Date published: 2025-9-6

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RANTES 抗体 (F-11): sc-514019

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说明书
  • RANTES 抗体 F-11 是小鼠单克隆 IgG1 κ,RANTES抗体, 在5篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的RANTES的C-terminus的氨基酸24-91之间
  • RANTES 抗体 (F-11) 推荐用于WB, IP, IF 和 ELISA,检测human 来源的 RANTES
  • 抗RANTES抗体(F-11)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取RANTES (F-11): sc-514019免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP是RANTES Antibody (F-11) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与RANTES Antibody (F-11) 搭配使用(请参阅下面的订购信息)。

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関連項目

RANTES抗体(F-11)是一种IgG1 κ小鼠单克隆RANTES抗体(也称为C-C基序趋化因子配体5抗体、SIS-Delta抗体、TCP228抗体、嗜酸粒细胞趋化因子抗体、趋化因子(C-C基序)配体5抗体、t细胞特异性蛋白P288抗体、β -趋化因子RANTES抗体、C-C基序趋化因子5抗体、SCYA5抗体、SISd抗体、EoCP抗体、小可诱导细胞因子A5 (RANTES)抗体、t细胞特异性RANTES蛋白抗体、T细胞特异性蛋白P228或小诱导细胞因子A5抗体),通过WB, IP, IF和ELISA检测人源性RANTES蛋白。RANTES抗体(F-11)可以作为非偶联的抗RANTES抗体形式,以及多种偶联的抗RANTES抗体形式,包括琼脂糖,HRP, PE, FITC和多个Alexa Fluor®偶联物。在结构上,C-C或β-趋化因子的特征是一组保守的相邻半胱氨酸。该家族成员包括MCP-1、MCP-2、MCP-3、MIP-1α、MIP-1β、RANTES和I-309。RANTES(受活化调节,正常T细胞表达和分泌)在血小板、嗜酸性粒细胞、成纤维细胞、巨噬细胞、内皮细胞和T淋巴细胞中表达。与趋化因子家族一致,RANTES对单核细胞和NK细胞具有较强的趋化活性。I-309最初被鉴定为存在于γ/δ T淋巴细胞的因子。I-309 cDNA编码一个长度为73个氨基酸的蛋白质,具有一个潜在的N -连接糖基化位点。与其他C-C家族成员不同,I-309不诱导自然杀伤(NK)细胞的趋化。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

RANTES 抗体 (F-11) 参考文献:

  1. RANTES 趋化因子启动子的多态性影响 HIV-1 疾病的进展。  |  Liu, H., et al. 1999. Proc Natl Acad Sci U S A. 96: 4581-5. PMID: 10200305
  2. RANTES 可增强抗原特异性粘膜免疫反应。  |  Lillard, JW., et al. 2001. J Immunol. 166: 162-9. PMID: 11123289
  3. RANTES 与人类原代巨噬细胞表达的辛迪加-1 和辛迪加-4 的相互作用。  |  Slimani, H., et al. 2003. Biochim Biophys Acta. 1617: 80-8. PMID: 14637022
  4. RANTES 启动子多态性上调与特应性皮炎有关。  |  Tanaka, K., et al. 2006. Int J Immunogenet. 33: 423-8. PMID: 17117952
  5. 利用可生物降解纳米颗粒靶向输送 Psc-RANTES 以预防 HIV-1。  |  Ham, AS., et al. 2009. Pharm Res. 26: 502-11. PMID: 19002569
  6. CCL5/RANTES 是器官培养中退行性椎间盘释放的一种关键趋化吸引因子。  |  Pattappa, G., et al. 2014. Eur Cell Mater. 27: 124-36; discussion 136. PMID: 24500793
  7. CCL5/RANTES 在慢性肝病期间对 HCC 进展的功能性作用。  |  Mohs, A., et al. 2017. J Hepatol. 66: 743-753. PMID: 28011329
  8. 鉴定 CCL5/RANTES 为小鼠骨骼肌中一种新型收缩还原性肌动因。  |  Ishiuchi, Y., et al. 2018. Cytokine. 108: 17-23. PMID: 29558694
  9. 鼻腔上皮细胞和内皮细胞产生的 RANTES。  |  Terada, N., et al. 1996. J Allergy Clin Immunol. 98: S230-7. PMID: 8977532
  10. CSF-1、MCP-1/JE和RANTES在成功妊娠期间巨噬细胞募集中的相对作用。  |  Wood, GW., et al. 1997. Mol Reprod Dev. 46: 62-9; discussion 69-70. PMID: 8981365

