Date published: 2025-9-6
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PI 3-kinase p85α/β/p55γ Antibody (D-9) - Western Blotting - Image 352011
PI 3-kinase p85α/β/p55γ (D-9): sc-374534. SW480 (A), A-431 (B), KNRK (C) 和 COLO 205 (D) 全细胞裂解液中 PI 3-kinase p85α/β/p55γ 表达的 Western 印迹分析.
PI 3-kinase p85α/β/p55γ (D-9): sc-374534. SW480 (A), A-431 (B), KNRK (C) 和 COLO 205 (D) 全细胞裂解液中 PI 3-kinase p85α/β/p55γ 表达的 Western 印迹分析.

PI 3-kinase p85α/β/p55γ 抗体 (D-9): sc-374534

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说明书
  • PI 3-kinase p85 alpha / beta /p55 gamma 抗体 D-9 是小鼠单克隆 IgG1 κ，PI 3-kinase p85α/β/p55γ抗体, 在17篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列323-596位于human物种的PI 3-kinase p85β的内部区域内
  • PI 3-kinase p85 alpha / beta /p55 gamma 抗体 (D-9) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 PI 3-kinase p85α, PI 3-kinase p85β, and PI 3-kinase p55γ; 也和以下物种反应，包括： 和 canine and bovine
  • 抗PI 3-kinase p85 alpha / beta /p55 gamma抗体（D-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取PI 3-kinase p85α/β/p55γ (D-9): sc-374534的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 PI 3-kinase p85α/β/p55γ 抗体 (D-9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与PI 3-kinase p85α/β/p55γ 抗体 (D-9) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 PI 3-kinase 抗体包括 PI 3-kinase C2 α, PI 3-kinase C2 β, PI 3-kinase C2 γ, PI 3-kinase p100, PI 3-kinase p101, PI 3-kinase p110, PI 3-kinase p110 α, PI 3-kinase p110 β, PI 3-kinase p110 δ 和 PI 3-kinase p110 γ

PI 3-kinase p85α/β/p55γ抗体（D-9）是一种IgG1 κ小鼠单克隆PI 3-kinase p85 alpha/beta/p55 gamma抗体（也称为PI3K抗体、p85抗体、p55抗体或磷脂酰肌醇-3-激酶抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类的PI 3-kinase p85 alpha/beta/p55 gamma蛋白。PI 3-kinase p85α/β/p55γ抗体（D-9）既可作为非共轭的抗PI 3-kinase p85 alpha/beta/p55 gamma抗体形式，也可作为多种共轭的抗PI 3-kinase p85 alpha/beta/p55 gamma抗体形式，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。磷脂酰肌醇3-激酶（PI 3-kinase）磷酸化肌醇脂质肌醇环的3' OH位置，由p85和p110亚基组成。PI 3-kinase p85缺乏PI 3-激酶活性，并作为适配器将p110与活化的蛋白质酪氨酸激酶耦合。PI 3-kinase p85α和PI 3-kinase p85β都具有一个SH3和两个SH2结构域。PI 3-kinase p85α，也称为GRB1、磷脂酰肌醇3-激酶调节蛋白1或p85，是一种由724个氨基酸组成的蛋白质，以四种选择性剪接的同工型形式存在。它参与胰岛素代谢，PI 3-kinase p85α基因的缺陷与胰岛素抵抗有关。PI 3-kinase p85α在T细胞中被Cbl-b多泛素化，并具有多个磷酸化氨基酸残基，包括位于467位的磷酸化酪氨酸残基。PI 3-kinase p85γ包含1102个氨基酸，由映射到人类7号染色体的基因编码。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PI 3-kinase p85α/β/p55γ 抗体 (D-9) 参考文献:

  1. 严重胰岛素抵抗中人 p85 alpha 磷脂酰肌醇 3- 激酶的天然变体：一种胰岛素刺激脂质激酶活性受损的新型变体。  |  Baynes, KC., et al. 2000. Diabetologia. 43: 321-31. PMID: 10768093
  2. T细胞中Cbl-b介导的泛素化对PI3K的蛋白水解调节  |  Fang, D. and Liu, YC. 2001. Nat Immunol. 2: 870-5. PMID: 11526404
  3. 利用表达/克隆受体酪氨酸激酶靶蛋白的新方法克隆 PI3 激酶缔合作用 p85。  |  Skolnik, EY., et al. 1991. Cell. 65: 83-90. PMID: 1849461
  4. SHC和胰岛素受体底物1通过新型非SH2结构域与胰岛素受体的NPEY基序发生磷酸化酪氨酸依赖性相互作用。  |  Gustafson, TA., et al. 1995. Mol Cell Biol. 15: 2500-8. PMID: 7537849
  5. 胰岛素受体底物-1 和 SHC 中的非 SH2 结构域介导了它们与胰岛素样生长因子 I 受体的 NPEY 矩阵之间的磷酸化酪氨酸依赖性相互作用。  |  Craparo, A., et al. 1995. J Biol Chem. 270: 15639-43. PMID: 7541045
  6. 胰岛素受体直接激活磷脂酰肌醇 3'- 激酶。  |  Van Horn, DJ., et al. 1994. J Biol Chem. 269: 29-32. PMID: 8276809
  7. 胰岛素受体底物1与肌肉和大脑中磷脂酰肌醇3-激酶调节亚基的两种新型剪接变体结合。  |  Antonetti, DA., et al. 1996. Mol Cell Biol. 16: 2195-203. PMID: 8628286
  8. 磷酸肌酸 3- 激酶 p85 alpha 亚基断点簇区-同源结构域的晶体结构。  |  Musacchio, A., et al. 1996. Proc Natl Acad Sci U S A. 93: 14373-8. PMID: 8962058

