Date published: 2025-9-6

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RCK 抗体 (E-12): sc-376433

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说明书
  • RCK 抗体 E-12 是小鼠单克隆 IgG2b κ,RCK抗体, 在15篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的RCK的N-terminus的氨基酸3-31之间
  • RCK 抗体 (E-12) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 RCK
  • 抗RCK抗体(E-12)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取RCK (E-12): sc-376433免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP是RCK Antibody (E-12) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现可与RCK Antibody (E-12) 搭配使用(请参阅下面的订购信息)。有关其它 m-IgGκ BP 结合物,请参阅我们完整的小鼠 IgG 结合蛋白列表。

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関連項目

RCK抗体(E-12)是一种IgG2b κ小鼠单克隆RCK抗体(也称为DEAD-Box解旋酶6抗体、DEAD/H(Asp-Glu-Ala-Asp/His)Box多肽6(RNA解旋酶54kD)抗体、DEAD(Asp-Glu-Ala-Asp)Box解旋酶6抗体、ATP依赖性RNA解旋酶P54抗体、DEAD Box蛋白6抗体、癌基因RCK抗体、Rck/P54抗体、HLR2抗体、IDDILF抗体、DDX6或P54抗体),可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类的RCK蛋白。RCK抗体(E-12)既可作为非共轭抗RCK抗体形式提供,也可作为多种共轭形式的抗RCK抗体提供,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。RCK,也称为DDX6和P54,是DEAD-box RNA解旋酶蛋白家族的成员,这些蛋白都具有共同的蛋白基序。RCK存在于大多数组织中,是一种具有ATP依赖性RNA解旋活性的解旋酶,同时也具有在5'-3'方向上降解RNA的能力。在非恶性细胞中,RCK与正常RNA代谢的所有过程相关,包括剪接、输出和翻译起始。编码RCK的基因发生突变可导致蛋白过度表达,从而改变其功能,使其成为正向调控细胞生长和增殖相关基因表达的癌基因。据信,RCK的主要功能是通过其解旋酶活性下调mRNA表达并维持细胞内正常的转录水平。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

RCK 抗体 (E-12) 参考文献:

  1. 在人类神经母细胞瘤细胞系中,两个死盒基因(DDX1 和 DDX6)的表达与 MYCN 的表达无关。  |  Akiyama, K., et al. 1999. Biochem Mol Biol Int. 47: 563-8. PMID: 10319407
  2. RNA 螺旋酶 p54(DDX6)是一种穿梭蛋白,参与了储存的 mRNP 粒子的核组装。  |  Smillie, DA. and Sommerville, J. 2002. J Cell Sci. 115: 395-407. PMID: 11839790
  3. 肿瘤缔合作用 DEAD-box 蛋白 rck/p54 在体外对 c-myc RNA 具有 RNA 解旋活性。  |  Akao, Y., et al. 2003. Genes Cells. 8: 671-6. PMID: 12875652
  4. rck/p54(一种 DEAD-box RNA 螺旋酶)在小鼠配子发生和早期胚胎发生过程中的表达。  |  Matsumoto, K., et al. 2005. Dev Dyn. 233: 1149-56. PMID: 15906376
  5. 人类 DEAD-box 蛋白 rck/p54 的结构洞察其底物识别与构象变化。  |  Matsui, T., et al. 2006. Genes Cells. 11: 439-52. PMID: 16611246
  6. 在人体细胞中,microRNA 诱导的基因沉默对翻译的抑制需要 RCK/p54。  |  Chu, CY. and Rana, TM. 2006. PLoS Biol. 4: e210. PMID: 16756390
  7. 人类 DEAD-box/RNA 解旋酶 rck/p54 通过影响培养细胞的细胞周期来维持细胞生长。  |  Akao, Y., et al. 2006. Int J Oncol. 29: 41-8. PMID: 16773183
  8. 爪蟾Xp54和人类RCK/p54螺旋酶在铬花叶病毒RNA复制中取代了酵母Dhh1p的功能。  |  Alves-Rodrigues, I., et al. 2007. J Virol. 81: 4378-80. PMID: 17301158
  9. Rck/p54 作为多蛋白复合物的一部分与 APP mRNA 相互作用,并增强 APP mRNA 和蛋白在神经细胞系中的表达。  |  Broytman, O., et al. 2009. Neurobiol Aging. 30: 1962-74. PMID: 18378046
  10. DDX6(Rck/p54)是丙型肝炎病毒高效复制所必需的,但不是内部核糖体进入位点定向翻译所必需的。  |  Jangra, RK., et al. 2010. J Virol. 84: 6810-24. PMID: 20392846
  11. AU-富集元素依赖性翻译抑制需要 tristetraprolin 和 RCK/P54 的合作。  |  Qi, MY., et al. 2012. Mol Cell Biol. 32: 913-28. PMID: 22203041
  12. rck/p54 候选原癌基因产物是一种 54 千道尔顿的 D-E-A-D 盒蛋白,在人类和小鼠组织中表达不同。  |  Akao, Y., et al. 1995. Cancer Res. 55: 3444-9. PMID: 7614484

