Date published: 2025-9-8
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
Pim-2 Antibody (1D12) - Western Blotting - Image 403851
Pim-2 抗体 (1D12) HRP: sc-13514 HRP. 非转染: sc-117752 (A) 和人 Pim-2 转染: sc-111264 (B) 全细胞裂解液中 Pim-2 表达的直接 Western 印迹分析.
Pim-2 抗体 (1D12) HRP: sc-13514 HRP. 非转染: sc-117752 (A) 和人 Pim-2 转染: sc-111264 (B) 全细胞裂解液中 Pim-2 表达的直接 Western 印迹分析.

Pim-2 抗体 (1D12): sc-13514

4.9(11)
寫評論提問

说明书
  • Pim-2 抗体 1D12 是小鼠单克隆 IgG1 κ，Pim-2抗体, 在31篇文献中引用，规格为200 µg/ml
  • 针对全长Pim-2的mouse来源
  • Pim-2 抗体 (1D12) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 Pim-2
  • 抗Pim-2抗体（1D12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Pim-2 (1D12): sc-13514的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 Pim-2 抗体 (1D12) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Pim-2 抗体 (1D12) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Pim 抗体 包括 Pim-1, Pim-2和Pim-3

Pim-2抗体（1D12）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过WB、IP、IF和IHCP检测小鼠、大鼠和人源Pim-2。Pim-2 (1D12) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种 Alexa Fluor® 结合物。Pim-2，也称为莫洛尼鼠白血病病毒的病毒前体整合位点，是一种丝氨酸/苏氨酸激酶，通过促进细胞周期进展和抑制细胞凋亡，在细胞存活和增殖中发挥关键作用。这一功能在造血细胞中尤为重要，Pim-2参与造血调节和干细胞群维持。Pim-2已被证实可与多种蛋白质相互作用，包括促凋亡蛋白Bad，Pim-2通过磷酸化Bad来抑制其促凋亡活性，从而促进细胞在压力条件下的存活。在各种组织中，包括造血系、白血病和淋巴瘤细胞以及大脑中，都检测到了Pim-2的人类转录本，其中Pim-2与长期增强的形成和保存有关，而长期增强是长期记忆的关键过程。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Pim-2 抗体 (1D12) 参考文献:

  1. Pim 激酶的表达受 LTP 刺激的诱导，是巩固持久 LTP 的必要条件。  |  Konietzko, U., et al. 1999. EMBO J. 18: 3359-69. PMID: 10369676
  2. pim 激酶的发育表达表明，其功能也不局限于造血系统。  |  Eichmann, A., et al. 2000. Oncogene. 19: 1215-24. PMID: 10713710
  3. Pim-2 对淋巴细胞的转化依赖于核因子-kappaB 的激活。  |  Hammerman, PS., et al. 2004. Cancer Res. 64: 8341-8. PMID: 15548703
  4. Pim 和 Akt 致癌基因是造血细胞生长和存活的独立调节因子。  |  Hammerman, PS., et al. 2005. Blood. 105: 4477-83. PMID: 15705789
  5. 靶向丝氨酸/苏氨酸激酶 Pim-2 的反义寡脱氧核苷酸抑制了 DU-145 细胞的增殖。  |  Dai, JM., et al. 2005. Acta Pharmacol Sin. 26: 364-8. PMID: 15715935
  6. 将分子动力学模拟和分子力学/广义Born表面积计算整合到药效团建模中：莫洛尼小鼠白血病病毒前病毒整合位点激酶抑制剂的案例研究。  |  Gao, J., et al. 2020. J Biomol Struct Dyn. 38: 581-588. PMID: 30678548
  7. PIM1（莫洛尼小鼠白血病病毒前病毒整合位点）作为肺动脉高压的新型生物标志物和治疗靶点：癌症理论的又一证据。  |  Satoh, K., et al. 2020. Arterioscler Thromb Vasc Biol. 40: 500-502. PMID: 32101474
  8. B细胞特异性莫洛尼小鼠白血病病毒整合位点1敲除可抑制胃癌细胞的阿霉素耐药性。  |  Ma, N., et al. 2023. Arab J Gastroenterol. 24: 168-174. PMID: 36878814
  9. XBP1s-PIM-2正反馈回路控制IL-15介导的自然杀伤细胞存活。  |  Ma, S., et al. 2023. Sci Immunol. 8: eabn7993. PMID: 36897958
  10. 缺乏 Pim-1 原癌基因的 E mu-myc 转基因小鼠体内的病毒标记会导致 Pim-2 的代偿性激活。  |  van der Lugt, NM., et al. 1995. EMBO J. 14: 2536-44. PMID: 7781606
  11. Pim-2 转基因诱导淋巴肿瘤，并与 c-myc 产生强大的协同作用。  |  Allen, JD., et al. 1997. Oncogene. 15: 1133-41. PMID: 9294606
  12. 人类 Pim-2 原癌基因及其睾丸表达。  |  Baytel, D., et al. 1998. Biochim Biophys Acta. 1442: 274-85. PMID: 9804974

