Date published: 2025-9-6
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
RhoA Antibody (26C4) - Western Blotting - Image 390867
Rho A 抗体 (26C4) Alexa Fluor® 647: sc-418 AF647. HL-60 (A), RAW 264. 7 (B) 和 C6 (C) 全细胞裂解液中 Rho A 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 用 Cruz Marker MW Tag-Alexa Fluor® 790 检测 Cruz Marker™ 分子量标准: sc-516731.
Rho A 抗体 (26C4) Alexa Fluor® 647: sc-418 AF647. HL-60 (A), RAW 264. 7 (B) 和 C6 (C) 全细胞裂解液中 Rho A 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 用 Cruz Marker MW Tag-Alexa Fluor® 790 检测 Cruz Marker™ 分子量标准: sc-516731.

RhoA 抗体 (26C4): sc-418

4.6(72)
寫評論提問

说明书
  • RhoA 抗体 26C4 是小鼠单克隆 IgG1 κ，RhoA抗体, 在1,309篇文献中引用，规格为200 µg/ml
  • 免疫human物种的的氨基酸120-150
  • RhoA 抗体 (26C4) 推荐用于 WB, IP, IF, IHC(P) 和 FCM，检测mouse, rat 和human 来源的 Rho A; 也和以下物种反应，包括： 和 equine, bovine, porcine and canine
  • 抗RhoA抗体（26C4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联生物素 用于WB, IHC(P) 和 ELISA；偶联TRITC 用于IF, IHC(P) 和 FCM
  • 关于如何获取RhoA (26C4): sc-418的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgGκ BP-HRP 是 RhoA 抗体 (26C4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与RhoA 抗体 (26C4) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Rho 抗体 包括 Rho 8, Rho A, Rho B, Rho C, Rho D, Rho G, Rho GAP p190-B, Rho J, Rho T1和Rho V

Rho A 抗体（26C4）是一种小鼠单克隆 IgG1 kappa 轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和流式细胞术（FCM）检测小鼠、大鼠和人类来源的 Rho A。Rho A (26C4) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合型。Rho A在调节肌动蛋白细胞骨架方面发挥着关键作用，而肌动蛋白细胞骨架对于细胞形态、运动和分裂等细胞过程至关重要。Rho A的正常功能对于维持细胞完整性和促进细胞间交流至关重要，特别是在响应外部信号时。Rho A失调与多种疾病有关，包括癌症，其中信号通路的改变会导致细胞迁移和侵袭增加。因此，了解Rho A的相互作用和调节机制对于开发肿瘤学和其他领域的靶向疗法至关重要。Rho A及其家族成员具有一个羧基末端CAAX结构域，该结构域对于翻译后修饰和膜定位至关重要，进一步强调了Rho A在细胞动力学和信号通路中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

RhoA 抗体 (26C4) 参考文献:

  1. p21rho 蛋白的翻译后修饰。  |  Adamson, P., et al. 1992. J Biol Chem. 267: 20033-8. PMID: 1400319
  2. 成年小鼠组织中与 ras 相关的 ralA, rho12 和 rab 基因的表达。  |  Olofsson, B., et al. 1988. Oncogene. 3: 231-4. PMID: 2457862
  3. 鉴定 ras/R-ras 和 rho 调控蛋白的不同细胞质靶标。  |  Garrett, MD., et al. 1989. J Biol Chem. 264: 10-3. PMID: 2491843
  4. 在没有磷酸肌醇水解的情况下，用 ras 蛋白对瑞士 3T3 细胞进行刮擦加载可迅速激活蛋白激酶 C。  |  Morris, JD., et al. 1989. Oncogene. 4: 27-31. PMID: 2536916
  5. Ras 从未涉足的领域：VPS35 引导 N-Ras 穿过细胞质。  |  Zhou, M. and Philips, MR. 2019. Small GTPases. 10: 20-25. PMID: 28129035
  6. 睾丸生殖细胞肿瘤中的K-RAS和N-RAS突变。  |  Hacioglu, BM., et al. 2017. Bosn J Basic Med Sci. 17: 159-163. PMID: 28426398
  7. ras 超家族蛋白。  |  Chardin, P. 1988. Biochimie. 70: 865-8. PMID: 3145021
  8. ras 基因。  |  Barbacid, M. 1987. Annu Rev Biochem. 56: 779-827. PMID: 3304147
  9. 胰腺癌中的突变 K-Ras：对野生型 N-Ras 和 K-Ras 依赖性细胞周期调控作用的深入分析。  |  Ferguson, R., et al. 2023. Curr Issues Mol Biol. 45: 2505-2520. PMID: 36975534
  10. 人类 rho cDNA 克隆 12 的核苷酸序列。  |  Yeramian, P., et al. 1987. Nucleic Acids Res. 15: 1869. PMID: 3822842
  11. 一个新的ras相关基因家族  |  Madaule, P. and Axel, R. 1985. Cell. 41: 31-40. PMID: 3888408