订购信息

产品名称产品编号规格价格数量收藏夹

RCK 抗体 (E-12)

sc-376433
200 µg/ml
$316.00

RCK (E-12): m-IgGκ BP-HRP 套装

sc-522954
200 µg Ab, 40 µg BP
$354.00

RCK 抗体 (E-12) AC

sc-376433 AC
500 µg/ml, 25% agarose
$416.00

RCK 抗体 (E-12) HRP

sc-376433 HRP
200 µg/ml
$316.00

RCK 抗体 (E-12) FITC

sc-376433 FITC
200 µg/ml
$330.00

RCK 抗体 (E-12) PE

sc-376433 PE
200 µg/ml
$343.00

RCK 抗体 (E-12) Alexa Fluor® 488

sc-376433 AF488
200 µg/ml
$357.00

RCK 抗体 (E-12) Alexa Fluor® 546

sc-376433 AF546
200 µg/ml
$357.00

RCK 抗体 (E-12) Alexa Fluor® 594

sc-376433 AF594
200 µg/ml
$357.00

RCK 抗体 (E-12) Alexa Fluor® 647

sc-376433 AF647
200 µg/ml
$357.00

RCK 抗体 (E-12) Alexa Fluor® 680

sc-376433 AF680
200 µg/ml
$357.00

RCK 抗体 (E-12) Alexa Fluor® 790

sc-376433 AF790
200 µg/ml
$357.00

RCK (E-12) 中和勝肽

sc-376433 P
100 µg/0.5 ml
$68.00

What application is the blocking peptide sc-376433 P appropriate for?

Asked by: TinTin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Very goodThis antibody works well in western blot of human whole cell extracts.
Date published: 2021-05-31
Rated 5 out of 5 by from Great antibody!This antibody produced very specific and strong signals on WB (1:200, 5% milk).
Date published: 2018-12-24
Rated 5 out of 5 by from Great for IF!!!I used this antibody for IF. The antibody worked very well and showed specific staining!
Date published: 2017-10-31
Rated 5 out of 5 by from Great antibody for western blotI got this antibody and then tried it for western blot with 1:1000 dilution. It works really well: clear band, correct size, and low background.
Date published: 2017-08-14
Rated 5 out of 5 by from Excellent antibody!The RCK Antibody (E-12): sc-376433 works very well for Western blot applications, showing clear band with minimal background.
Date published: 2017-01-24
Rated 5 out of 5 by from Excellent immunofluorescence PExcellent immunofluorescence P-bodies and cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-01-02
Rated 5 out of 5 by from Nice Western Blot data of RCK expression in nonNice Western Blot data of RCK expression in non-transfected 293T, human RCK transfected 293T and K-562 whole cell lysates. -SCBT QC
Date published: 2014-06-24
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RCK 抗体 (E-12) is rated 5.0 out of 5 by 7.
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