Date published: 2025-9-8

021-6093-6350

SCBT Portrait Logo
Seach Input

Pitx2 抗体 (H-1): sc-390457

3.0(6)
寫評論提問

说明书
  • Pitx2 抗体 H-1 是小鼠单克隆 IgG1 κ,Pitx2抗体, 在3篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的Pitx2B的C-terminus附近的氨基酸246-262之间
  • Pitx2 抗体 (H-1) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 Pitx2A, Pitx2B and Pitx2C
  • 抗Pitx2抗体(H-1)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于ChIP的TransCruz试剂(sc-390457 X, 200 µg/0.1 ml)
  • 关于如何获取Pitx2 (H-1): sc-390457免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 1 BP-HRP">m-IgG1 BP-HRP是Pitx2 Antibody (H-1) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与Pitx2 Antibody (H-1) 打包提供(请参阅下面的订购信息)。

快捷链接

関連項目

Pitx2抗体(H-1)是一种IgG1 κ小鼠单克隆Pitx2抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和酶联免疫吸附测定(ELISA)检测到源自小鼠、大鼠和人类的Pitx2蛋白。Pitx2抗体(H-1)既有非偶联形式的抗Pitx2抗体,也有多种偶联形式的抗Pitx2抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Pitx1和Pitx2是高度同源的、与bicoid相关的转录因子。Pitx1是一种与bicoid相关的同源结构域因子,在后肢中表现出优先表达,并在发育中的前垂体和第一鳃弓中也有表达。Pitx1位点的缺失导致后肢特异性标记物T-box因子(Tbx4)的远端表达减少。Pitx1可能调节特定后肢区域的形态发生、生长和模式形成,并作为影响前肢和后肢形态和生长差异的变量组分之一。Pitx2最初被确定为人类Rieger综合征的致病基因,这是一种导致发育异常的常染色体显性遗传病。Pitx2是一种转录因子,可调节心脏定位以及垂体和牙齿的形态发生。Pitx2还通过编码肺的"左倾性"来调节肺的对称性。Pitx2在左侧板中胚层中呈不对称表达,具有侧性缺陷的突变小鼠显示出Pitx2表达模式的改变,这与内脏对称性的变化相关。编码Pitx1和Pitx2的基因分别定位于人类染色体5q31和4q25-q26。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Pitx2 抗体 (H-1) 参考文献:

  1. Bicoid相关同源染色体因子Pitx1在后肢形态发生和垂体发育中的作用  |  Szeto, DP., et al. 1999. Genes Dev. 13: 484-94. PMID: 10049363
  2. 下丘脑-垂体轴的发育模型:转录下丘脑。  |  Kioussi, C., et al. 1999. Mech Dev. 81: 23-35. PMID: 10330482
  3. 肢体发育:告别武器  |  Graham, A. and McGonnell, I. 1999. Curr Biol. 9: R368-70. PMID: 10339420
  4. Pitx2调节肺的不对称性, 心脏定位以及垂体和牙齿的形态发生。  |  Lin, CR., et al. 1999. Nature. 401: 279-82. PMID: 10499586
  5. Xpitx-1:一种在爪蟾胚胎垂体和粘液腺形成过程中表达的同源染色体基因。  |  Hollemann, T. and Pieler, T. 1999. Mech Dev. 88: 249-52. PMID: 10534625
  6. 心房颤动和节律控制中 PITX2 依赖性基因调控。  |  Syeda, F., et al. 2017. J Physiol. 595: 4019-4026. PMID: 28217939
  7. 通过计算模型了解 PITX2 依赖性心房颤动机制  |  Bai, J., et al. 2021. Int J Mol Sci. 22: PMID: 34299303
  8. Pitx2 通过潜伏的 TGF&beta 形成一种加速--制动机械反馈模式,使肠道旋转。  |  Sanketi, BD., et al. 2022. Science. 377: eabl3921. PMID: 36137018
  9. PITX2 基因敲除诱发持续性心房颤动电重塑的关键发现  |  Schulz, C., et al. 2023. Circ Arrhythm Electrophysiol. 16: e011602. PMID: 36763906
  10. PITX2 诱导导致心律失常性心肌病的心肌细胞功能受损。  |  van Kampen, SJ., et al. 2023. Stem Cell Reports. 18: 749-764. PMID: 36868229
  11. 垂体同源染色体 2 是双核相关同源染色体家族的一个新成员,是前部结构形成的潜在调节因子。  |  Gage, PJ. and Camper, SA. 1997. Hum Mol Genet. 6: 457-64. PMID: 9147650
  12. 人类和鼠类的 PTX1/Ptx1 基因与特雷撤-科林斯综合征的病变区域有关。  |  Crawford, MJ., et al. 1997. Mamm Genome. 8: 841-5. PMID: 9337397

