Rev-erbβ Antibody (D-8): sc-398252
- Rev-erbβ Antibody (D-8) is a mouse monoclonal IgG1 κ Rev-erbβ antibody, cited in 5 publications, provided at 200 µg/ml
- raised against amino acids 195-267 mapping within an internal region of Rev-erbβ of mouse origin
- Rev-erb beta Antibody (D-8) is recommended for detection of Rev-erbβ of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Rev-erb beta Antibody (D-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-398252 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Rev-erbβ Antibody (D-8) for WB applications. These reagents are now offered in bundles with Rev-erbβ Antibody (D-8) (see ordering information below).
Rev-erbβ Antibody (D-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects Rev-erb beta (Rev-erbβ) protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Rev-erbβ (D-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Rev-erbβ protein plays a crucial role in regulating circadian rhythms and metabolic processes by acting as a transcriptional repressor. Rev-erbβ helps maintain the balance of gene expression related to the body′s internal clock, influencing sleep-wake cycles and energy homeostasis. Rev-erbβ interacts with specific DNA sequences, such as 5′-AATGTAGGTCA-3′ and 5′-ATAACTAGGTCA-3′, to inhibit retinoic acid-related orphan receptor alpha (RORα) activity, thereby modulating target gene expression involved in these critical biological processes. Both Rev-erbα and Rev-erbβ are classified as nuclear proteins within the nuclear hormone receptor family, highlighting their significance in cellular signaling and gene regulation.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Rev-erbβ Antibody (D-8) References:
- CIA, a novel estrogen receptor coactivator with a bifunctional nuclear receptor interacting determinant. | Sauvé, F., et al. 2001. Mol Cell Biol. 21: 343-53. PMID: 11113208
- Circadian expression of clock genes and clock-controlled genes in the rat retina. | Kamphuis, W., et al. 2005. Biochem Biophys Res Commun. 330: 18-26. PMID: 15781226
- Structural insight into the constitutive repression function of the nuclear receptor Rev-erbbeta. | Woo, EJ., et al. 2007. J Mol Biol. 373: 735-44. PMID: 17870090
- The orphan nuclear receptor Rev-erbbeta recruits Tip60 and HDAC1 to regulate apolipoprotein CIII promoter. | Wang, J., et al. 2008. Biochim Biophys Acta. 1783: 224-36. PMID: 17996965
- Identification of heme as the ligand for the orphan nuclear receptors REV-ERBalpha and REV-ERBbeta. | Raghuram, S., et al. 2007. Nat Struct Mol Biol. 14: 1207-13. PMID: 18037887
- Genomic organization of the human thyroid hormone receptor alpha (c-erbA-1) gene. | Laudet, V., et al. 1991. Nucleic Acids Res. 19: 1105-12. PMID: 1850510
- Dual inhibition of REV-ERBβ and autophagy as a novel pharmacological approach to induce cytotoxicity in cancer cells. | De Mei, C., et al. 2015. Oncogene. 34: 2597-608. PMID: 25023698
- Involvement of Nuclear Receptor REV-ERBβ in Formation of Neurites and Proliferation of Cultured Adult Neural Stem Cells. | Shimozaki, K. 2018. Cell Mol Neurobiol. 38: 1051-1065. PMID: 29397477
- A new orphan member of the nuclear hormone receptor superfamily closely related to Rev-Erb. | Dumas, B., et al. 1994. Mol Endocrinol. 8: 996-1005. PMID: 7997240
- Localization of the Rev-ErbA orphan receptors in the brain. | Kainu, T., et al. 1996. Brain Res. 743: 315-9. PMID: 9017260
- Structural elements of an orphan nuclear receptor-DNA complex. | Zhao, Q., et al. 1998. Mol Cell. 1: 849-61. PMID: 9660968
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rev-erbβ Antibody (D-8) | sc-398252 | 200 µg/ml | $322.00 | |||
Rev-erbβ Antibody (D-8): m-IgG Fc BP-HRP Bundle | sc-527756 | 200 µg Ab; 10 µg BP | $361.00 | |||
Rev-erbβ Antibody (D-8): m-IgG1 BP-HRP Bundle | sc-533129 | 200 µg Ab; 20 µg BP | $361.00 | |||
Rev-erbβ Antibody (D-8) X | sc-398252 X | 200 µg/0.1 ml | $322.00 | |||
Rev-erbβ Antibody (D-8) AC | sc-398252 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Rev-erbβ Antibody (D-8) HRP | sc-398252 HRP | 200 µg/ml | $322.00 | |||
Rev-erbβ Antibody (D-8) FITC | sc-398252 FITC | 200 µg/ml | $336.00 | |||
Rev-erbβ Antibody (D-8) PE | sc-398252 PE | 200 µg/ml | $349.00 | |||
Rev-erbβ Antibody (D-8) Alexa Fluor® 488 | sc-398252 AF488 | 200 µg/ml | $364.00 | |||
Rev-erbβ Antibody (D-8) Alexa Fluor® 546 | sc-398252 AF546 | 200 µg/ml | $364.00 | |||
Rev-erbβ Antibody (D-8) Alexa Fluor® 594 | sc-398252 AF594 | 200 µg/ml | $364.00 | |||
Rev-erbβ Antibody (D-8) Alexa Fluor® 647 | sc-398252 AF647 | 200 µg/ml | $364.00 | |||
Rev-erbβ Antibody (D-8) Alexa Fluor® 680 | sc-398252 AF680 | 200 µg/ml | $364.00 | |||
Rev-erbβ Antibody (D-8) Alexa Fluor® 790 | sc-398252 AF790 | 200 µg/ml | $364.00 |