Date published: 2025-9-7

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RICK 抗体 (25): sc-136059

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说明书
  • RICK 抗体 25 是小鼠单克隆 IgG1, 在2篇文献中引用,提供 200 µg/ml
  • 免疫human物种的RICK的氨基酸333-532
  • 推荐用于 mouse, rat, human 和 canine 来源的RICK WB, IP 和 IF检测
  • 看 RICK (A-10): sc-166765,可查看有标记的 RICK 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 关于如何获取RICK (25): sc-136059免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 1 BP-HRP">m-IgG1 BP-HRP是RICK Antibody (25) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与RICK Antibody (25) 打包提供(请参阅下面的订购信息)。

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RICK 抗体 (25) 是一种小鼠单克隆 IgG1 抗体,可通过免疫印迹 (WB)、免疫沉淀 (IP) 和免疫荧光 (IF) 等应用检测小鼠、大鼠、人和犬样本中的 RICK。RICK 抗体 (25) 以非结合形式提供,可用于多种实验。RICK 又称 RIP2 或 CARDIAK,在细胞凋亡调控和核因子卡巴 B(NFkB)激活过程中起着至关重要的作用,而这是细胞应对应激和炎症的重要过程。RICK 主要位于细胞质中,在细胞质中 RICK 与各种信号复合体相互作用,特别是与肿瘤坏死因子受体(TNFR)相关的信号复合体。这种定位至关重要,因为 RICK 能有效介导信号通路,根据具体情况导致细胞存活或死亡。这些复合物中的 RICK 有助于招募其他蛋白,如 TNFR 相关因子(TRAF)家族中的蛋白,这些蛋白对于决定细胞结果的下游信号事件至关重要。了解 RICK 的功能和定位对于阐明 RICK 在以凋亡和炎症失调为特征的疾病中的作用非常重要,这使得抗 RICK 抗体 (25) 成为研究人员研究这些通路的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

RICK 抗体 (25) 参考文献:

  1. RICK/Rip2/CARDIAK 介导先天性和适应性免疫系统受体的信号传导。  |  Kobayashi, K., et al. 2002. Nature. 416: 194-9. PMID: 11894098
  2. RICK/RIP2 通过 Nod1 和 Nod2 而非 TLRs 介导先天性免疫反应。  |  Park, JH., et al. 2007. J Immunol. 178: 2380-6. PMID: 17277144
  3. NOD2-RICK 复合物从质膜发出信号。  |  Lécine, P., et al. 2007. J Biol Chem. 282: 15197-207. PMID: 17355968
  4. 受体相互作用蛋白激酶-3决定细胞对TNF-α的坏死反应。  |  He, S., et al. 2009. Cell. 137: 1100-11. PMID: 19524512
  5. 抑制受体相互作用蛋白激酶 1 可改善实验性非酒精性脂肪肝。  |  Majdi, A., et al. 2020. J Hepatol. 72: 627-635. PMID: 31760070
  6. 受体相互作用蛋白激酶 2 是胰腺癌的免疫疗法靶点  |  Sang, W., et al. 2024. Cancer Discov. 14: 326-347. PMID: 37824278
  7. 由 TNF 相关细胞因子和受体家族介导的细胞凋亡。  |  Ware, CF., et al. 1996. J Cell Biochem. 60: 47-55. PMID: 8825415
  8. Apo-3 是肿瘤坏死因子受体家族的新成员,它含有一个死亡结构域,能激活细胞凋亡和 NF-kappa B。  |  Marsters, SA., et al. 1996. Curr Biol. 6: 1669-76. PMID: 8994832
  9. TRAF2 对 JNK 而非 NF-kappaB 的激活至关重要,并能调节淋巴细胞的增殖和存活。  |  Lee, SY., et al. 1997. Immunity. 7: 703-13. PMID: 9390693
  10. RICK是一种含有caspase招募结构域的新型蛋白激酶,它与CLARP相互作用并调节CD95介导的细胞凋亡。  |  Inohara, N., et al. 1998. J Biol Chem. 273: 12296-300. PMID: 9575181
  11. RIP2 是一种新型 NF-kappaB 激活和细胞死亡诱导激酶。  |  McCarthy, JV., et al. 1998. J Biol Chem. 273: 16968-75. PMID: 9642260
  12. 鉴定 CARDIAK,一种与 caspase-1 相关的 RIP 样激酶。  |  Thome, M., et al. 1998. Curr Biol. 8: 885-8. PMID: 9705938

订购信息

产品名称产品编号规格价格数量收藏夹

RICK 抗体 (25)

sc-136059
200 µg/ml
$316.00

RICK (25): m-IgG1 BP-HRP 套装

sc-531660
200 µg Ab; 20 µg BP
$354.00

Does this antibody work for IHC on frozen mouse tissue? can we buy this antibody conjugated to Alexa Fluor 488?

Asked by: Anonymous
Thank you for your question. RICK Antibody (25): sc-136059 is only recommended for detection of RICK of mouse, rat, human and canine origin by WB, IP and IF using cell samples rather than tissue sections. The antibody is currently not available as an AF488 conjugate.
Answered by: Technical Support Europe
Date published: 2020-10-16

I am using RICK (25): sc-136059 for immunofluorescence, what dilution of this antibody should I use?

Asked by: Trav11
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
  • y_2025, m_8, d_27, h_4CST
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Rated 5 out of 5 by from Produced superb immunofluorescence nuclearProduced superb immunofluorescence nuclear and cytoplasmic staining in HeLa cells. -SCBT QC
Date published: 2015-02-20
Rated 5 out of 5 by from Robust band observed in western blotting using SKRobust band observed in western blotting using SK-MEL-28 whole cell lysate. -SCBT QC
Date published: 2015-02-01
Rated 5 out of 5 by from StrongStrong, clean band in Western blot analysis of RICK expression in human endothelial whole cell lysate. -SCBT QC
Date published: 2015-01-22
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RICK 抗体 (25) is rated 5.0 out of 5 by 3.
  • y_2025, m_8, d_27, h_4
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