Date published: 2026-10-16
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PKC alpha Antibody (H-7) - Western Blotting - Image 387579
同时对 MOLT-4 (A), Jurkat (B), HeLa (C) 和 NIH/3T3 (D) 全细胞裂解液中 PKC α 表达 (用 PKC α 抗体 (H-7) Alexa Fluor® 790: sc-8393 AF790 检测) 和 GAPDH 表达 (用 GAPDH (G-9) Alexa Fluor® 680: sc-365062 AF680 检测) 进行直接近红外 Western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断.
同时对 MOLT-4 (A), Jurkat (B), HeLa (C) 和 NIH/3T3 (D) 全细胞裂解液中 PKC α 表达 (用 PKC α 抗体 (H-7) Alexa Fluor® 790: sc-8393 AF790 检测) 和 GAPDH 表达 (用 GAPDH (G-9) Alexa Fluor® 680: sc-365062 AF680 检测) 进行直接近红外 Western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断.

PKC alpha 抗体 (H-7): sc-8393

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说明书
  • PKC alpha 抗体 H-7 是小鼠单克隆 IgG1 κ，PKC alpha抗体, 在324篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的PKC α的C-terminus的氨基酸651-672之间
  • PKC alpha 抗体 (H-7) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 PKC α
  • 抗PKC alpha抗体（H-7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是PKC alpha Antibody (H-7) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与PKC alpha Antibody (H-7) 打包提供（请参阅下面的订购信息）。
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研究信息

関連項目

额外PKC抗体，包括PKC α, PKC β, PKC δ, PKC ε, PKC η, PKC γ, PKC ι, PKC λ, PKC μ, PKC ν, PKC θ和PKC ζ

PKC α 抗体（H-7）是一种小鼠单克隆 IgG1 kappa 轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人源的 PKC α 蛋白。PKC α 抗体（H-7）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种 Alexa Fluor® 结合物。蛋白激酶C（PKC）家族成员之一PKCα蛋白在调节细胞生长、分化、基因表达和激素分泌等许多细胞功能方面发挥着至关重要的作用，因此PKCα对于维持细胞稳态至关重要。PKC alpha 主要位于细胞质中，激活后转移到细胞膜，这是 PKC alpha 在信号传导通路中发挥作用的关键。这种转移是由二酰基甘油（DAG）和肿瘤促进剂福尔博酯引发的，它们与 PKC alpha 结合并激活它，使 PKC alpha 能够磷酸化目标蛋白并调节各种细胞反应。PKC同工型在不同组织中的不同表达模式凸显了PKCα在特定信号通路中的重要性，而PKCα独特的辅助因子依赖性进一步强调了PKCα在PKC家族中独特的调节机制。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PKC alpha 抗体 (H-7) 参考文献:

