Date published: 2025-9-6

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RhoC 抗体 (C-10): sc-393090

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说明书
  • RhoC 抗体 C-10 是小鼠单克隆 IgG1 κ,RhoC抗体, 在4篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的Rho C的C-terminus的氨基酸158-189之间
  • RhoC 抗体 (C-10) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Rho C; 也和以下物种反应,包括: 和 equine, canine, bovine and porcine
  • 抗RhoC抗体(C-10)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取RhoC (C-10): sc-393090免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 RhoC 抗体 (C-10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与RhoC 抗体 (C-10) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Rho C抗体(C-10)是一种IgG1 κ小鼠单克隆Rho C抗体(也称为RHOC抗体或RHOH9抗体),可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的Rho C蛋白。Rho C抗体(C-10)既有非偶联形式的抗Rho C抗体,也有多种偶联形式的抗Rho C抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Ras p21鸟嘌呤核苷酸蛋白家族在信号转导通路中作用显著,在人类恶性肿瘤中突变频率高,使得该家族被广泛研究。然而,现在很清楚的是,Ras蛋白(H-、K-和N-Ras p21)是更大的相关蛋白超家族成员。该家族中六个成员,Rap 1A、Rap 1B、Rap 2、R-Ras、Ral A和Ral B,与Ras相比具有约50%的氨基酸同源性。六种哺乳动物Rho蛋白(Rho A、B、C、G、7和8)与Ras约有30%的同源性,并在多种细胞类型中表达。Ras p21和Rho p21以及Ras超家族其他成员都包含一个羧基末端CAAX序列(C,半胱氨酸;A,脂肪族氨基酸;X,任何氨基酸),在Ras中该序列对于正确定位和功能至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

RhoC 抗体 (C-10) 参考文献:

  1. p21rho 蛋白的翻译后修饰。  |  Adamson, P., et al. 1992. J Biol Chem. 267: 20033-8. PMID: 1400319
  2. 表皮生长因子通过激活Rho A和Rho C刺激人滋养层细胞迁移。  |  Han, J., et al. 2010. Endocrinology. 151: 1732-42. PMID: 20150581
  3. 成年小鼠组织中与 ras 相关的 ralA, rho12 和 rab 基因的表达。  |  Olofsson, B., et al. 1988. Oncogene. 3: 231-4. PMID: 2457862
  4. 鉴定 ras/R-ras 和 rho 调控蛋白的不同细胞质靶标。  |  Garrett, MD., et al. 1989. J Biol Chem. 264: 10-3. PMID: 2491843
  5. 在没有磷酸肌醇水解的情况下,用 ras 蛋白对瑞士 3T3 细胞进行刮擦加载可迅速激活蛋白激酶 C。  |  Morris, JD., et al. 1989. Oncogene. 4: 27-31. PMID: 2536916
  6. ras 超家族蛋白。  |  Chardin, P. 1988. Biochimie. 70: 865-8. PMID: 3145021
  7. 白血病相关Rho鸟嘌呤核苷酸交换因子和Ras同源家族成员C在胶质母细胞瘤细胞侵袭和抵抗中发挥作用  |  Ding, Z., et al. 2020. Am J Pathol. 190: 2165-2176. PMID: 32693062
  8. ras 基因。  |  Barbacid, M. 1987. Annu Rev Biochem. 56: 779-827. PMID: 3304147
  9. MicroRNA-20b-5p通过下调阿尔茨海默病Ras同源家族成员C加剧β-淀粉样蛋白诱导的神经细胞凋亡  |  Tian, Z., et al. 2021. Neurosci Lett. 742: 135542. PMID: 33278507
  10. 人类 rho cDNA 克隆 12 的核苷酸序列。  |  Yeramian, P., et al. 1987. Nucleic Acids Res. 15: 1869. PMID: 3822842
  11. 一个新的ras相关基因家族  |  Madaule, P. and Axel, R. 1985. Cell. 41: 31-40. PMID: 3888408
  12. 沉默Ras同源家族成员C对唾液腺腺样囊性癌的增殖、侵袭和迁移的影响。  |  Yu, W., et al. 2024. Hua Xi Kou Qiang Yi Xue Za Zhi. 42: 723-734. PMID: 39610069

订购信息

产品名称产品编号规格价格数量收藏夹

RhoC 抗体 (C-10)

sc-393090
200 µg/ml
$316.00

RhoC (C-10): m-IgG Fc BP-HRP 套装

sc-530351
200 µg Ab; 10 µg BP
$354.00

RhoC (C-10): m-IgGκ BP-HRP 套装

sc-523801
200 µg Ab, 40 µg BP
$354.00

RhoC (C-10): m-IgG1 BP-HRP 套装

sc-544006
200 µg Ab; 20 µg BP
$354.00

RhoC 抗体 (C-10) AC

sc-393090 AC
500 µg/ml, 25% agarose
$416.00

RhoC 抗体 (C-10) HRP

sc-393090 HRP
200 µg/ml
$316.00

RhoC 抗体 (C-10) FITC

sc-393090 FITC
200 µg/ml
$330.00

RhoC 抗体 (C-10) PE

sc-393090 PE
200 µg/ml
$343.00

RhoC 抗体 (C-10) Alexa Fluor® 488

sc-393090 AF488
200 µg/ml
$357.00

RhoC 抗体 (C-10) Alexa Fluor® 546

sc-393090 AF546
200 µg/ml
$357.00

RhoC 抗体 (C-10) Alexa Fluor® 594

sc-393090 AF594
200 µg/ml
$357.00

RhoC 抗体 (C-10) Alexa Fluor® 647

sc-393090 AF647
200 µg/ml
$357.00

RhoC 抗体 (C-10) Alexa Fluor® 680

sc-393090 AF680
200 µg/ml
$357.00

RhoC 抗体 (C-10) Alexa Fluor® 790

sc-393090 AF790
200 µg/ml
$357.00

RhoC (C-10) 中和勝肽

sc-393090 P
100 µg/0.5 ml
$68.00

What application is the blocking peptide sc-393090 P appropriate for?

Asked by: Professor Griffin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Excellent antibodyI bought this antibody a month ago. It works very well for western blot analysis.
Date published: 2017-12-06
Rated 5 out of 5 by from Anti Rho CExcelent antibody for western blot. Detected Rho C at expected size in 15 ug total proteine lysate, wet transfer one hour at 450 mAmps on PVDF membrane, overnight blocking with 5% nonfat dry milk in PBS, one hour incubation at RT with 1:500 anti Rho C in 5% PBS with 0,2%Tween followed by one hour incubation with 1:10.000 anti mouse HRP conjugated antibody and detection with ECL prime substrate followed by short film exposure (under 1 minute) and automatic processing.
Date published: 2017-09-25
Rated 4 out of 5 by from sharp bandsThis antibody was tested on human aortic endothelial cells post various treatments. It gives specific band with little background.
Date published: 2017-09-14
Rated 1 out of 5 by from Do not workI applyed for it two months ago and regretly it didn't work.
Date published: 2017-09-14
Rated 5 out of 5 by from Great choice for WB and IHCPExcellent results by WB and IHCP with Rho C Antibody (C-10)
Date published: 2017-01-25
Rated 5 out of 5 by from Worked well in WB in mouse 293T whole cell lysatesWorked well in WB in mouse 293T whole cell lysates. -SCBT QC
Date published: 2014-08-01
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RhoC 抗体 (C-10) is rated 4.0 out of 5 by 6.
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