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Pim-1 Antibody (12H8) - Western Blotting - Image 375153
Pim-1 抗体 (12H8): sc-13513. HUV-EC-C (A), Raji (B) 和 M1 (C) 全细胞裂解液中 Pim-1 表达的 Western 印迹分析.
Pim-1 Antibody (12H8) - Western Blotting - Image 375153
Pim-1 抗体 (12H8): sc-13513. HUV-EC-C (A), Raji (B) 和 M1 (C) 全细胞裂解液中 Pim-1 表达的 Western 印迹分析.
Pim-1 Antibody (12H8) - Western Blotting - Image 375154
Pim-1 抗体 (12H8): sc-13513. MEG-01 全细胞裂解液中 Pim-1 表达的 Western 印迹分析.
Pim-1 Antibody (12H8) - Western Blotting - Image 1936
Pim-1 抗体 (12H8): sc-13513. K-562 全细胞裂解液中 Pim-1 表达的 Western 印迹分析.
Pim-1 抗体 (12H8): sc-13513. HUV-EC-C (A), Raji (B) 和 M1 (C) 全细胞裂解液中 Pim-1 表达的 Western 印迹分析.

Pim-1 抗体 (12H8): sc-13513

4.8(11)
寫評論提問

说明书
  • Pim-1抗体(12H8)是小鼠单克隆IgG1 κ, 在99篇文献中引用，规格为200 µg/ml
  • 针对全长Pim-1的mouse来源
  • 抗-Pim-1 抗体 (12H8) 推荐用于 WB 和 IP，检测mouse, rat 和human 来源的 Pim-1
  • Pim-1抗体（12H8）可与Alexa Fluor® 546或Alexa Fluor® 594结合，用于WB（RGB）、IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Pim-1 (12H8): sc-13513的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是Pim-1 Antibody (12H8) for WB applications. 的首选辅助检测试剂，这些试剂现与Pim-1 Antibody (12H8) 打包提供（请参阅下面的订购信息）。

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研究信息

関連項目

额外Pim抗体，包括Pim-1, Pim-2和Pim-3

Pim-1抗体（12H8）是一种IgG1 κ小鼠单克隆Pim-1抗体，可通过WB和IP检测来自小鼠、大鼠和人类的Pim-1。Pim-1抗体（12H8）既可以是非偶联的，也可以是多种偶联形式的抗Pim-1抗体。Pim-1是一种丝氨酸/苏氨酸激酶，可与c-Myc在淋巴细胞转化中协同作用。Pim-1的表达量在从早期到晚期G1期间增加，在G1/S边界和G2阶段保持较高水平。Pim-1在转录和转译水平上都受到调节，并且已被证明可被IL-2刺激诱导。Pim-1还在T细胞分化中发挥作用，并且已被证明可以刺激c-Myc介导的溶酶体前的凋亡。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Pim-1 抗体 (12H8) 参考文献:

  1. 了解Pim-1在癌症中的功能差异。  |  Ouhtit, A., et al. 2015. Front Biosci (Elite Ed). 7: 208-14. PMID: 25553374
  2. PIM-1：通过激活EGFR信号传导，在宫颈癌中发挥癌基因的作用。  |  Yang, H., et al. 2020. Int J Biol Markers. 35: 67-73. PMID: 32914663
  3. Pim-1激酶是衰老肺成纤维细胞炎症分泌组的正反馈调节因子  |  Gao, AY., et al. 2022. Am J Physiol Lung Cell Mol Physiol. 323: L685-L697. PMID: 36223640
  4. 靶向巨噬细胞 PIM-1，通过抑制线粒体 ROS/Cl- 外流信号通路，抑制 NLRP3 炎性体的激活，从而缓解骨关节炎。  |  Zhang, Z., et al. 2023. J Transl Med. 21: 452. PMID: 37422640
  5. 抑制DYRK1A通过抑制STAT3/Pim-1/NFAT通路，减轻肺动脉高压患者的血管重塑。  |  Lan, C., et al. 2024. Clin Exp Hypertens. 46: 2297642. PMID: 38147409
  6. Pim-1激酶在炎症信号通路中的作用。  |  Baek, HS., et al. 2024. Inflamm Res. 73: 1671-1685. PMID: 39079978
  7. 蛋白激酶 PIM-1 的普遍表达和细胞周期调控。  |  Liang, H., et al. 1996. Arch Biochem Biophys. 330: 259-65. PMID: 8660654
  8. IL-2 处理对人类胸腺胚泡细胞原癌基因 pim-1 和 c-myb 转录衰减的影响  |  Rohwer, F., et al. 1996. J Immunol. 157: 643-9. PMID: 8752912
  9. Pim-1激酶刺激c-Myc介导的死亡信号传导，从而激活类似于caspase-3（CPP32）的蛋白酶。  |  Mochizuki, T., et al. 1997. Oncogene. 15: 1471-80. PMID: 9333023
  10. Pim-1 蛋白的表达受其 5'- 非翻译区和翻译引发剂 elF-4E 的调控。  |  Hoover, DS., et al. 1997. Cell Growth Differ. 8: 1371-80. PMID: 9419425
  11. 有证据表明，Gfi-1 和 Pim-1 参与了与β-选择相关的前 T 细胞分化步骤。  |  Schmidt, T., et al. 1998. EMBO J. 17: 5349-59. PMID: 9736613
  12. Pim-1 激酶和 p100 合作增强 c-Myb 的活性。  |  Leverson, JD., et al. 1998. Mol Cell. 2: 417-25. PMID: 9809063

