Date published: 2025-9-6
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PSD-95 Antibody (7E3) - Western Blotting - Image 372314
PSD-95 抗体 (7E3) HRP: sc-32290 HRP. 小鼠大脑 (A), 大鼠大脑 (B) 和大鼠海马 (C) 组织提取物中 PSD-95 表达的直接 Western 印迹分析.
PSD-95 Antibody (7E3) - Western Blotting - Image 372314
PSD-95 抗体 (7E3) HRP: sc-32290 HRP. 小鼠大脑 (A), 大鼠大脑 (B) 和大鼠海马 (C) 组织提取物中 PSD-95 表达的直接 Western 印迹分析.
PSD-95 Antibody (7E3) - Western Blotting - Image 10418
PSD-95 抗体 (7E3): sc-32290. 小鼠大脑 (A) 和大鼠大脑 (B) 组织提取物中 PSD-95 表达的 Western 印迹分析.
PSD-95 Antibody (7E3) - Western Blotting - Image 373442
PSD-95 抗体 (7E3): sc-32290. 小鼠大脑 (A), 大鼠海马 (B) 和人类大脑皮层 (C) 组织提取物中 PSD-95 表达的 Western 印迹分析.
PSD-95 抗体 (7E3) HRP: sc-32290 HRP. 小鼠大脑 (A), 大鼠大脑 (B) 和大鼠海马 (C) 组织提取物中 PSD-95 表达的直接 Western 印迹分析.

PSD-95 抗体 (7E3): sc-32290

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说明书
  • PSD-95 抗体 7E3 是小鼠单克隆 IgG1 κ，PSD-95抗体, 在106篇文献中引用，规格为200 µg/ml
  • 针对rat来源的纯化重组PSD-95
  • PSD-95 抗体 (7E3) 推荐用于 WB, IP 和 IF，检测mouse, rat 和human 来源的 PSD-95
  • 抗PSD-95抗体（7E3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取PSD-95 (7E3): sc-32290的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是PSD-95 Antibody (7E3) for WB applications. 的首选辅助检测试剂，这些试剂现与PSD-95 Antibody (7E3) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Post-Synaptic Density Protein抗体，包括PSD-95

PSD-95抗体（7E3）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）和免疫荧光（IF）检测小鼠、大鼠和人类来源的PSD-95蛋白。抗PSD-95抗体（7E3）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。PSD-95，也称为SAP 90，在组织突触信号复合物中起着至关重要的作用，对于兴奋性突触的正常功能至关重要。PSD-95主要位于神经元的突触后密度区，在那里PSD-95与各种受体和支架蛋白相互作用，促进谷氨酸受体和其他信号分子的聚集。这种定位对于突触可塑性至关重要，而突触可塑性是学习和记忆的基础。PSD-95与其他蛋白质（如NMDA和AMPA受体）形成复合物，凸显了PSD-95在调节突触强度和传递中的重要性。此外，PSD-95与肿瘤抑制蛋白APC等蛋白质的相互作用表明其在调节细胞生长和信号通路中的作用，突出了突触功能以外的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PSD-95 抗体 (7E3) 参考文献:

