Date published: 2025-11-4
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western blotting image from recommended PGC1a (D-5) monoclonal antibody replacement. Image 390096
这种多克隆抗体已经停产。请参阅我们推荐的PGC1a (D-5)单克隆抗体(显示样本数据)。
western blotting image from recommended PGC1a (D-5) monoclonal antibody replacement. Image 390096
这种多克隆抗体已经停产。请参阅我们推荐的PGC1a (D-5)单克隆抗体(显示样本数据)。
这种多克隆抗体已经停产。请参阅我们推荐的PGC1a (D-5)单克隆抗体(显示样本数据)。

PGC-1α 抗体 (H-300): sc-13067

4.0(16)
寫評論提問

  • PGC-1 alpha 抗体 H-300 是兔多克隆 IgG，提供 200 µg/ml
  • 抗原表位对应的氨基酸序列1-300位于human物种的PGC-1的N-terminus
  • 下架的多克隆抗体

関連項目

此外 PGC 抗体包括 PGC-1 α 和 PGC-1 β

订购信息

PGC1a (D-5): sc-518025 [用于替代PGC-1α (H-300)的推荐单抗]

产品名称产品编号规格价格数量收藏夹

PGC1a 抗体 (D-5)

sc-518025
200 µg/ml
$316.00

PGC1a (D-5): m-IgG Fc BP-HRP 套装

sc-531597
200 µg Ab; 10 µg BP
$354.00

PGC1a (D-5): m-IgGκ BP-HRP 套装

sc-525331
200 µg Ab, 40 µg BP
$354.00

PGC1a (D-5): m-IgG2a BP-HRP 套装

sc-547825
200 µg Ab; 10 µg BP
$354.00

PGC1a 抗体 (D-5) AC

sc-518025 AC
500 µg/ml, 25% agarose
$416.00

PGC1a 抗体 (D-5) HRP

sc-518025 HRP
200 µg/ml
$316.00

PGC1a 抗体 (D-5) FITC

sc-518025 FITC
200 µg/ml
$330.00

PGC1a 抗体 (D-5) PE

sc-518025 PE
200 µg/ml
$343.00

PGC1a 抗体 (D-5) Alexa Fluor® 488

sc-518025 AF488
200 µg/ml
$357.00

PGC1a 抗体 (D-5) Alexa Fluor® 546

sc-518025 AF546
200 µg/ml
$357.00

PGC1a 抗体 (D-5) Alexa Fluor® 594

sc-518025 AF594
200 µg/ml
$357.00

PGC1a 抗体 (D-5) Alexa Fluor® 647

sc-518025 AF647
200 µg/ml
$357.00

PGC1a 抗体 (D-5) Alexa Fluor® 680

sc-518025 AF680
200 µg/ml
$357.00

PGC1a 抗体 (D-5) Alexa Fluor® 790

sc-518025 AF790
200 µg/ml
$357.00

If the product is specified for mice, can it work on rat tissue¿

Asked by: XimenaYri
Thank you for your question. PGC-1α(H-300) was recommended for detection of PGC-1α of mouse, rat and human origin. This antibody has been discontinued. We recommend you use one of our monoclonal alternatives, such as PGC-1α Anticuerpo (D-5): sc-518025, which is recommended for detection of PGC-1α of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA.
Answered by: Tech Support Europe
Date published: 2021-11-09

what is the working concentration to be used

Asked by: Thara
Thank you for your question. Please refer to our protocols for the recommended concentrations for our antibodies. If you have any further questions, please contact Technical Service. https://www.scbt.com/resources/protocols
Answered by: Technical Service 3
Date published: 2020-11-25

I am getting a band between 37 and 50 kDa with this antibody. Has someone else reported this?

Asked by: Kranti
Thank you for your question. The expected molecular weight of PGC-1α is 90 kDa, but do not have information regarding a smaller size. We recommend using our monoclonal alternative PGC-1α Antibody (D-5): sc-518025. Please contact our technical service if you require further information.
Answered by: Technical Support
Date published: 2020-11-02

What is the expected molecular weight for bands?

Asked by: JJJJ55
Thank you for your question. The expected molecular weight of PGC-1α is 90 kDa. We recommend using our monoclonal alternative PGC-1α Antibody (D-5): sc-518025. Please contact our technical service if you require further information.
Answered by: SCBT Heidelberg Support
Date published: 2019-03-07

I performed an immunoprecipitation using this antibody (PGC-1 alpha Antibody - H-300) and I had a problem since the heavy chain (HC, 55 kDa) of the IP antibody were also recognized.

Asked by: johnny86
Thank you for your question. This is rabbit polyclonal antibody, PGC-1α (H-300): sc-13067, is expected to detect the PGC-1α protein at approximately 91 kDa. The heavy chain of IgG is approximately 55 kDa. Therefore, observing the heavy chain IgG band from your immunoprecipitating antibody should not interfere with detection of your target protein. However, if you wish to not observe the heavy chain, then we would recommend using a different antibody for your Western blot. For example, a mouse monoclonal against PGC-1α. Then you could use an anti-mouse IgG secondary antibody, that would not recognize the heavy chain of the rabbit IgG primary used for immunoprecipitation.
Answered by: Technical Support
Date published: 2018-08-17

I used this antibody on cells and get 2 bands very close together which one is pgc1 alpha upper or lower

Asked by: Joey88
Thank you for your question. We are sorry to hear that this antibody did not perform as expected. It is very difficult to say which band is which without having more information. Please reach out to our Technical Service Department by phone, e-mail or live chat. We are happy to help you troubleshoot the antibody and make sure you get the results you are looking for!
Answered by: Tech Service
Date published: 2017-06-07
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Rated 2 out of 5 by from OKI bought this few months ago, it did not perform really well in western blot
Date published: 2018-09-28
Rated 5 out of 5 by from Highly specific antibodyWe bought this antibody for use in western, IF and IHC and we are highly satisfied by its performance. WB- 1:1000, IF- 1:250, IHC- 1:200
Date published: 2017-03-09
Rated 5 out of 5 by from Worked well in WBI used this antibody in WB (dilution 1:500), the band is wonderful.
Date published: 2016-11-01
Rated 5 out of 5 by from worked wellI used this antibody in WB (dilution 1:500), the band is wonderful.
Date published: 2016-10-31
Rated 1 out of 5 by from Multiple unspecific bands across antibodyMultiple unspecific bands across antibody dilutions (1:100-10:1000) and independent of protein loading (20-80ug). No band at expected size. Mouse liver tissues were used.
Date published: 2016-01-12
Rated 4 out of 5 by from This has worked well in WBThis has worked well in WB.
Date published: 2015-11-04
Rated 5 out of 5 by from This has worked well in WBThis has worked well in WB
Date published: 2015-10-27
Rated 4 out of 5 by from Used in IF and got good staining at 1:100 dilutionUsed in IF and got good staining at 1:100 dilution.
Date published: 2015-09-24
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PGC-1α 抗体 (H-300) is rated 4.0 out of 5 by 16.
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