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Pcf11 Antibody (C-9) - Western Blotting - Image 317951
Pcf11 抗体 (C-9): sc-514158. CCRF-CEM (A), HeLa (B), Jurkat (C) 和 HEK293T (D) 全细胞裂解液及 HeLa 核提取物 (E) 中 Pcf11 表达的 Western 印迹分析.
Pcf11 Antibody (C-9) - Western Blotting - Image 317951
Pcf11 抗体 (C-9): sc-514158. CCRF-CEM (A), HeLa (B), Jurkat (C) 和 HEK293T (D) 全细胞裂解液及 HeLa 核提取物 (E) 中 Pcf11 表达的 Western 印迹分析.
Pcf11 抗体 (C-9): sc-514158. CCRF-CEM (A), HeLa (B), Jurkat (C) 和 HEK293T (D) 全细胞裂解液及 HeLa 核提取物 (E) 中 Pcf11 表达的 Western 印迹分析.

Pcf11 抗体 (C-9): sc-514158

5.0(1)
寫評論提問

说明书
  • Pcf11抗体(C-9)是小鼠单克隆IgG3 κ, 在1篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的Pcf11的内部区域内的氨基酸737-767之间
  • 推荐用于 mouse, rat 和 human 来源的Pcf11 WB, IP, IF 和 ELISA检测
  • 关于如何获取Pcf11 (C-9): sc-514158的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成Pcf11 抗体 (C-9)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

Pcf11抗体（C-9）是一种IgG3 κ小鼠单克隆Pcf11抗体，可通过WB, IP, IF 和 ELISA检测小鼠、大鼠和人类来源的Pcf11。Pcf11抗体（C-9）以非偶联的抗Pcf11抗体形式提供。在酿酒酵母中，切割/多聚腺苷酸化因子Pcf11是将多聚腺苷酸化机械招募到延伸的RNA聚合酶II（RNAPII）所需的关键调节因子，对于正确的转录终止是必需的。Pcf11（PCF11，切割和多聚腺苷酸化因子亚基，同源物（酿酒酵母））是一种含有1555个氨基酸的核蛋白，是pre-mRNA切割复合体II的组成部分。有研究表明，Pcf11能够促进Pol II延伸复合物从DNA中解离。Pcf11包含一个CTD相互作用结构域，该结构域以磷酸依赖性方式与RNA聚合酶II CTD内的七肽重复序列结合。编码Pcf11的基因位于人类第11号染色体上，该染色体包含1400多个基因，约占人类基因组的4%。Jervell和Lange-Nielsen综合征、Jacobsen综合征、Niemann-Pick病、遗传性血管性水肿和Smith-Lemli-Opitz综合征与映射到11号染色体的基因缺陷有关。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Pcf11 抗体 (C-9) 参考文献:

  1. 人类前核糖核酸裂解因子 II（m）含有酵母蛋白的同源物，并与其他两种裂解因子连接。  |  de Vries, H., et al. 2000. EMBO J. 19: 5895-904. PMID: 11060040
  2. 酵母前 mRNA 3' 端处理因子与 RNA 聚合酶 II 的功能性相互作用。  |  Licatalosi, DD., et al. 2002. Mol Cell. 9: 1101-11. PMID: 12049745
  3. 终止, 3'加工和 mRNA 导出的耦合。  |  Hammell, CM., et al. 2002. Mol Cell Biol. 22: 6441-57. PMID: 12192043
  4. 3'-RNA 处理因子识别 RNA 聚合酶 II 羧基末端结构域。  |  Meinhart, A. and Cramer, P. 2004. Nature. 430: 223-6. PMID: 15241417
  5. Pcf11 CID 与 RNA 聚合酶 II CTD 之间相互作用的主要特征。  |  Noble, CG., et al. 2005. Nat Struct Mol Biol. 12: 144-51. PMID: 15665873
  6. RNA 聚合酶 II CTD 磷酸肽与 RNA 竞争与 Pcf11 的相互作用。  |  Hollingworth, D., et al. 2006. RNA. 12: 555-60. PMID: 16497660
  7. 与核苷酸结合的 Clp1-Pcf11 多腺苷酸化因子的结构。  |  Noble, CG., et al. 2007. Nucleic Acids Res. 35: 87-99. PMID: 17151076
  8. 转录终止因子Pcf11限制了Pol II在HIV前病毒上抑制基因表达的过程。  |  Zhang, Z., et al. 2007. Genes Dev. 21: 1609-14. PMID: 17606639
  9. 人类 Pcf11 可增强 RNA 聚合酶 II 缔合作用新生 RNA 的降解和转录终止。  |  West, S. and Proudfoot, NJ. 2008. Nucleic Acids Res. 36: 905-14. PMID: 18086705

订购信息

产品名称产品编号规格价格数量收藏夹

Pcf11 抗体 (C-9)

sc-514158
200 µg/ml
$316.00

Pcf11 (C-9) 中和勝肽

sc-514158 P
100 µg/0.5 ml
$68.00

Hello, For the PCF11 antibody sc-514158, what is the lower band in the image here (a little above 120kDa). This is the only band I see when I use protein extracts from mouse cell line. Thanks!

Asked by: SMM24
Thank you for your question. For assistance please contact technical service by emailing scbt@scbt.com or call (800)-457-3801.
Answered by: BlakeJ
Date published: 2024-03-28

Hello! Would it be possible to know the amount (ng) of protein loaded on the illustrative Western blot and the antibody dilution used? Also would it be possible to get a free sample of the antibody? Thank you very much.

Asked by: JeromeR
Thank you for your question. This western blot was created with our recommended protocol, which you can find by following this link: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting You can find the datasheet with the ideal dilutions for this antibody here: https://datasheets.scbt.com/sc-514158.pdf 40–60 µg whole cell lysate and 10–20 µg nuclear extract were used in each lane of these product images. If your samples are transfected lysates, use 5-10 µg protein or use 10–20 ng for purified protein. To arrange for a free sample of this antibody please get in touch with our technical service.
Answered by: Technical Support Europe
Date published: 2019-04-30

What application is the blocking peptide sc-514158 P appropriate for?

Asked by: jerojero
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Great Western blot data of Pcf11 expressionGreat Western blot data of Pcf11 expression in CCRF-CEM, HeLa, Jurkat and HEK293T whole cell lysates and HeLa nuclear extract . -SCBT QC
Date published: 2014-10-31
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Pcf11 抗体 (C-9) is rated 5.0 out of 5 by 1.
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