  1. 鸟苷酸激酶与其底物 GMP 复合物的精制结构，分辨率为 2.0 A。  |  Stehle, T. and Schulz, GE. 1992. J Mol Biol. 224: 1127-41. PMID: 1314905
  2. 大鼠大脑突触后密度部分含有果蝇圆盘大肿瘤抑制蛋白的同源物。  |  Cho, KO., et al. 1992. Neuron. 9: 929-42. PMID: 1419001
  3. 黑腹果蝇的肿瘤抑制基因。  |  Gateff, E. and Mechler, BM. 1989. Crit Rev Oncog. 1: 221-45. PMID: 2518684
  4. PSD-95 保护突触免受β-淀粉样蛋白的侵害。  |  Dore, K., et al. 2021. Cell Rep. 35: 109194. PMID: 34077732
  5. O-GlcNAc化调节SynGAP/PSD-95的液-液相分离。  |  Lv, P., et al. 2022. Nat Chem. 14: 831-840. PMID: 35637289
  6. 一种PSD-95肽类似物可减轻安格曼综合征小鼠模型的神经功能缺陷。  |  Lau, KA., et al. 2023. Prog Neurobiol. 230: 102513. PMID: 37536482
  7. 靶向突触后谷氨酸受体支架蛋白PSD-95和PICK1治疗肥胖症。  |  Fadahunsi, N., et al. 2024. Sci Adv. 10: eadg2636. PMID: 38427737
  8. 与 SAP90 和果蝇盘状大肿瘤抑制蛋白同源的新型突触前蛋白 SAP97 的分子特征和空间分布。  |  Müller, BM., et al. 1995. J Neurosci. 15: 2354-66. PMID: 7891172
  9. 克隆和鉴定 hdlg：果蝇盘状大肿瘤抑制因子与蛋白 4.1 结合的人类同源物。  |  Lue, RA., et al. 1994. Proc Natl Acad Sci U S A. 91: 9818-22. PMID: 7937897
  10. ZO-1, DlgA 和 PSD-95/SAP90：紧密, 隔膜和突触细胞连接中的同源蛋白。  |  Woods, DF. and Bryant, PJ. 1993. Mech Dev. 44: 85-9. PMID: 8155583
  11. APC 与果蝇盘状大肿瘤抑制蛋白的人类同源物的结合。  |  Matsumine, A., et al. 1996. Science. 272: 1020-3. PMID: 8638125
  12. NE-dlg的克隆与特征：果蝇圆盘大（dlg）肿瘤抑制蛋白与APC蛋白相互作用的新型人类同源物。  |  Makino, K., et al. 1997. Oncogene. 14: 2425-33. PMID: 9188857

订购信息

产品名称产品编号规格价格数量收藏夹

PSD-95 抗体 (7E3)

sc-32290
200 µg/ml
$316.00

PSD-95 (7E3): m-IgG Fc BP-HRP 套装

sc-526673
200 µg Ab; 10 µg BP
$354.00

PSD-95 (7E3): m-IgG1 BP-HRP 套装

sc-532046
200 µg Ab; 20 µg BP
$354.00

PSD-95 抗体 (7E3) AC

sc-32290 AC
500 µg/ml, 25% agarose
$416.00

PSD-95 抗体 (7E3) HRP

sc-32290 HRP
200 µg/ml
$316.00

PSD-95 抗体 (7E3) FITC

sc-32290 FITC
200 µg/ml
$330.00

PSD-95 抗体 (7E3) PE

sc-32290 PE
200 µg/ml
$343.00

PSD-95 抗体 (7E3) Alexa Fluor® 488

sc-32290 AF488
200 µg/ml
$357.00

PSD-95 抗体 (7E3) Alexa Fluor® 546

sc-32290 AF546
200 µg/ml
$357.00

PSD-95 抗体 (7E3) Alexa Fluor® 594

sc-32290 AF594
200 µg/ml
$357.00

PSD-95 抗体 (7E3) Alexa Fluor® 647

sc-32290 AF647
200 µg/ml
$357.00

PSD-95 抗体 (7E3) Alexa Fluor® 680

sc-32290 AF680
200 µg/ml
$357.00

PSD-95 抗体 (7E3) Alexa Fluor® 790

sc-32290 AF790
200 µg/ml
$357.00

I used the rat hippocampus protein sample and saw two bands with the Ab, as the picture shows. why?

Asked by: Cnida
Thank you for your question. They may be non-specific bands or isomers of the protein. We recommend reaching out to our Asian Technical Service department at Phone: (86 21) 6093-6350 or via e-mail at asia@scbio.cn Thank you.
Answered by: Ling Chen
Date published: 2024-11-05

What is the dilution for WB in tissue?