订购信息

产品名称产品编号规格价格数量收藏夹

Pim-2 抗体 (1D12)

sc-13514
200 µg/ml
$316.00

Pim-2 (1D12): m-IgG Fc BP-HRP 套装

sc-526550
200 µg Ab; 10 µg BP
$354.00

Pim-2 (1D12): m-IgG1 BP-HRP 套装

sc-531923
200 µg Ab; 20 µg BP
$354.00

Pim-2 抗体 (1D12) AC

sc-13514 AC
500 µg/ml, 25% agarose
$416.00

Pim-2 抗体 (1D12) HRP

sc-13514 HRP
200 µg/ml
$316.00

Pim-2 抗体 (1D12) FITC

sc-13514 FITC
200 µg/ml
$330.00

Pim-2 抗体 (1D12) PE

sc-13514 PE
200 µg/ml
$343.00

Pim-2 抗体 (1D12) Alexa Fluor® 488

sc-13514 AF488
200 µg/ml
$357.00

Pim-2 抗体 (1D12) Alexa Fluor® 546

sc-13514 AF546
200 µg/ml
$357.00

Pim-2 抗体 (1D12) Alexa Fluor® 594

sc-13514 AF594
200 µg/ml
$357.00

Pim-2 抗体 (1D12) Alexa Fluor® 647

sc-13514 AF647
200 µg/ml
$357.00

Pim-2 抗体 (1D12) Alexa Fluor® 680

sc-13514 AF680
200 µg/ml
$357.00

Pim-2 抗体 (1D12) Alexa Fluor® 790

sc-13514 AF790
200 µg/ml
$357.00

I would like to know if this conjugated Pim-2 (1D12): sc-13514 antibody is usable for flow cytometry application and gives reliable results? Thank you in advance.

Asked by: MarionH
Thank you for your question. The conjugated Pim-2 is recommended for flow cytometry applications. Note that we offer a warranty for our products for the recommended applications. If you need additional information, please contact our Technical Service.
Answered by: Technical Support Europe
Date published: 2018-03-29

Can Pim-2 (1D12): sc-13514 monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: jenniferc
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. Pim-2 (1D12): sc-13514 is available conjugated to agarose (sc-13514 AC), 500 µg/0.25 ml agarose in 1 ml, for IP; HRP (sc-13514 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; phycoerythrin (sc-13514 PE), fluorescein (sc-13514 FITC), Alexa Fluor® 488 (sc-13514 AF488) or Alexa Fluor® 647 (sc-13514 AF647), 200 µg/ml, for IF, IHC(P) and FCM
Answered by: Technical Support 5
Date published: 2017-01-19
  • y_2025, m_8, d_28, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_zh_CN, sid_13514, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 103ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Great productSuperb data for multiple applications including WB, IF and IHC staining
Date published: 2017-02-02
Rated 5 out of 5 by from Works Great!This antibody works great in western blot analysis of Pim-2 expression in CTLL-2 whole cell lysate.
Date published: 2017-01-11
Rated 5 out of 5 by from Published data for WB in mouse Bone marrowPublished data for WB in mouse Bone marrow. -SCBT Publication Review
Date published: 2015-04-26
Rated 5 out of 5 by from Publishable data for IHC and WB in humanPublishable data for IHC and WB in human H1299 cells. -SCBT Publication Review
Date published: 2015-04-13
Rated 4 out of 5 by from This antibody worked fineThis antibody worked fine.
Date published: 2015-03-26
Rated 5 out of 5 by from Very strong band in WB in human PimVery strong band in WB in human Pim-2 transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-03-03
Rated 5 out of 5 by from Great immunoperoxidase cytoplasmic stainingGreat immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded mouse lymphoma tissue. -SCBT QC
Date published: 2015-03-02
Rated 5 out of 5 by from Band of interest detected in WB in CTLLBand of interest detected in WB in CTLL-2 whole cell lysate. -SCBT QC
Date published: 2015-02-20
  • y_2025, m_8, d_28, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_11
  • loc_zh_CN, sid_13514, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 15ms
  • REVIEWS, PRODUCT
Pim-2 抗体 (1D12) is rated 4.9 out of 5 by 11.
  • y_2025, m_8, d_28, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_11
  • loc_zh_CN, sid_13514, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 105ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。