ml
$357.00

What is the exact epitope used to make this antibody?

Asked by: Maria2020
Thank you for your inquiry. This antibody was derived from unimmunized autoimmune NZB/NZW mice. Unfortunately the exact epitope has not been mapped for this antibody, so no further information is available.
Answered by: Tech Service
Date published: 2020-02-04

What is the recommended fixation method for immunofluorescence staining with rRNA (Y10b): sc-33678 monoclonal antibody?

Asked by: Germaine
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Specific and clear antibodyI used this antibody for immunofluorescence staining to track the ribosomes in the sectioned slices of the intestinal samples, the picture is clear and seems reasonable, the results correspond with our western blot results, but I didn't check the high resolution, so here I would like to say it's good to use for the mouse tissue sample staining purpose.
Date published: 2017-09-10
Rated 5 out of 5 by from Good antibodyThis is a strong antibody for rRNA studies, it's specific and reliable. Highly recommend!
Date published: 2017-02-06
Rated 4 out of 5 by from This antibody was used in a publication forThis antibody was used in a publication for IHCP on human renal tissue microarrays (TMAs) - SCBT Publication Review
Date published: 2015-05-17
Rated 4 out of 5 by from Clear immunofluorescence cytoplasmic andClear immunofluorescence cytoplasmic and nuclear staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-04-16
Rated 4 out of 5 by from IF publishable data with mouse primary embryonicIF publishable data with mouse primary embryonic motoneurons - SCBT Publication Review
Date published: 2014-01-04
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rRNA 抗体 (Y10b) is rated 4.2 out of 5 by 5.
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