订购信息

产品名称产品编号规格价格数量收藏夹

RANTES 抗体 (F-11)

sc-514019
200 µg/ml
$316.00

RANTES (F-11): m-IgG Fc BP-HRP 套装

sc-526341
200 µg Ab; 10 µg BP
$354.00

RANTES 抗体 (F-11) AC

sc-514019 AC
500 µg/ml, 25% agarose
$416.00

RANTES 抗体 (F-11) HRP

sc-514019 HRP
200 µg/ml
$316.00

RANTES 抗体 (F-11) FITC

sc-514019 FITC
200 µg/ml
$330.00

RANTES 抗体 (F-11) PE

sc-514019 PE
200 µg/ml
$343.00

RANTES 抗体 (F-11) Alexa Fluor® 488

sc-514019 AF488
200 µg/ml
$357.00

RANTES 抗体 (F-11) Alexa Fluor® 546

sc-514019 AF546
200 µg/ml
$357.00

RANTES 抗体 (F-11) Alexa Fluor® 594

sc-514019 AF594
200 µg/ml
$357.00

RANTES 抗体 (F-11) Alexa Fluor® 647

sc-514019 AF647
200 µg/ml
$357.00

RANTES 抗体 (F-11) Alexa Fluor® 680

sc-514019 AF680
200 µg/ml
$357.00

RANTES 抗体 (F-11) Alexa Fluor® 790

sc-514019 AF790
200 µg/ml
$357.00

As described below question, I also got a band only above 25kDa instead of 8kDa on cell lysate. Is the 25kDa band derived from RANTES?

Asked by: watanabe
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

I ran 20ug of cell lysates from Beas2b and probed with this antibody in the ratio 1:1000 but I got a band only above 25kDa instead of 8kDa. But in your data sheet the band shows 6kDa.Will this antibody detects any precursor? What would u suggest me to do?

Asked by: karti
Thank you for your question. We are not aware of any form of RANTES that would be expected to be observed at a molecular weight above 25 kDa. We would recommend increasing both the amount of protein you are loading, as well as the concentration of primary antibody. For the data shown on our website, we load 50 µg of human platelet extract, and the primary antibody was used at a dilution of 1:200. A link to our recommended Western blotting protocol with this antibody is provided below. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting If you still do not observe the expected size of RANTES, please contact our Technical Service Department and we can assist you with troubleshooting the antibody.
Answered by: Technical Support
Date published: 2017-03-09

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with RANTES (F-11): sc-514019?

Asked by: Cweed
We recommend using one of our our exclusive Mouse IgG Binding Proteins conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support
Date published: 2017-02-27
  • y_2025, m_8, d_30, h_9CST
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Rated 5 out of 5 by from The antibody is perfectI got this antibody two months ago, it is perfect.
Date published: 2021-11-30
Rated 5 out of 5 by from Great for western blotWork well in WB at 1:100, WPMY-1 cells. Strip of rantese is exact.
Date published: 2017-07-14
Rated 1 out of 5 by from Not good antibody as expectedI received this sample antibody from santacruz, but it did not work for me. I used 1: 1000 and 1:500 dilution of antibody and checked them on Beas2b and NHBE cell lysate. But I couldn't see a band less than 10kDa but Im getting a single nice band around 25kDa.
Date published: 2017-03-20
Rated 5 out of 5 by from perfectStrip clear, background clean,WB data of rantese is exact.
Date published: 2016-11-03
Rated 5 out of 5 by from Great Western blot data of RANTES expressionGreat Western blot data of RANTES expression in human platelet extract. -SCBT QC
Date published: 2013-06-23
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RANTES 抗体 (F-11) is rated 4.2 out of 5 by 5.
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