订购信息

产品名称产品编号规格价格数量收藏夹

Pitx2 抗体 (H-1)

sc-390457
200 µg/ml
$316.00

Pitx2 (H-1): m-IgG1 BP-HRP 套装

sc-531740
200 µg Ab; 20 µg BP
$354.00

Pitx2 抗体 (H-1) X

sc-390457 X
200 µg/0.1 ml
$316.00

Pitx2 抗体 (H-1) AC

sc-390457 AC
500 µg/ml, 25% agarose
$416.00

Pitx2 抗体 (H-1) HRP

sc-390457 HRP
200 µg/ml
$316.00

Pitx2 抗体 (H-1) FITC

sc-390457 FITC
200 µg/ml
$330.00

Pitx2 抗体 (H-1) PE

sc-390457 PE
200 µg/ml
$343.00

Pitx2 抗体 (H-1) Alexa Fluor® 488

sc-390457 AF488
200 µg/ml
$357.00

Pitx2 抗体 (H-1) Alexa Fluor® 546

sc-390457 AF546
200 µg/ml
$357.00

Pitx2 抗体 (H-1) Alexa Fluor® 594

sc-390457 AF594
200 µg/ml
$357.00

Pitx2 抗体 (H-1) Alexa Fluor® 647

sc-390457 AF647
200 µg/ml
$357.00

Pitx2 抗体 (H-1) Alexa Fluor® 680

sc-390457 AF680
200 µg/ml
$357.00

Pitx2 抗体 (H-1) Alexa Fluor® 790

sc-390457 AF790
200 µg/ml
$357.00

Pitx2 (H-1) 中和勝肽

sc-390457 P
100 µg/0.5 ml
$68.00

Protein was extracted from the whole eyeball of C57 mice for WB, two bands appeared between 55-75KDA. There was no band in 32KDA. What is going on?

Asked by: quanlity
Thank you for your question. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2019-08-19

Is this antibody work in chicken ?

Asked by: lava
Thank you for your question. Unfortunately this antibody is not chicken reactive.
Answered by: Technical Support
Date published: 2019-01-26

Was previous antibody worked with avian derived tissue. However, avaian is not listed for this monoclonal antibody. Was it not tested in avian tissue or did it not label avian tissue?

Asked by: BBjorke
Thank you for your question. We have not yet tested this mouse monoclonal antibody, Pitx2 (H-1): sc-390457, for reactivity with avian tissue samples.
Answered by: Technical Support
Date published: 2017-09-27

What application is the blocking peptide sc-390457 P appropriate for?

Asked by: Germaine
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
  • y_2025, m_9, d_7, h_4CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_4
  • loc_zh_CN, sid_390457, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 111ms
  • QUESTIONS, PRODUCT
Rated 1 out of 5 by from anti Pitx2 (H-1) AbThe anti Pitx2 mAb (H-1) showed broad, NON-specific reactivity in IF experimens on criosections of mouse embryonic and adult hearts. The hearts had been fixed 1h in PFA 1% on ice; the Ab has been incubated ON @+4C (dilution range 1:10-1:100), secondary Ab was anti mouse IgG 1:400.
Date published: 2020-10-05
Rated 1 out of 5 by from No reaction in IFI had very weak staining in immunofluorescence in neonatal mouse heart, with different procedures and dilutions, starting from 1:10 and up (lot number L2719). Very disappointing.
Date published: 2020-04-16
Rated 5 out of 5 by from Work well!1:200 diluted for wb of rat heart protein ,the band is clear
Date published: 2019-08-18
Rated 5 out of 5 by from positive western blotWe used this antibody in a western blot and produced positive band results at the correct molecular weight
Date published: 2017-01-25
Rated 1 out of 5 by from Multiple bands at wrong sizes in WB...Whole cell extract from hESC-derived cardiac progenitor cells. 35 ug proteins was blotted with Pitx2 ab (H-1) at 1:1000 dilution. Multiple bands were observed at ~48kD, 52kD, 60kD, and 120kD.
Date published: 2016-10-27
Rated 5 out of 5 by from Satisfactory Western blot data of Pitx2 expressionSatisfactory Western blot data of Pitx2 expression in Y79 nuclear extract. -SCBT QC
Date published: 2014-11-18
  • y_2025, m_9, d_7, h_4
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_6
  • loc_zh_CN, sid_390457, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 7ms
  • REVIEWS, PRODUCT
Pitx2 抗体 (H-1) is rated 3.0 out of 5 by 6.
  • y_2025, m_9, d_7, h_4
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_6
  • loc_zh_CN, sid_390457, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 103ms
  • REVIEWS, PRODUCT