Date published: 2025-9-6

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RhoB 抗体 (C-5): sc-8048

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说明书
  • RhoB 抗体 C-5 是小鼠单克隆 IgG1 κ,RhoB抗体, 在65篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的Rho B的内部区域内的氨基酸123-137之间
  • RhoB 抗体 (C-5) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Rho B p21; 也和以下物种反应,包括: 和 equine, canine, bovine and porcine
  • 抗RhoB抗体(C-5)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取RhoB (C-5): sc-8048免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG1 BP-HRP 是 RhoB 抗体 (C-5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与RhoB 抗体 (C-5) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Rho B 抗体(C-5)是一种小鼠单克隆 IgG1 kappa 轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、免疫组织化学和酶联免疫吸附测定(ELISA)检测小鼠、大鼠和人类的 Rho B。抗Rho B抗体(C-5)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。Rho B在调节肌动蛋白细胞骨架方面起着至关重要的作用,而肌动蛋白细胞骨架对于细胞迁移、粘附和分裂等各种细胞过程至关重要。Rho B的正常功能对于维持细胞完整性和促进细胞间交流至关重要,特别是在癌症进展的情况下,Rho B活性的改变会导致侵袭性和转移性增加。已知Rho B与几种关键蛋白相互作用,包括Rho相关蛋白激酶(ROCK)和各种鸟苷酸交换因子(GEFs),这进一步强调了Rho B在信号转导通路中的重要性。Rho B 中的羧基末端 CAAX 序列对于翻译后修饰和膜定位至关重要,可确保在细胞内发挥作用的正确定位。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

RhoB 抗体 (C-5) 参考文献:

  1. DNA 损伤后,新生物转化细胞的凋亡需要 RhoB 来介导。  |  Liu Ax,., et al. 2001. Proc Natl Acad Sci U S A. 98: 6192-7. PMID: 11353846
  2. RhoB 在小鼠发育过程中是不可或缺的,但它会改变肿瘤形成的易感性以及转化细胞的细胞粘附和生长因子信号转导。  |  Liu, AX., et al. 2001. Mol Cell Biol. 21: 6906-12. PMID: 11564874
  3. RhoB 通过防止有丝分裂后细胞死亡来控制 24 kDa FGF-2 诱导的 HeLa 细胞放射抗性。  |  Ader, I., et al. 2002. Oncogene. 21: 5998-6006. PMID: 12203112
  4. 雄激素对人类纤维肉瘤细胞增殖和细胞骨架重组的控制:RhoB 信号的作用。  |  Chauhan, S., et al. 2004. J Biol Chem. 279: 937-44. PMID: 14576147
  5. 表皮生长因子受体(EGFR), ErbB2 和 Ras(而非 Src)抑制 RhoB 的表达,而异位表达的 RhoB 可拮抗癌基因介导的转化。  |  Jiang, K., et al. 2004. Oncogene. 23: 1136-45. PMID: 14647415
  6. Akt 介导 Ras 下调 RhoB,RhoB 是转化, 入侵和转移的抑制因子。  |  Jiang, K., et al. 2004. Mol Cell Biol. 24: 5565-76. PMID: 15169915
  7. 为什么是三种 Rho 蛋白?RhoA, RhoB, RhoC 和细胞运动。  |  Wheeler, AP. and Ridley, AJ. 2004. Exp Cell Res. 301: 43-9. PMID: 15501444
  8. RhoB 和肌动蛋白聚合使 Src 激活与内膜介导的膜输送相协调。  |  Sandilands, E., et al. 2004. Dev Cell. 7: 855-69. PMID: 15572128

订购信息

产品名称产品编号规格价格数量收藏夹

RhoB 抗体 (C-5)

sc-8048
200 µg/ml
$316.00

RhoB (C-5): m-IgG1 BP-HRP 套装

sc-531631
200 µg Ab; 20 µg BP
$354.00

RhoB 抗体 (C-5) AC

sc-8048 AC
500 µg/ml, 25% agarose
$416.00

RhoB 抗体 (C-5) HRP

sc-8048 HRP
200 µg/ml
$316.00

RhoB 抗体 (C-5) FITC

sc-8048 FITC
200 µg/ml
$330.00

RhoB 抗体 (C-5) PE

sc-8048 PE
200 µg/ml
$343.00

RhoB 抗体 (C-5) Alexa Fluor® 488

sc-8048 AF488
200 µg/ml
$357.00

RhoB 抗体 (C-5) Alexa Fluor® 546

sc-8048 AF546
200 µg/ml
$357.00

RhoB 抗体 (C-5) Alexa Fluor® 594

sc-8048 AF594
200 µg/ml
$357.00

RhoB 抗体 (C-5) Alexa Fluor® 647

sc-8048 AF647
200 µg/ml
$357.00

RhoB 抗体 (C-5) Alexa Fluor® 680

sc-8048 AF680
200 µg/ml
$357.00

RhoB 抗体 (C-5) Alexa Fluor® 790

sc-8048 AF790
200 µg/ml
$357.00

RhoB (C-5) 中和勝肽

sc-8048 P
100 µg/0.5 ml
$68.00

What is your suggested secondary antibody for use in WB with this mouse monoclonal Rho B Antibody (C-5): sc-8048?

Asked by: Anna99
We suggest using one our Mouse IgG Binding Proteins as a secondary detection reagent, such as sc-516102. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 1
Date published: 2017-01-16
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Rated 1 out of 5 by from No signal in Western blot on ECNo bands in human IPSC-derived brain microvascular endothelial cell. Tested first with 1/1000 ON, also 1/500, still nothing.
Date published: 2022-03-04
Rated 5 out of 5 by from Good!Excellent results by WB with Rho B Antibody (C-5).
Date published: 2017-09-15
Rated 5 out of 5 by from sharp bandsThis antibody was tested on human aortic endothelial cells post various treatments. It gives specific band with no background. A non-specific was detected around 17 kDa
Date published: 2017-09-14
Rated 5 out of 5 by from This has worked well in WBThis antibody worked great on western blot on human NIH/3T3 cells cells.
Date published: 2017-08-16
Rated 1 out of 5 by from no signal in westernused this with several mouse and human breast cancer cell lines.I started with 1:1000 ,ON incubation , since no signal was detected , then used 1:200, ON incubation. still no signal. waste of time!
Date published: 2017-02-27
Rated 5 out of 5 by from Good Rho B AntibodyRho B (C-5) works well for Western blot (WB) and IHC(P). Strong band at 25 kDa.
Date published: 2016-12-21
Rated 5 out of 5 by from One publication discusses the use of product scOne publication discusses the use of product sc-8048 in Kinase Assays using Caco-2 and NIH/3T3 cells. -SCBT Publication Review
Date published: 2015-05-01
Rated 5 out of 5 by from Good data was obtained by Western Blot usingGood data was obtained by Western Blot using rat samples: A10 vascular smooth muscle cells, cardiomyocytes, hippocampi embryo and adult brain tissues. -SCBT Publication Review
Date published: 2015-04-17
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RhoB 抗体 (C-5) is rated 4.4 out of 5 by 14.
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