Date published: 2025-9-8

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PACSIN2 抗体 (F-12): sc-390136

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说明书
  • PACSIN2 抗体 F-12 是小鼠单克隆 IgG1 (kappa轻链),PACSIN2抗体,规格为200 µg/ml
  • 免疫原氨基酸序列171-316位于human物种的PACSIN2的内部区域内
  • PACSIN2 抗体 (F-12) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 PACSIN2
  • 抗PACSIN2抗体(F-12)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取PACSIN2 (F-12): sc-390136免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 PACSIN2 抗体 (F-12) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与PACSIN2 抗体 (F-12) 组合成套装提供。 (请参阅下面的订购信息)。

    快捷链接

    関連項目

    PACSIN2抗体(F-12)是一种IgG1 κ小鼠单克隆PACSIN2抗体,可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的PACSIN2蛋白。PACSIN2抗体(F-12)既可以是非偶联的抗PACSIN2抗体形式,也可以是多种偶联形式的抗PACSIN2抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。PACSINs是细胞质衔接蛋白家族的成员,具有保守的C端蛋白质结合SH3结构域和CDC15-NT结构域。与PACSIN1相关的蛋白质包括syndapin 1(PACSIN1的大鼠同源物)、FAP52、EM13和PSTPIP,这些蛋白质似乎都参与与细胞骨架组织相关的信号通路。PACSIN1的表达仅限于终末分化的神经组织,而PACSIN2则广泛表达。PACSIN2呈囊泡状分布,可能参与调节内吞过程。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    PACSIN2 抗体 (F-12) 参考文献:

    1. PACSIN 2,细胞质适配蛋白 PACSIN 家族的新成员。  |  Ritter, B., et al. 1999. FEBS Lett. 454: 356-62. PMID: 10431838
    2. PACSIN2与非结构蛋白5A相互作用并调节丙型肝炎病毒组装。  |  Nguyen, LP., et al. 2020. J Virol. 94: PMID: 31801866
    3. 交界的 PACSIN2/EHD4/MICAL-L1 复合物可协调 VE-cadherin迁移,促进内皮迁移和血管生成。  |  Malinova, TS., et al. 2021. Nat Commun. 12: 2610. PMID: 33972531
    4. 洞穴内 PACSIN2 和 EHD2 的超分辨率分析。  |  Nishimura, T. and Suetsugu, S. 2022. PLoS One. 17: e0271003. PMID: 35834519
    5. PACSIN2 作为自噬和巯嘌呤细胞毒性的调节剂:淋巴细胞和肠细胞的机制。  |  Zudeh, G., et al. 2023. Life Sci Alliance. 6: PMID: 36596605
    6. PACSIN2调节血小板整合素β1的止血功能。  |  Biswas, R., et al. 2023. J Thromb Haemost. 21: 3619-3632. PMID: 37678551
    7. 分子克隆出一种对多形性棘球蚴病有免疫反应的棘球蚴微滴虫抗原。  |  Frosch, PM., et al. 1993. Mol Biochem Parasitol. 58: 301-10. PMID: 8479454
    8. FAP52,一种新型, 含 SH3 结构域的焦点粘附蛋白。  |  Meriläinen, J., et al. 1997. J Biol Chem. 272: 23278-84. PMID: 9287337
    9. 酪氨酸磷酸化调节 SH3 介导的 Wiskott-Aldrich 综合征蛋白与细胞骨架缔合蛋白 PSTPIP 的结合作用。  |  Wu, Y., et al. 1998. J Biol Chem. 273: 5765-70. PMID: 9488710
    10. PACSIN 是一种脑蛋白,在分化成神经细胞时会上调。  |  Plomann, M., et al. 1998. Eur J Biochem. 256: 201-11. PMID: 9746365
    11. Syndapin I,一种能与神经 Wiskott-Aldrich 综合征蛋白缔合作用的突触 dynamin 结合蛋白。  |  Qualmann, B., et al. 1999. Mol Biol Cell. 10: 501-13. PMID: 9950691

    订购信息

    产品名称产品编号规格价格数量收藏夹

    PACSIN2 抗体 (F-12)

    sc-390136
    200 µg/ml
    $316.00

    PACSIN2 (F-12): m-IgG Fc BP-HRP 套装

    sc-530075
    200 µg Ab; 10 µg BP
    $354.00

    PACSIN2 (F-12): m-IgGκ BP-HRP 套装

    sc-523420
    200 µg Ab, 40 µg BP
    $354.00

    PACSIN2 (F-12): m-IgG1 BP-HRP 套装

    sc-543857
    200 µg Ab; 20 µg BP
    $354.00

    PACSIN2 抗体 (F-12) AC

    sc-390136 AC
    500 µg/ml, 25% agarose
    $416.00

    PACSIN2 抗体 (F-12) HRP

    sc-390136 HRP
    200 µg/ml
    $316.00

    PACSIN2 抗体 (F-12) FITC

    sc-390136 FITC
    200 µg/ml
    $330.00

    PACSIN2 抗体 (F-12) PE

    sc-390136 PE
    200 µg/ml
    $343.00

    PACSIN2 抗体 (F-12) Alexa Fluor® 488

    sc-390136 AF488
    200 µg/ml
    $357.00

    PACSIN2 抗体 (F-12) Alexa Fluor® 546

    sc-390136 AF546
    200 µg/ml
    $357.00

    PACSIN2 抗体 (F-12) Alexa Fluor® 594

    sc-390136 AF594
    200 µg/ml
    $357.00

    PACSIN2 抗体 (F-12) Alexa Fluor® 647

    sc-390136 AF647
    200 µg/ml
    $357.00

    PACSIN2 抗体 (F-12) Alexa Fluor® 680

    sc-390136 AF680
    200 µg/ml
    $357.00

    PACSIN2 抗体 (F-12) Alexa Fluor® 790

    sc-390136 AF790
    200 µg/ml
    $357.00

    I am using PACSIN2 (F-12): sc-390136 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

    Asked by: Trav11
    We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
    Answered by: Technical Support
    Date published: 2017-02-25
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    Rated 5 out of 5 by from Great for Fluorescent ImmunoHistologyTried the Sample test for Fluorescence ImmunoHistology -Brain perfused with 4% PFA, transcardially -tried embedded AND non-embedded free-floting section (works good on both-40um) -"Antigene retrieval" at 80° in 10mM NatriumCitrate -PBS buffer -Concentration 1:100 and 1:200
    Date published: 2018-01-04
    Rated 3 out of 5 by from PACSIN2 AntibodyI used a sample product to detect PACSIN2 in HeLa cells for immunofluorescence (1:100). I did work well, but there was a bit of background.
    Date published: 2017-10-12
    Rated 5 out of 5 by from Highly specificWe asked samples from Santa two weeks ago and performed IF to test the specificity. It had no signal from nuclei and showed dots like pattern under confoal microscopy which is consistent with reported data and our living-cell results.
    Date published: 2017-09-25
    Rated 5 out of 5 by from Produced positive Western blot data of PACSIN2Produced positive Western blot data of PACSIN2 expression in SHP-77, F9, SK-N-MC and IMR-32 whole cell lysates. -SCBT QC
    Date published: 2013-07-17
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    PACSIN2 抗体 (F-12) is rated 4.5 out of 5 by 4.
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