RIPA Lysis Buffer System A high quality, ready to use mammalian cell and tissue lysis buffer with protease inhibitors included.

RIPA Lysis Buffer System

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应用; RIPA Lysis Buffer System® is a high quality, ready to use mammalian cell and tissue lysis buffer with protease inhibitors included
* 参考分析证明 大量特定数据 (包括水 含量).
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RIPA (Radioimmunoprecipitation) Lysis Buffer System is used to lyse cells and tissue, for radio immunoprecipitation assay (RIPA). RIPA lysis buffer has stronger denaturing capabilities than NP-40 (sc-281108) or Triton X-100 (sc-29112) and is particularly useful for disruption of nuclear membranes in the preparation of nuclear extracts.

This high quality product includes protease inhibitors, making it ready for use in mammalian cell and tissue lysis.

sc-24948, 50 mL - Components supplied in four vials:
VIAL 1: 50 mL 1X lysis buffer (pH 7.4 ±0.1)
VIAL 2: 500 μL (200mM) PMSF in DMSO
VIAL 3: 500 μL protease inhibitor cocktail in DMSO
VIAL 4: 500 μL (100mM) sodium orthovanadate in water

sc-24948A, 500 mL - Components supplied in four vials:
VIAL 1: 500 mL 1X lysis buffer
VIAL 2: 5 ml (200mM) PMSF in DMSO
VIAL 3: 5 ml protease inhibitor cocktail in DMSO
VIAL 4: 5 ml (100mM) sodium orthovanadate in water


参考文献

1. Poirier, F., et al. 1982. Int. J. Cancer. 29: 69-76. PMID: 6277805

2. DeSeau, V., et al. 1987. J. Cell. Biochem. 35: 113-128. PMID: 2448318

3. Bolen, J.B., et al. 1987. Oncogene Res. 1: 149-168. PMID: 2453014

4. Ngoka, L.C. 2008. Proteome Sci. 6: 30. PMID: 18950484

用法 :
1. Immediately prior to lysing cells, combine 10 μl PMSF solution, 10 μl sodium orthovanadate solution and 10-20 μl protease inhibitor cocktail solution per ml of 1X RIPA Lysis buffer to prepare complete RIPA.

2. Use 3 ml complete RIPA per gram of tissue

- OR 1 ml complete RIPA per 2.0x107 cells in suspension

- OR 0.6 ml complete RIPA per subconfluent monolayer on a 100 mm plate.
物理状态 :
Liquid
保存 :
Store at -20° C
仅供科研使用。不可用于诊断或治疗。

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PMID: # 31279091  Huang, J.|Liu, W.|Doycheva, DM.|Gamdzyk, M.|Lu, W.|Tang, J.|Zhang, JH.| et al. 2019. Free Radic. Biol. Med. 141: 322-337.

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PMID: # 31545452  2019. Oncol. Rep.

PMID: # 31325432  Agrawal, L.|Vimal, SK.|Shiga, T.| et al. 2019. Neuropharmacology. 158: 107712.

PMID: # 31278615  2019. Cardiovasc. Toxicol.

PMID: # 31241772  Mansoori, B.|Mohammadi, A.|Naghizadeh, S.|Gjerstorff, M.|Shanehbandi, D.|Shirjang, S.|Najafi, S.|Holmskov, U.|Khaze, V.|Duijf, PHG.|Baradaran, B.| et al. 2019. J. Cell. Physiol.

PMID: # 31114375  Rong, X.|Han, Q.|Lin, X.|Kremerskothen, J.|Wang, E.| et al. 2019. Cancer Manag Res. 11: 3395-3410.

PMID: # 31273064  Jain, A.|Agostini, LC.|McCarthy, GA.|Chand, SN.|Ramirez, A.|Nevler, A.|Cozzitorto, J.|Schultz, CW.|Lowder, CY.|Smith, KM.|Waddell, ID.|Raitses-Gurevich, M.|Stossel, C.|Gorman, YG.|Atias, D.|Yeo, CJ.|Winter, JM.|Olive, KP.|Golan, T.|Pishvaian, MJ.|Ogilvie, D.|James, DI.|Jordan, AM.|Brody, JR.| et al. 2019. Cancer Res.

PMID: # 31099942  Xu, C.|Kasimumali, A.|Guo, X.|Lu, R.|Xie, K.|Zhu, M.|Qian, Y.|Chen, X.|Pang, H.|Wang, Q.|Fan, Z.|Dai, H.|Mou, S.|Ni, Z.|Gu, L.| et al. 2019. Nephrology (Carlton).

PMID: # 30698330  Sommerville, LJ.|Gorman, KL.|Snyder, SA.|Monroe, DM.|Hoffman, M.| et al. 2019. J. Thromb. Haemost. 17: 670-680.

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