RBC Lysis Buffer, 10X A high quality reagent supplied as a 10X solution, yields a quantity sufficient to lyse up to 500 samples. pH 7.1-7.4.

RBC Lysis Buffer, 10X

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应用; A high quality reagent supplied as a 10X solution, yields a quantity sufficient to lyse up to 500 samples. pH 7.1-7.4
* 参考分析证明 大量特定数据 (包括水 含量).
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Red Blood Cell (RBC) Lysis Buffer has been designed, formulated, and tested to ensure optimal lysis of RBCs in single cell suspensions with minimal effects on leukocytes.

RBC Lysis Buffer is supplied as a 10X solution containing ammonium chloride, potassium carbonate, and EDTA, and should be diluted in deionized water prior to use.


参考文献

1. Adams, R.A., et al. 2007. J. Exp. Med. 204: 571-582. PMID: 17339406
2. Field-Corbett, C., et al. 2009. Stem Cells. 27: 662-669. PMID: 19096034

用法 :
RBC lysis buffer is supplied as a 10X solution and should be diluted to 1X in deionized water. 100 ml of 10X concentrate will yield a quantity of 1X solution that is sufficient to lyse 500 samples.



The pH of the 1X solution should fall within the range of pH 7.1-7.4. Adjust the pH if necessary. Warm the 1X solution to room temperature prior to use.



NOTE: The blood sample must be <1 month old and been stored in anticoagulants (i.e. EDTA, heparin, and citrate).



PROTOCOL

1. Add whole blood to RBC Lysis Buffer at a ratio of approximately 1 part blood to 2 parts Lysis Buffer. Invert the tube to mix and incubate for 5 minutes at room temperature on a shaking platform or with periodic inversions. Do not vortex.

2. Centrifuge for 5 minutes then remove supernatant carefully without disturbing the pellet.


Note: If pellet is not white, repeat steps 1 and 2 with additional RBC Lysis Buffer.

3. If a lower red pellet is still visible below the white pellet then repeat steps 1 and 2 with fresh blood with the following modifications: a. Ensure the RBC Lysis Buffer is at room temperature. b. Increase the incubation to 15 minutes c. Use a higher ratio of RBC Lysis Buffer to blood (3:1).
规划 :
10X
物理状态 :
Liquid
溶解度 :
Soluble in water.
pH :
7.1-7.4
保存 :
Store at 4° C
仅供科研使用。不可用于诊断或治疗。

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分析证明书

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PMID: # 30530991  Pai, CS.|Simons, DM.|Lu, X.|Evans, M.|Wei, J.|Wang, YH.|Chen, M.|Huang, J.|Park, C.|Chang, A.|Wang, J.|Westmoreland, S.|Beam, C.|Banach, D.|Bowley, D.|Dong, F.|Seagal, J.|Ritacco, W.|Richardson, PL.|Mitra, S.|Lynch, G.|Bousquet, P.|Mankovich, J.|Kingsbury, G.|Fong, L.| et al. 2019. J. Clin. Invest. 129: 349-363.

PMID: # 30581133  Li, YJ.|Du, L.|Wang, J.|Vega, R.|Lee, TD.|Miao, Y.|Aldana-Masangkay, G.|Samuels, ER.|Li, B.|Ouyang, SX.|Colayco, SA.|Bobkova, EV.|Divlianska, DB.|Sergienko, E.|Chung, TDY.|Fakih, M.|Chen, Y.| et al. 2019. Cell Chem Biol. 26: 278-288.e6.

PMID: # 30737146  Pai, CS.|Huang, JT.|Lu, X.|Simons, DM.|Park, C.|Chang, A.|Tamaki, W.|Liu, E.|Roybal, KT.|Seagal, J.|Chen, M.|Hagihara, K.|Wei, XX.|DuPage, M.|Kwek, SS.|Oh, DY.|Daud, A.|Tsai, KK.|Wu, C.|Zhang, L.|Fasso, M.|Sachidanandam, R.|Jayaprakash, A.|Lin, I.|Casbon, AJ.|Kinsbury, GA.|Fong, L.| et al. 2019. Immunity. 50: 477-492.e8.

PMID: # 30560697  Rigg, RA. et al. 2019. Platelets. 30: 126-135.

PMID: # 30635395  Lyon de Ana, C.|Arakcheeva, K.|Agnihotri, P.|Derosia, N.|Winandy, S.| et al. 2019. J. Immunol. 202: 1112-1123.

PMID: # 29288204  Steinbuck, MP. et al. 2018. J. Immunol. 200: 997-1007.

PMID: # 29890544  Pagesy, P.|Tachet, C.|Mostefa-Kara, A.|Larger, E.|Issad, T.| et al. 2018. Exp. Clin. Endocrinol. Diabetes.

PMID: # 29781585  Cerquone Perpetuini, A.|Re Cecconi, AD.|Chiappa, M.|Martinelli, GB.|Fuoco, C.|Desiderio, G.|Castagnoli, L.|Gargioli, C.|Piccirillo, R.|Cesareni, G.| et al. 2018. J Cachexia Sarcopenia Muscle. 9: 727-746.

PMID: # 28024082  Browning, DL.|Everson, EM.|Leap, DJ.|Hocum, JD.|Wang, H.|Stamatoyannopoulos, G.|Trobridge, GD.| et al. 2017. Gene Ther. 24: 187-198.

PMID: # 26773739  Spada, F. et al. 2016. N Biotechnol.

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