Asked by: Evelyn
Thank you for your question. PSD-95 (7E3) is recommended for detection of PSD-95 of mouse, rat and human origin by Western Blotting (starting dilution 1:200, dilution range 1:100-1:1000).
Answered by: Ling Chen
Date published: 2024-10-24

Please, which dilution for IF in cells?

Asked by: ClauMar
Thank you for your question. For immunofluorescence applications with PSD-95 Antibody (7E3): sc-32290 we recommend a dilution range of 1:50-1:500 with a starting dilution of 1:50.
Answered by: Tech Support Europe
Date published: 2020-12-16

This antiboby can be used for the immunohistochemistry-paraffin embedded tissue. (IHC-P)

Asked by: Pavan88
Thank you for your question. We have not yet tested this antibody, PSD-95 (7E3): sc-32290, on formalin-fixed paraffin-embedded tissue sections and so IHC(P) is not currently a recommended application. We do recommend this antibody for IF or immunofluorescent staining of fresh or frozen tissues or cells fixed in acetone or methanol.
Answered by: Technical Support
Date published: 2017-03-30

I want to know what secondary antibody of PSD-95 Antibody (7E3). Thank you

Asked by: Michelle Hsieh
Thank you for your question. PSD-95 Antibody (7E3) is a mouse monoclonal IgG1 with a kappa light chain. We recommend using one of our new Mouse IgG kappa Binding Proteins in place of a conventional secondary antibody. For example, for Western blotting we would recommend our HRP conjugated, m-IgGκ BP-HRP: sc-516102, (https://www.scbt.com/scbt/product/m-igg-kappa-bp-hrp). A complete list of Mouse IgG Binding Proteins is available on our website using the link below. https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-130b1ry Any conventional anti-mouse IgG secondary antibody would also work just fine with this primary antibody.
Answered by: Technical Support
Date published: 2017-02-13

What is the epitope recognized by PSD-95 Antibody (7E3): sc-32290?

Asked by: cjMara
PSD-95 Antibody (7E3) is a mouse monoclonal raised against purified recombinant PSD-95 of rat origin. The exact epitope has not been determined.
Answered by: Technical Support 13
Date published: 2017-01-09
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Rated 5 out of 5 by from Strong and consistent signalWe have been using this antibody for the past few years. It has been great for western blotting as well as immunostaining using dissociated neuronal cultures.
Date published: 2022-04-18
Rated 5 out of 5 by from 功能很好我们用来做WB挺多，挺好用的，条带很容易做出来，很好的试剂，可放心购入。其他的实验还没有做过~~~~
Date published: 2020-12-30
Rated 5 out of 5 by from Western blot of PSD-95 in rat brainSpecific band of Postsynaptic density protein 95 (PSD-95) Western blot expression in rat hippocampal brain tissue. Dilution 1:200.
Date published: 2019-08-13
Rated 4 out of 5 by from Good for IFThis PSD-95 Antibody (7E3), sc-32290, shows strong specific signal in IF staining in primary rat motor neuron.
Date published: 2018-02-20
Rated 5 out of 5 by from great experience5% bsa blocked, 1:750 primary antibody,1:5000 secondary antibody
Date published: 2018-01-18
Rated 4 out of 5 by from Ok.The blot bands can be detected at the right sites.
Date published: 2017-11-15
Rated 5 out of 5 by from PSD95 WESTERN BLOTI TRIED THIS ANTIBODY ON MOUSE BRAIN TISSUE FOR WESTERN BLOT. THE SIGNAL WAS VERY GOOD.
Date published: 2017-11-04
Rated 5 out of 5 by from Great for blotting neuronal lysatesWe received this antibody as a sample and blotted mature rat cortical neurons. The blots were clean and the signal was good when we used the IgG kappa chain -HRP antibody as the secondary. The results were less than optimal when we used a goat antimouse-IgG (H+L)-HRP secondary
Date published: 2017-10-05
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PSD-95 抗体 (7E3) is rated 4.8 out of 5 by 26.
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