订购信息

产品名称产品编号规格价格数量收藏夹

PI 3-kinase p85α/β/p55γ 抗体 (D-9)

sc-374534
200 µg/ml
$316.00

PI 3-kinase p85α/β/p55γ (D-9): m-IgG Fc BP-HRP 套装

sc-529764
200 µg Ab; 10 µg BP
$354.00

PI 3-kinase p85α/β/p55γ (D-9): m-IgGκ BP-HRP 套装

sc-522770
200 µg Ab, 40 µg BP
$354.00

PI 3-kinase p85α/β/p55γ (D-9): m-IgG1 BP-HRP 套装

sc-543676
200 µg Ab; 20 µg BP
$354.00

PI 3-kinase p85α/β/p55γ 抗体 (D-9) AC

sc-374534 AC
500 µg/ml, 25% agarose
$416.00

PI 3-kinase p85α/β/p55γ 抗体 (D-9) HRP

sc-374534 HRP
200 µg/ml
$316.00

PI 3-kinase p85α/β/p55γ 抗体 (D-9) FITC

sc-374534 FITC
200 µg/ml
$330.00

PI 3-kinase p85α/β/p55γ 抗体 (D-9) PE

sc-374534 PE
200 µg/ml
$343.00

PI 3-kinase p85α/β/p55γ 抗体 (D-9) Alexa Fluor® 488

sc-374534 AF488
200 µg/ml
$357.00

PI 3-kinase p85α/β/p55γ 抗体 (D-9) Alexa Fluor® 546

sc-374534 AF546
200 µg/ml
$357.00

PI 3-kinase p85α/β/p55γ 抗体 (D-9) Alexa Fluor® 594

sc-374534 AF594
200 µg/ml
$357.00

PI 3-kinase p85α/β/p55γ 抗体 (D-9) Alexa Fluor® 647

sc-374534 AF647
200 µg/ml
$357.00

PI 3-kinase p85α/β/p55γ 抗体 (D-9) Alexa Fluor® 680

sc-374534 AF680
200 µg/ml
$357.00

PI 3-kinase p85α/β/p55γ 抗体 (D-9) Alexa Fluor® 790

sc-374534 AF790
200 µg/ml
$357.00

Can PI 3-kinase p85α/β/p55γ (D-9): sc-374534 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: AbPolly
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, PI 3-kinase p85α/β/p55γ (D-9): sc-374534 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Good antibody for western blotThe antibody detects p85 alpha/ beta at 85-90 kDa and p85 gamma at 55 kDa in melanoma cells.
Date published: 2022-06-17
Rated 5 out of 5 by from Specific and robust monoWell-rounded antibody for Western blot, IHC and IF, dependable strong and clean. Great choice!
Date published: 2017-01-24
Rated 5 out of 5 by from ExcellentGot quick band, clean. Satisfied with the antibody. Thanks.
Date published: 2017-01-07
Rated 4 out of 5 by from work goodThis antibody works good, the band is clear and specific!
Date published: 2016-12-01
Rated 5 out of 5 by from Potent and very specific antibodyThis monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was strong detection of the ~84/53/50/84 kDa target proteins in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested.
Date published: 2016-11-10
Rated 5 out of 5 by from Good strong band in WB and IP in rat A7r5 cellsGood strong band in WB and IP in rat A7r5 cells. -SCBT Publication Review
Date published: 2015-04-16
Rated 5 out of 5 by from Produced exceptional immunoperoxidase cytoplasmicProduced exceptional immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human salivary gland tissue. -SCBT QC
Date published: 2015-03-12
Rated 5 out of 5 by from Good WB antibody in transfected mouse 293TGood WB antibody in transfected mouse 293T whole cell lysates. -SCBT QC
Date published: 2015-01-15
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PI 3-kinase p85α/β/p55γ 抗体 (D-9) is rated 4.9 out of 5 by 9.
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