  1. 蛋白激酶C-α（PKC-α）在黑色素瘤中的作用。  |  Lahn, MM. and Sundell, KL. 2004. Melanoma Res. 14: 85-9. PMID: 15057036
  2. 蛋白激酶 C 家族的新成员 nPKC theta 主要在骨骼肌中表达。  |  Osada, S., et al. 1992. Mol Cell Biol. 12: 3930-8. PMID: 1508194
  3. 白藜芦醇调节胃癌细胞中的细胞PKCα和δ，抑制其生长并诱导其凋亡。  |  Atten, MJ., et al. 2005. Invest New Drugs. 23: 111-9. PMID: 15744586
  4. 信号转导蛋白激酶家族（蛋白激酶 C 家族）的结构和功能多样性；两类不同的 PKC，即传统的 cPKC 和新型的 nPKC。  |  Ohno, S., et al. 1991. Adv Enzyme Regul. 31: 287-303. PMID: 1877391
  5. 蛋白激酶 C-delta 的表达和特征。  |  Olivier, AR. and Parker, PJ. 1991. Eur J Biochem. 200: 805-10. PMID: 1915352
  6. PKC-α调节TGF-β信号并影响足细胞存活。  |  Tossidou, I., et al. 2009. Cell Physiol Biochem. 24: 627-34. PMID: 19910703
  7. 2019年冠状病毒病（COVID-19）, 人类红细胞和PKC-α/β抑制剂白屈菜红碱（chelerythrine）--可能的治疗意义。  |  Ghashghaeinia, M., et al. 2020. Cell Cycle. 19: 3399-3405. PMID: 33305655
  8. 膜磷脂对多功能蛋白激酶的钙依赖性激活。  |  Takai, Y., et al. 1979. J Biol Chem. 254: 3692-5. PMID: 438153
  9. 肌醇磷脂的周转和信号转导  |  Nishizuka, Y. 1984. Science. 225: 1365-70. PMID: 6147898
  10. 蛋白激酶 C 在细胞表面信号转导和肿瘤促进中的作用。  |  Nishizuka, Y. Nature. 308: 693-8. PMID: 6232463
  11. 蛋白激酶 C 可能是促进肿瘤生长的植物醇酯的受体蛋白。  |  Kikkawa, U., et al. 1983. J Biol Chem. 258: 11442-5. PMID: 6311812
  12. 促进肿瘤生长的植物醇酯直接激活钙激活的磷脂依赖性蛋白激酶。  |  Castagna, M., et al. 1982. J Biol Chem. 257: 7847-51. PMID: 7085651

订购信息

产品名称产品编号规格价格数量收藏夹

PKC alpha 抗体 (H-7)

sc-8393
200 µg/ml
$322.00

PKC alpha (H-7): m-IgG Fc BP-HRP 套装

sc-528219
200 µg Ab; 10 µg BP
$361.00

PKC alpha (H-7): m-IgGκ BP-HRP 套装

sc-520544
200 µg Ab, 40 µg BP
$361.00

PKC alpha (H-7): m-IgG1 BP-HRP 套装

sc-542822
200 µg Ab; 20 µg BP
$361.00

PKC alpha 抗体 (H-7) AC

sc-8393 AC
500 µg/ml, 25% agarose
$424.00

PKC alpha 抗体 (H-7) HRP

sc-8393 HRP
200 µg/ml
$322.00

PKC alpha 抗体 (H-7) FITC

sc-8393 FITC
200 µg/ml
$336.00

PKC alpha 抗体 (H-7) PE

sc-8393 PE
200 µg/ml
$349.00

PKC alpha 抗体 (H-7) Alexa Fluor® 488

sc-8393 AF488
200 µg/ml
$364.00

PKC alpha 抗体 (H-7) Alexa Fluor® 546

sc-8393 AF546
200 µg/ml
$364.00

PKC alpha 抗体 (H-7) Alexa Fluor® 594

sc-8393 AF594
200 µg/ml
$364.00

PKC alpha 抗体 (H-7) Alexa Fluor® 647

sc-8393 AF647
200 µg/ml
$364.00

PKC alpha 抗体 (H-7) Alexa Fluor® 680

sc-8393 AF680
200 µg/ml
$364.00

PKC alpha 抗体 (H-7) Alexa Fluor® 790

sc-8393 AF790
200 µg/ml
$364.00

PKC alpha (H-7) 中和勝肽

sc-8393 P
100 µg/0.5 ml
$69.00

does this product work on zebrafish embryo?any suggestion for zebrafish use,the product SC-216 is not available now!

Asked by: liyana
Thank you for your question. We are not aware of any publications citing use of this mouse monoclonal antibody, PKC α (H-7): sc-8393, in zebrafish samples. The antibody is raised against a peptide that does share significant (76%) sequence identity with PKC α in zebrafish, so there is a good chance that it would work in this species. For a limited time, we are offering a free 10 µg sample of this antibody. Please contact our Technical Service Department if you would like to request a sample to test in zebrafish.
Answered by: Technical Support
Date published: 2017-07-18

What secondary detection reagent should be used with PKC α Antibody (H-7) for Western blot?

