Rho B抗体 (C-5) 推荐用于 mouse, rat 和 human 来源的Rho B p21 WB, IP, IF, IHC(P) 和 ELISA检测

Rho B 抗体 (C-5): sc-8048

Rho B抗体 (C-5) is rated 4.7 out of 5 by 13.
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说明书

    • 抗-Rho B 抗体 C-5 是小鼠单克隆 IgG1 (kappa轻链),Rho B抗体,规格为200 µg/ml
    • 特异性抗原位于human物种的Rho B的内部区域内的氨基酸123-137之间
    • 抗-Rho B 抗体 (C-5) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Rho B p21; 也和以下物种反应,包括: 和 equine, canine, bovine and porcine
    • 抗-Rho B 抗体 (C-5) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 封闭肽,sc-8048 P
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)
    • 关于如何获取Rho B (C-5): sc-8048免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。

What is your suggested secondary antibody for use in WB with this mouse monoclonal Rho B Antibody (C-5): sc-8048?

Asked by: Anna99
We suggest using one our Mouse IgG Binding Proteins as a secondary detection reagent, such as sc-516102. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 1
Date published: 2017-01-16
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Rated 5 out of 5 by from Good! Excellent results by WB with Rho B Antibody (C-5).
Date published: 2017-09-15
Rated 5 out of 5 by from sharp bands This antibody was tested on human aortic endothelial cells post various treatments. It gives specific band with no background. A non-specific was detected around 17 kDa
Date published: 2017-09-14
Rated 5 out of 5 by from This has worked well in WB This antibody worked great on western blot on human NIH/3T3 cells cells.
Date published: 2017-08-16
Rated 1 out of 5 by from no signal in western used this with several mouse and human breast cancer cell lines.I started with 1:1000 ,ON incubation , since no signal was detected , then used 1:200, ON incubation. still no signal. waste of time!
Date published: 2017-02-27
Rated 5 out of 5 by from Good Rho B Antibody Rho B (C-5) works well for Western blot (WB) and IHC(P). Strong band at 25 kDa.
Date published: 2016-12-21
Rated 5 out of 5 by from One publication discusses the use of product sc One publication discusses the use of product sc-8048 in Kinase Assays using Caco-2 and NIH/3T3 cells. -SCBT Publication Review
Date published: 2015-05-01
Rated 5 out of 5 by from Good data was obtained by Western Blot using Good data was obtained by Western Blot using rat samples: A10 vascular smooth muscle cells, cardiomyocytes, hippocampi embryo and adult brain tissues. -SCBT Publication Review
Date published: 2015-04-17
Rated 5 out of 5 by from Cytoplasmic staining of cells in molecular Cytoplasmic staining of cells in molecular layer and Purkinje cells via immunoperoxidase staining of formalin fixed, paraffin embedded human cerebellum tissue. -SCBT QC
Date published: 2015-03-10
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