QUIN 2, Tetrapotassium Salt CAS: 73630-23-6
MF: C26H23N3O10 4K
MW: 693.9
A useful fluorescing calcium indicator.

QUIN 2, Tetrapotassium Salt (CAS 73630-23-6)

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应用; A useful fluorescing calcium indicator
CAS号码: 73630-23-6
分子量: 693.9
分子式: C26H23N3O10 4K
* 参考分析证明 大量特定数据 (包括水 含量).
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QUIN 2, Tetrapotassium Salt has been demonstrated to be an indicator of Zn2+-protein complexes.The binding of Ca2+ to QUIN 2 leads to a major shift in UV absorption spectrum and a twenty-fold enhancement in fluorescence quantum yield. TPEN (sc-200131) can be used in conjunction with QUIN 2 to reduce heavy-metal quenching of QUIN 2 fluorescence in cells. Spectral properties in the presence of low calcium concentrations (ex. 354 nm, em. 510 nm) differ from those during high calcium conditions (ex. 332 nm, em. 505 nm).


参考文献

1. Komada, H., et al. 1989. Cell Struct. Funct. 14: 141. PMID: 2743418
2. Vijayaraghavan, S., et al. 1989. Cell Calcium 10: 241-253. PMID: 2776189
3. Arslan, P., et al. 1985. J. Biol. Chem. 260: 2719-2727. PMID: 3919006
4. Bryant, D.T. 1985. Biochem. J. 226: 613-616. PMID: 3994676
5. Grynkiewicz, G., et al. 1985. J. Biol. Chem. 260: 3440-3450. PMID: 3838314
6. Ashley R.H., et al. 1984. Biochem. J. 219: 149-158. PMID: 6326754
Tsien, R.Y., et al. 1982. J. Cell Biol. 94: 325-334. PMID: 6980885
Tsien, R.Y., et al. 1980. Biochemistry. 19: 2396-2404. PMID: 6770893

物理状态 :
Solid
溶解度 :
Soluble in water (10 mg/ml).
保存 :
Store at -20° C
熔点 :
163.9-166.1° C
沸点 :
831.6° C at 760 mmHg (Predicted)
荧光 :
λex 332 nm, λem 493 nm in 0.1 M Tris pH 8.0, 10 mM Ca2+; λex 332-354 nm, λem 505-510 nm
仅供科研使用。不可用于诊断或治疗。
德国水公害等级 :
3
PubChem CID :
135539
默克索引 :
14: 8042
MDL 号码 :
MFCD00065487
Beilstein 注册 :
5374262
SMILES :
CC1=CC(=C(C=C1)N(CC(=O)[O-])CC(=O)[O-])OCC2=NC3=C(C=C(C=C3C=C2)OC)N(CC(=O)[O-])CC(=O)[O-].[K+].[K+].[K+].[K+]

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