Date published: 2025-9-10

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Rad51 抗体 (G-9): sc-377467

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说明书
  • Rad51抗体(G-9)是小鼠单克隆IgG1 κ, 在15篇文献中引用,规格为200 µg/ml
  • 免疫human物种的Rad51的氨基酸1-92
  • 抗-Rad51 抗体 (G-9) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Rad51
  • 抗Rad51抗体(G-9)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Rad51 (G-9): sc-377467免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP 是 Rad51 抗体 (G-9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与Rad51 抗体 (G-9) 组合成套装提供。 (请参阅下面的订购信息)。其他m-IgGκ BP 偶联产品,请参阅我们的小鼠 IgG 结合蛋白完整列表。

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関連項目

Rad51抗体(G-9)是一种IgG1 κ小鼠单克隆Rad51抗体,它能够通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类的Rad51蛋白。Rad51抗体(G-9)有非偶联的抗Rad51抗体形式,也有多种偶联形式的抗Rad51抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Rad52家族成员(Rad50、Rad51B/C/D、Rad52、Rad54、MRE11)介导DNA双链断裂修复(DSBR),以修复可能导致细胞死亡、突变或肿瘤转化的DNA损伤。Rad51(RECA,BRCC5)与BRCA1和BRCA2相互作用,影响亚细胞定位和细胞对DNA损伤的反应。BRCA2的失活可能是导致基因组不稳定和Rad51失调引发肿瘤发生的关键事件。Rad52形成一个七聚体环,该环与单链DNA末端结合,并催化互补链退火所需的DNA-DNA相互作用。Rad52可以与Rad51相互作用。DEAD样解旋酶超家族的Rad54A与双链DNA结合并诱导DNA拓扑结构变化,这被认为有助于同源DNA配对并刺激DNA重组。DEAD样解旋酶超家族的Rad54B与双链DNA结合,并在DNA存在下显示ATP酶活性。Rad54B在睾丸和脾脏中含量丰富,该基因的突变发生在原发性淋巴瘤和结肠癌中。MRE11(减数分裂重组11,ATLD,HNGS1)是一种核3'-5'核酸外切酶/内切酶,与Rad50相关,影响同源重组、端粒长度维持和DNA双链断裂修复。MRE11在增殖组织中含量最为丰富。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Rad51 抗体 (G-9)

sc-377467
200 µg/ml
$316.00

Rad51 (G-9): m-IgGκ BP-HRP 套装

sc-523339
200 µg Ab, 40 µg BP
$354.00

Rad51 抗体 (G-9) AC

sc-377467 AC
500 µg/ml, 25% agarose
$416.00

Rad51 抗体 (G-9) HRP

sc-377467 HRP
200 µg/ml
$316.00

Rad51 抗体 (G-9) FITC

sc-377467 FITC
200 µg/ml
$330.00

Rad51 抗体 (G-9) PE

sc-377467 PE
200 µg/ml
$343.00

Rad51 抗体 (G-9) Alexa Fluor® 488

sc-377467 AF488
200 µg/ml
$357.00

Rad51 抗体 (G-9) Alexa Fluor® 546

sc-377467 AF546
200 µg/ml
$357.00

Rad51 抗体 (G-9) Alexa Fluor® 594

sc-377467 AF594
200 µg/ml
$357.00

Rad51 抗体 (G-9) Alexa Fluor® 647

sc-377467 AF647
200 µg/ml
$357.00

Rad51 抗体 (G-9) Alexa Fluor® 680

sc-377467 AF680
200 µg/ml
$357.00

Rad51 抗体 (G-9) Alexa Fluor® 790

sc-377467 AF790
200 µg/ml
$357.00

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 15
Date published: 2017-02-07
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Rated 4 out of 5 by from It works well in WB!We used it at 1:100 diluition in BSA and it works very well.
Date published: 2020-10-13
Rated 5 out of 5 by from Great signalWe Used for WB at 1:100 dilution, with human infected B lymphocytes and in Panc1 cells and had a great signal
Date published: 2020-02-10
Rated 1 out of 5 by from not recomended for WBno signal for WB (dilution range 1:50 - 1:1000 in BSA/TBS-T or MMP/TBS-T) and only a very weak signal in IF (dilution 1:50) if the cells were fixed in 2% formaldehyde. Even then the background signal is too high to obtain a good signal.
Date published: 2020-01-09
Rated 4 out of 5 by from Good antibody for WBUsed 1:500 5% milk TBST overnight at 4 degrees. Worked very nicely.
Date published: 2018-03-05
Rated 1 out of 5 by from Not useful for IFTried to detect rad51 foci after DNA damage in cell line. It does not work, very few signal, nucleus barely stained (dilution 1/500)
Date published: 2018-02-07
Rated 5 out of 5 by from Great antibody!Used for WB at 1:1000 dilution, i worked with rat cell line. See clean and strong bands between 35 and 55 kDa markers.
Date published: 2017-11-16
Rated 5 out of 5 by from Great!Worked well for my rat cell line. Saw a clear band between 35 and 55kDa marker.
Date published: 2017-11-15
Rated 5 out of 5 by from Excellent choice!This mouse monoclonal antibody, clone (G-9), has been validated for detection of Rad51 of mouse, rat and human origin in Western blot and IHC(P) research applications. An excellent choice!
Date published: 2017-01-24
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Rad51 抗体 (G-9) is rated 4.0 out of 5 by 12.
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