Asked by: Trav11
We recommend using one of our exclusive Mouse IgG Binding Proteins as a secondary detection reagent, such as m-IgGκ BP-HRP: sc-516102, for Western blot. A complete list of all available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 8
Date published: 2021-10-29
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Rated 5 out of 5 by Cxlxy from well function in WB and IFGood results when tested PKC alpha in western blot of whole cell extracts or IFfrom rat articular chondrocytes.
Date published: 2023-12-22
Rated 5 out of 5 by Pharmacology_IBK from Dendritic morphology of bipolar cells in wild type10 to 15 weeks old mice were anesthetized with isoflurane (Vetflurane®k, Virbac) and killed by cervical dislocation. Vertical sections: The following steps were conducted at room temperature if not stated otherwise. Eyes were quickly removed from the eye socket, opened at the scleral-corneal rim and immersed for 10 min with 4% paraformaldehyde (PFA) in 1X phosphate-buffered saline (1X PBS, pH 7.4). Cornea, lens and vitreous were removed. Eye cups were then fixed with 4%PFA/1X PBS for 20 min, washed four times with 1X PBS and cryoprotected by a graded sucrose series: 10% sucrose in 1X PBS for 1 h, 20% sucrose in 1X PBS for 1 h and 30% sucrose in 1X PBS overnight at 4 °C. Eyecups were orientated along the dorsoven-tral axis, embedded in OCT Medium (Tissue-Tek O.C.T Compound; Sakura Finetek, Tokyo, Japan) and frozen in liquid nitrogen. Vertical sections (16 μm) were cut on a cryostat (Leica Microsystems, Wetzlar, Germany), mounted on gelatine coated slides and stored at − 20 °C. For immunofluorescence experiments, sections were washed three times in 1X PBS-T (1X PBS + 0.1% Triton X-100, Sigma-Aldrich, St. Louis, MO, USA), blocked for 1 h in 1X PBS-T containing 1% bovine serum albumin (BSA, Sigma-Aldrich, A7030) and incubated overnight at 4 °C with primary antibodies diluted in 1X PBS-T at concentrations listed in Supplementary table 1. After wash-ing three times with 1X PBS-T, sections were incubated with the secondary antibodies (Supplementary table 2) for one. Additional washes preceded the counterstaining with DAPI (1:10,000; Sigma, D-9542) and eventually the sections were mounted using Poly/Mount (Polysciences, Inc., Warrington, PA, USA)
Date published: 2022-01-17
Rated 5 out of 5 by M_Ibk from Works great for IHC in mouse retinaWe use this antibody as standard marker in our lab. Very intense and nice staining of bipolar cells in the retina
Date published: 2022-01-05
Rated 5 out of 5 by deka from Works very good!Used this antibody for IF (1:50/1:100/1:200) on cryosections (10µm) from human retinal organoids and obtained nice images (for all dilutions)!
Date published: 2019-07-23
Rated 5 out of 5 by SSC Lab from Works great on pig retinaPKCa staining (IHC-Fr) on 8um thick pig retina for bipolar cells Blocking: 5% donkey serum P Ab: 1:50 (overnight) S Ab: anti-mouse 1:200 (1h)
Date published: 2017-10-23
Rated 5 out of 5 by Wester from Nice AntibodyPKC α Antibody gives very good signal and manipulating conveniently with good control effect.The clarity of result can be  too good for us.
Date published: 2017-10-18
Rated 5 out of 5 by Mae1 from Great productWe used this antibody for our western blot. give us good results.
Date published: 2017-09-27
Rated 5 out of 5 by stvphen from IHCThe relationship between the expression of PKC- alpha protein and the clinicopathological features of lung cancer was detected by IHC with this antibody.It is found that PKC-A is closely related to the development of lung cancer. We found PKA-A canbe used assit for lung cancer diagnosis.We recommend the use of 1:300 for immunohistochemistry.
Date published: 2017-09-16
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PKC alpha 抗体 (H-7) is rated 4.8 out of 5 by 34.
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