Date published: 2025-9-6

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RPA 14 kDa subunit 抗体 (E-2): sc-271564

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说明书
  • RPA 14 kDa subunit抗体(E-2)是小鼠单克隆IgG1 (kappa轻链),规格为200 µg/ml
  • 针对human起源的RPA 14 kDa subunit内部区域内的氨基酸24-81进行
  • 推荐用于 mouse, rat 和 human 来源的RPA 14 kDa subunit WB, IP, IF 和 ELISA检测
  • 关于如何获取RPA 14 kDa subunit (E-2): sc-271564免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是RPA 14 kDa subunit Antibody (E-2) for WB applications. 的首选辅助检测试剂,这些试剂现与RPA 14 kDa subunit Antibody (E-2) 打包提供(请参阅下面的订购信息)。

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    RPA 14 kDa亚基抗体(E-2)是一种IgG1 κ小鼠单克隆RPA 14 kDa亚基抗体,可通过WB、IP、IF和ELISA检测小鼠、大鼠和人源的RPA 14 kDa亚基。RPA 14 kDa亚基抗体(E-2)为非偶联型抗RPA 14 kDa亚基抗体。单链DNA结合蛋白(SSB)对于原核细胞、真核细胞、线粒体、噬菌体和病毒中的DNA功能至关重要。复制蛋白A(RPA)是一种高度保守的真核蛋白,是一种由三个亚基组成的异源三聚体SSB,分别称为RPA 14 kDa(也称为RPA3)、RPA 32 kDa和RPA 70 kDa。这些亚基共同在DNA复制、重组和修复中发挥重要作用。RPA是核苷酸切除修复早期阶段涉及的主要损伤识别结构之一,并可能在端粒维持中发挥作用。人RPA(hRPA)与DNA的结合涉及分子极性,其中初始hRPA结合发生在ssDNA底物的5′侧,然后向3′方向延伸,形成稳定结合的hRPA。RPA 14 kDa亚基定位于细胞核,是RPA复合物中最小的组分,与其他亚基一起调节DNA代谢的各个方面。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    RPA 14 kDa subunit 抗体 (E-2) 参考文献:

    1. 通过 ATRIP 识别 RPA-ssDNA 复合物来感知 DNA 损伤。  |  Zou, L. and Elledge, SJ. 2003. Science. 300: 1542-8. PMID: 12791985
    2. 复制蛋白 A 缺陷细胞中的 DNA 复制缺陷, 自发性 DNA 损伤和 ATM 依赖性检查点激活。  |  Dodson, GE., et al. 2004. J Biol Chem. 279: 34010-4. PMID: 15197179
    3. 复制蛋白 A 与 AID 相互作用,促进体细胞高突变靶标的脱氨。  |  Chaudhuri, J., et al. 2004. Nature. 430: 992-8. PMID: 15273694
    4. RPA 样蛋白介导酵母端粒功能  |  Gao, H., et al. 2007. Nat Struct Mol Biol. 14: 208-14. PMID: 17293872
    5. 发现 62 个 DNA 修复基因的多态性以及单倍型与肺癌和头颈癌风险的缔合作用。  |  Michiels, S., et al. 2007. Carcinogenesis. 28: 1731-9. PMID: 17494052
    6. 人类 RPA1 的细胞功能。结构域在复制, 修复和检查点中的多重作用。  |  Haring, SJ., et al. 2008. J Biol Chem. 283: 19095-111. PMID: 18469000
    7. 人类三个复制蛋白 A 基因(RPA1, RPA2 和 RPA3)的高分辨率基因组拆分图谱。  |  Umbricht, CB., et al. 1994. Genomics. 20: 249-57. PMID: 8020972
    8. 人类复制蛋白 A 的 14 kDa 亚基的克隆, 过表达和基因组图谱。  |  Umbricht, CB., et al. 1993. J Biol Chem. 268: 6131-8. PMID: 8454588

    订购信息

    产品名称产品编号规格价格数量收藏夹

    RPA 14 kDa subunit 抗体 (E-2)

    sc-271564
    200 µg/ml
    $316.00

    RPA 14 kDa subunit (E-2): m-IgG Fc BP-HRP 套装

    sc-527258
    200 µg Ab; 10 µg BP
    $354.00

    RPA 14 kDa subunit (E-2): m-IgG1 BP-HRP 套装

    sc-532631
    200 µg Ab; 20 µg BP
    $354.00

    I need to use an antibody to detect the protein in samples of monkey origin. Do you know if sc-271564: RPA 14 kDa subunit (E-2) mouse monoclonal antibody will work?

    Asked by: DefinitelyNotMatt
    Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
    Answered by: Technical Support
    Date published: 2017-03-06
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    Rated 5 out of 5 by from greatI used this antiboty 1:500 for WB,the band is clear and have no background
    Date published: 2017-09-23
    Rated 5 out of 5 by from Great for WBWorks great for WB at 1:1000 dilution in HeLa and 293T cells.
    Date published: 2017-09-06
    Rated 4 out of 5 by from Used this in IF and got good stainingUsed this in IF and got good staining.
    Date published: 2015-09-11
    Rated 5 out of 5 by from Great Western Blot data of RPA 14 kDa subunitGreat Western Blot data of RPA 14 kDa subunit expression in HL-60 and K-562 whole cell lysates and HL-60 and MOLT-4 nuclear extracts. -SCBT QC
    Date published: 2015-04-25
    Rated 5 out of 5 by from Good Western Blot data of RPA 14 kDa subunitGood Western Blot data of RPA 14 kDa subunit expression in K-562 and HL-60 nuclear extracts and K-562 whole cell lysate . -SCBT QC
    Date published: 2013-05-27
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    RPA 14 kDa subunit 抗体 (E-2) is rated 4.8 out of 5 by 5.
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