Date published: 2025-9-7

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Rab 5 抗体 (D-11): sc-46692

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说明书
  • Rab 5 抗体 D-11 是小鼠单克隆 IgG2b κ,Rab 5抗体, 在112篇文献中引用,规格为200 µg/ml
  • 与氨基酸1-215结合,代表全长Rab 5AA,源自human
  • Rab 5 抗体 (D-11) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Rab 5A, 5B and 5C; partially cross reactive with other Rab family members; 也和以下物种反应,包括: canine
  • 抗Rab 5抗体(D-11)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Rab 5 (D-11): sc-46692免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP2b BP-HRP">m-IgG2b BP-HRPm-IgGκ BP-HRP是Rab 5 Antibody (D-11) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与Rab 5 Antibody (D-11) 打包提供(请参阅下面的订购信息)。

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関連項目

Rab 5 抗体(D-11)是一种小鼠单克隆 IgG2b kappa 轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、免疫组织化学和酶联免疫吸附测定(ELISA)检测小鼠、大鼠和人类来源的 Rab 5 蛋白。Rab 5 (D-11) 抗体有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。Rab 5蛋白在调节细胞内吞和蛋白质运输方面发挥着关键作用,特别是将载体囊泡从内质网运送到高尔基体,并最终运送到分泌囊泡。这一过程维持细胞内稳态,促进不同细胞区室之间的沟通。Rab 5与各种效应蛋白相互作用,这些效应蛋白对于囊泡对接和融合至关重要,确保蛋白质被输送到正确目的地。Rab 5的正常运作至关重要,因为其活性紊乱会导致各种疾病,包括神经退行性疾病和癌症,这凸显了基础研究和临床研究的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Rab 5 抗体 (D-11) 参考文献:

  1. 细胞内运输中的 GTP 结合蛋白。  |  Pfeffer, SR. 1992. Trends Cell Biol. 2: 41-6. PMID: 14731525
  2. 通过 PCR 克隆方法揭示 Rab 和 Rho GTP 结合蛋白亚家族的复杂性。  |  Chavrier, P., et al. 1992. Gene. 112: 261-4. PMID: 1555775
  3. 克隆主要在脂肪细胞中表达的 Rab3 同工型。  |  Baldini, G., et al. 1992. Proc Natl Acad Sci U S A. 89: 5049-52. PMID: 1594612
  4. 登革热和西尼罗河病毒的细胞进入需要Rab 5。  |  Krishnan, MN., et al. 2007. J Virol. 81: 4881-5. PMID: 17301152
  5. RAB-5和DYNAMIN-1介导的EFF-1融合蛋白的内吞作用控制细胞-细胞融合。  |  Smurova, K. and Podbilewicz, B. 2016. Cell Rep. 14: 1517-1527. PMID: 26854231
  6. LET-413/Erbin在胞吞循环过程中充当RAB-5效应子,促进RAB-10激活。  |  Liu, H., et al. 2018. J Cell Biol. 217: 299-314. PMID: 29079669
  7. 通过表位标签对 Rab8 和 Rab10 这两种低分子量 GTP 结合蛋白进行表达和定域。  |  Chen, YT., et al. 1993. Proc Natl Acad Sci U S A. 90: 6508-12. PMID: 7688123
  8. Coatomers 和 SNAREs 在促进膜交通方面的作用。  |  Takizawa, PA. and Malhotra, V. 1993. Cell. 75: 593-6. PMID: 8242734
  9. 朋友和家人:Rab GTPases 在囊泡交通中的作用。  |  Novick, P. and Brennwald, P. 1993. Cell. 75: 597-601. PMID: 8242735
  10. GTP 结合蛋白在外排途径运输中的作用。  |  Ferro-Novick, S. and Novick, P. 1993. Annu Rev Cell Biol. 9: 575-99. PMID: 8280472
  11. 血小板聚集过程中低分子量 GTP 结合蛋白 rap2B 与细胞骨架的关联  |  Torti, M., et al. 1993. Proc Natl Acad Sci U S A. 90: 7553-7. PMID: 8356055
  12. 鉴定人类血小板中的小 GTP 结合型 rab 蛋白:凝血酶诱导的 rab3B, rab6 和 rab8 蛋白磷酸化。  |  Karniguian, A., et al. 1993. Proc Natl Acad Sci U S A. 90: 7647-51. PMID: 8356066

