RhoC Double Nickase Plasmid (h): sc-401242-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RhoC Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- RhoC Double Nickase Plasmid (h) and RhoC Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting RHOC. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: RhoC Antibody (C-10): sc-393090
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RhoC Double Nickase Plasmid (h) | sc-401242-NIC | 20 µg | $410.00 | |||
RhoC Double Nickase Plasmid (h2) | sc-401242-NIC-2 | 20 µg | $410.00 |
RHOC encodes the small GTPase RhoC, a member of the Rho family that functions as a molecular switch cycling between GDP- and GTP-bound states to regulate actin cytoskeleton remodeling. RhoC signaling coordinates stress fiber assembly, focal adhesion dynamics, and cell contractility through effectors such as ROCK and mDia, integrating with pathways that control cell polarity and motility. Altered RHOC activity has been associated with invasive phenotypes in multiple tumor contexts and with changes in cytoskeletal organization that influence metastatic dissemination. RHOC is therefore widely used as a node for studying Rho GTPase networks, mechanotransduction, and migration-related signaling programs in human cells.
RhoC Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the RHOC locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within RHOC. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt RHOC function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of RHOC-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.