订购信息

产品名称产品编号规格价格数量收藏夹

RhoA 抗体 (26C4)

sc-418
200 µg/ml
$316.00

RhoA (26C4): m-IgG Fc BP-HRP 套装

sc-528109
200 µg Ab; 10 µg BP
$354.00

RhoA (26C4): m-IgGκ BP-HRP 套装

sc-520407
200 µg Ab, 40 µg BP
$354.00

RhoA 抗体 (26C4) AC

sc-418 AC
500 µg/ml, 25% agarose
$416.00

RhoA 抗体 (26C4) HRP

sc-418 HRP
200 µg/ml
$316.00

RhoA 抗体 (26C4) FITC

sc-418 FITC
200 µg/ml
$330.00

RhoA 抗体 (26C4) PE

sc-418 PE
200 µg/ml
$343.00

RhoA 抗体 (26C4) Alexa Fluor® 488

sc-418 AF488
200 µg/ml
$357.00

RhoA 抗体 (26C4) Alexa Fluor® 546

sc-418 AF546
200 µg/ml
$357.00

RhoA 抗体 (26C4) Alexa Fluor® 594

sc-418 AF594
200 µg/ml
$357.00

RhoA 抗体 (26C4) Alexa Fluor® 647

sc-418 AF647
200 µg/ml
$357.00

RhoA 抗体 (26C4) Alexa Fluor® 680

sc-418 AF680
200 µg/ml
$357.00

RhoA 抗体 (26C4) Alexa Fluor® 790

sc-418 AF790
200 µg/ml
$357.00

RhoA 抗体 (26C4) B

sc-418 B
200 µg/ml
$320.00

RhoA 抗体 (26C4) TRITC

sc-418 TRITC
200 µg/ml
$320.00

Hi, is this antibody capable to distinguish the active RhoA (RhoA-GTP) and the inactive form?

Asked by: kikyo
Thank you for your question. Please contact your local distributor or technical service for further assistance.
Answered by: Dallas Tech Service
Date published: 2021-03-16

Hi, what is the clone of rhoA antibody (sc-418)??

Asked by: Hala
Thank you for your question. The clone that produces this antibody is 26C4. It is based on an immunogen consisting of amino acids 120-150 of Rho A of human origin. Please contact our technical service for further information.
Answered by: Tech Support Europe
Date published: 2019-05-08

Hello, Could you tell me please if I can use Rho A Antibody (26C4): sc-418 with Opal 4-color IHC Kit (PerkinElmer), please? Secondly, is this antibody/sc-418 labelled/conjugates with any fluorophore? Many thanks. Kind regards, Sami

Asked by: Mamand
Thank you for your question. We have not specifically tested this antibody using the Opal 4-color IHC Kit from Perkin Elmer, however, based on their website unlabeled antibodies may be selected based on antibody performance. For more information, we suggest you inquire with that vendor. This mouse monoclonal, Rho A (26C4): sc-418, has been validated for IHC on formalin-fixed paraffin-embedded tissue sections so it is suitable for this research application. We do provide this primary antibody directly conjugated to HRP (sc-418 HRP), as well as several fluorophores, including: phycoerythrin (sc-418 PE), fluorescein (sc-418 FITC), Alexa Fluor® 488 (sc-418 AF488), Alexa Fluor® 647 (sc-418 AF647), TRITC (sc-418 TRITC) or Alexa Fluor® 405 (sc-418 AF405).
Answered by: Technical Support
Date published: 2017-01-23

How can this mouse monoclonal antibody be used for immunohistochemical staining of mouse tissue?

Asked by: Anonymous
We recommend using a directly conjugated mouse monoclonal primary antibody. This mouse monoclonal antibody Rho A (26C4) is currently available conjugated to AlexaFluor 405 (sc-418 AF405), AlexaFluor 488 (sc-418 AF488), AlexaFluor 647 (sc-418 AF647), FITC (sc-418 FITC), HRP (sc-418 HRP), PE (sc-418 PE), and TRITC (sc-418 TRITC).
Answered by: Tech Service 6
Date published: 2016-12-15

What is the recommended secondary reagent for Western blot using this mouse monoclonal primary antibody?

Asked by: Anonymous
We recommend our Mouse IgG Binding Protein conjugated to HRP as a secondary detection reagent: https://www.scbt.com/scbt/product/m-igg-kappa-bp-hrp A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 6
Date published: 2016-12-15
  • y_2025, m_8, d_27, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_5
  • loc_zh_CN, sid_418, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 99ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from Good for WBWork fine in western blot analysis in A498 ccRCC cells
Date published: 2024-11-26
Rated 4 out of 5 by from Good for WBWork fine in western blot analysis in A498 ccRCC cells
Date published: 2024-11-26
Rated 4 out of 5 by from Great for WBWorks great for western blot in hRECs and human RCC cells.
Date published: 2022-06-08
Rated 5 out of 5 by from WB RhoAUsed for detection by immunoblot of human RhoA: very sensitive, no background
Date published: 2021-12-02
Rated 4 out of 5 by from ¡Buen resultado!Compré este anticuerpo el mes pasado y funciona perfectamente para western blot. Se ven unas bandas muy limpias y sin ninguna inespecifidad.
Date published: 2021-11-10
Rated 5 out of 5 by from Great antibody!It works very well in western blot and immunofluorescence
Date published: 2021-10-08
Rated 4 out of 5 by from Good result in western blotI detected one single band and no background in western blot at a dilution of 1:1000 for mouse endothelial cells.
Date published: 2020-11-09
Rated 5 out of 5 by from 功能不错！一个月前定的抗体，用的小样，做免疫染色，效果还是挺可以的.......其他实验的效果暂时还没检测....
Date published: 2020-10-15
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_72
  • loc_zh_CN, sid_418, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 16ms
  • REVIEWS, PRODUCT
RhoA 抗体 (26C4) is rated 4.6 out of 5 by 72.
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_72
  • loc_zh_CN, sid_418, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 104ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。