订购信息

产品名称产品编号规格价格数量收藏夹

Pim-1 抗体 (12H8)

sc-13513
200 µg/ml
$316.00

Pim-1 (12H8): m-IgG Fc BP-HRP 套装

sc-526549
200 µg Ab; 10 µg BP
$354.00

Pim-1 (12H8): m-IgG1 BP-HRP 套装

sc-531922
200 µg Ab; 20 µg BP
$354.00

Pim-1 抗体 (12H8) Alexa Fluor® 546

sc-13513 AF546
200 µg/ml
$357.00

Pim-1 抗体 (12H8) Alexa Fluor® 594

sc-13513 AF594
200 µg/ml
$357.00

Pim-1 抗体 (12H8) Alexa Fluor® 680

sc-13513 AF680
200 µg/ml
$357.00

Pim-1 抗体 (12H8) Alexa Fluor® 790

sc-13513 AF790
200 µg/ml
$357.00

Can this be used for flow cytometry? If so, is it surface or ICS?

Asked by: E R D
Thank you for your question. Pim-1 Antibody (12H8) is available conjugated to Alexa Fluor® 546 or Alexa Fluor® 594 for flow cytometry. The antibody was raised against full length of Pim-1 of mouse origin. Since no epitope mapping has so far been performed, there is no information on whether it binds a cytoplasmic or extracellular domain of the protein.
Answered by: Technical Support Europe
Date published: 2020-10-23

could It recognize PIM1-L， The longer varient?

Asked by: Wanya
Thank you for your question. This mouse monoclonal antibody, Pim-1 (12H8): sc-13513, is cross reactive with both isoforms of Pim-1. This is based on several published articles which reference this clone detecting both (e.g., PMID 19345670).
Answered by: Technical Support
Date published: 2017-08-01

What secondary reagent should I use for IHC if I am working with mice tissue samples?

Asked by: Trav11
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 4
Date published: 2017-01-06
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Rated 4 out of 5 by from Excellent antibodythis is a good antibody for western blot at 1:100, clear band
Date published: 2017-11-03
Rated 5 out of 5 by from Good choice for Western BlotWell published clone for use in Western Blot, strong bands seen in whole cell lysate.
Date published: 2017-01-16
Rated 4 out of 5 by from Weak but specific band in Bone Marrow Derived DCsI used this antibody on Flt3L bone marrow derived DCs. I used at 1:1000 dilution and detected with ChemdocMP and Thermo Fisher Pico (w. 1/10 Femto) substrate. Show weak but specific bands of 3 isoforms. Can extend exposure to 600 - 1200 seconds. Background is low.
Date published: 2016-11-28
Rated 5 out of 5 by from This antibody is really good to detect PimThis antibody is really good to detect Pim-1 isoforms 1, 2 and 3 by Western blotting. The lysis of mitochondria is required to reveal the 40 kDa isoform-3 of Pim-1. It also works for Immunofluorescence but little longer exposure time needed.
Date published: 2015-12-17
Rated 5 out of 5 by from Very good for western blot to detect long form PimVery good for western blot to detect long form Pim-1 and short form, 44 and 33 kDa. Signal is weak if used for IF.
Date published: 2015-10-20
Rated 5 out of 5 by from IP and WB published data with human and monkey CWRIP and WB published data with human and monkey CWR-R1, 293T, COS-1, LNCaP, PC3, and 22Rv1 - SCBT Publication Review
Date published: 2015-05-10
Rated 5 out of 5 by from Used in a publication for IHCP with humanUsed in a publication for IHCP with human prostatic denocarcwithoma - SCBT Publication Review
Date published: 2015-04-01
Rated 5 out of 5 by from Used with successUsed with success. (human WB)
Date published: 2015-01-14
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Pim-1抗体 (12H8) is rated 4.8 out of 5 by 11.
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