订购信息

产品名称产品编号规格价格数量收藏夹

Rab 5 抗体 (D-11)

sc-46692
200 µg/ml
$316.00

Rab 5 (D-11): m-IgG Fc BP-HRP 套装

sc-528501
200 µg Ab; 10 µg BP
$354.00

Rab 5 (D-11): m-IgGκ BP-HRP 套装

sc-520893
200 µg Ab, 40 µg BP
$354.00

Rab 5 (D-11): m-IgG2b BP-HRP 套装

sc-550046
200 µg Ab; 10 µg BP
$354.00

Rab 5 抗体 (D-11) AC

sc-46692 AC
500 µg/ml, 25% agarose
$416.00

Rab 5 抗体 (D-11) HRP

sc-46692 HRP
200 µg/ml
$316.00

Rab 5 抗体 (D-11) FITC

sc-46692 FITC
200 µg/ml
$330.00

Rab 5 抗体 (D-11) PE

sc-46692 PE
200 µg/ml
$343.00

Rab 5 抗体 (D-11) Alexa Fluor® 488

sc-46692 AF488
200 µg/ml
$357.00

Rab 5 抗体 (D-11) Alexa Fluor® 546

sc-46692 AF546
200 µg/ml
$357.00

Rab 5 抗体 (D-11) Alexa Fluor® 594

sc-46692 AF594
200 µg/ml
$357.00

Rab 5 抗体 (D-11) Alexa Fluor® 647

sc-46692 AF647
200 µg/ml
$357.00

Rab 5 抗体 (D-11) Alexa Fluor® 680

sc-46692 AF680
200 µg/ml
$357.00

Rab 5 抗体 (D-11) Alexa Fluor® 790

sc-46692 AF790
200 µg/ml
$357.00

I see that the Rab 5 Antibody (D-11) is reactive with multiple species. Will it work with bovine samples?

Asked by: Trav11
To check additional species reactivity not listed on our website, please contact our Technical Services department at 800-457-3801 and we will be more than happy to run sequence analysis for your species of interest.
Answered by: Tech Service 8
Date published: 2016-12-20

Is it possible to use the Rab 5 Antibody (D-11) for double staining if my other primary antibody was also raised in mice?

Asked by: Trav11
Yes, it is possible. For cases like this, we recommend using our directly conjugated mouse monoclonal primary antibodies. Currently, the Rab 5 Antibody (D-11): sc-46692 is available conjugated to HRP (sc-46692 HRP), phycoerythrin (sc-46692 PE), fluoroscein (sc-46692 FITC), Alexa Fluor® 488 (sc-46692 AF488) or Alexa Fluor® 647 (sc-46692 AF647).
Answered by: Tech Service 8
Date published: 2016-12-20
  • y_2025, m_8, d_27, h_5CST
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Rated 5 out of 5 by from Lavora molto bene in WBQuesto anticorpo, inviato come sample, lavora molto bene in WB con diluizione 1:1000, su lisati di cellule PC3.
Date published: 2023-05-04
Rated 5 out of 5 by from Works very well in Western and IFWorks well in Western blot and immunofluorescence.
Date published: 2021-01-13
Rated 5 out of 5 by from great for wbI got the sample size and it worked great in the WB.
Date published: 2020-07-08
Rated 5 out of 5 by from excellentOne clear crisp band, predicted molecular weights using concentration of 1:1000
Date published: 2020-02-06
Rated 5 out of 5 by from Nice staining in IF for early endosomewe tested Rab 5 with IF in the cell lines and worked quite well at 1:500 4 C overnight and second AB RT 1h.
Date published: 2018-05-17
Rated 3 out of 5 by from WBRab 5 (D-11) did not worked in the WB of mouse lung primary fibroblast. We used anti-mouse IgG as the secondary antibody. Maybe the problem was the secondary antibody.
Date published: 2018-03-28
Rated 1 out of 5 by from 免疫染色固定した繊維芽細胞の免疫染色に使用しました。通常通り1:400に希釈して使用しましたが良い染色性は得られま戦でした。
Date published: 2018-03-22
Rated 5 out of 5 by from Good antibody for WBOne band detected at right size. Dilution used: 1/1000. Block with 2% BSA
Date published: 2018-03-07
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Rab 5 抗体 (D-11) is rated 4.4 out of 5 by 35.
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