Date published: 2025-9-8
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p67-phox Antibody (D-6) - Western Blotting - Image 363437
p67-phox 抗体 (D-6): sc-374510. RAW 264. 7 全细胞裂解液中 p67-phox 表达的 Western 印迹分析.
p67-phox 抗体 (D-6): sc-374510. RAW 264. 7 全细胞裂解液中 p67-phox 表达的 Western 印迹分析.

p67-phox 抗体 (D-6): sc-374510

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说明书
  • p67-phox 抗体 D-6 是小鼠单克隆 IgG1 κ，p67-phox抗体, 在32篇文献中引用，规格为200 µg/ml
  • 免疫human物种的p67-phox的氨基酸1-300
  • p67-phox 抗体 (D-6) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 p67-phox
  • 抗p67-phox抗体（D-6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取p67-phox (D-6): sc-374510的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 p67-phox 抗体 (D-6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与p67-phox 抗体 (D-6) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
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说明书与实验方案
研究信息

関連項目

p67-phox抗体（D-6）是一种IgG1 κ小鼠单克隆p67-phox抗体（也称为NCF2抗体、中性粒细胞胞质因子2抗体或P67PHOX抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人源的p67-phox蛋白。p67-phox抗体（D-6）既可以是非偶联的抗p67-phox抗体形式，也可以是多种偶联形式的抗p67-phox抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。遗传性疾病慢性肉芽肿病（CGF）已与p47phox和p67phox的突变有关。胞质蛋白p47phox和p67phox（分别也称为中性粒细胞胞质因子1（NCF1）和NCF2）是激活中性粒细胞和其他吞噬细胞中产生超氧化物的NADPH氧化酶所必需的。在NADPH氧化酶激活过程中，p47phox和p67phox迁移到质膜上，与细胞色素b558和小G蛋白Rac结合，形成有功能的酶复合物。p47phox和p67phox都包含两个Src同源3（SH3）结构域。已显示p67phox的C端SH3结构域与p47phox的富含脯氨酸结构域相互作用，表明p47phox可能促进p67phox向质膜的转运。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

p67-phox 抗体 (D-6) 参考文献:

  1. 中性粒细胞烟酰胺腺嘌呤二核苷酸磷酸氧化酶的组装。p47-phox和p67-phox的转移需要p47-phox和细胞色素b558之间的相互作用。  |  Heyworth, PG., et al. 1991. J Clin Invest. 87: 352-6. PMID: 1985107
  2. p67(phox) 终止子磷脂酶 A(2) 发出的激活 NADPH 氧化酶（NOX2）的信号。  |  Krishnaiah, SY., et al. 2013. FASEB J. 27: 2066-73. PMID: 23401562
  3. 重组 47 千道尔顿细胞因子可恢复慢性肉芽肿病中的 NADPH 氧化酶。  |  Lomax, KJ., et al. 1989. Science. 245: 409-12. PMID: 2547247
  4. 运动训练可减轻糖尿病大鼠心脏中 p47(phox) 和 p67(phox) 的上调。  |  Sharma, NM., et al. 2016. Oxid Med Cell Longev. 2016: 5868913. PMID: 26989452
  5. Nox2 和 p67phox 中 369 Cys-Gly-Cys371 三元组的半胱氨酸之间的二硫键稳定了 p67phox 与 Nox2 的结合。  |  Fradin, T., et al. 2018. J Leukoc Biol. 104: 1023-1039. PMID: 30011082
  6. p67phox 衍生的自组装肽通过一种自身抑制机制阻止了 Nox2 NADPH 氧化酶的激活。  |  Bechor, E., et al. 2021. J Leukoc Biol. 109: 657-673. PMID: 32640488
  7. 缺血/再灌注时 p67phox 的上调可通过 STAT3 增加 ZIP2 的表达，从而起到保护心脏的作用。  |  Zhao, L., et al. 2022. Free Radic Res. 56: 115-126. PMID: 35296207
  8. 流式细胞仪分析 p67phox 缺失型慢性肉芽肿病中活性氧生成和残余 p67phox 表达的临床和分子意义。  |  Miyazawa, H., et al. 2024. Scand J Immunol. 100: e13372. PMID: 38654426
  9. p67phox 基因的特征：用于慢性肉芽肿病产前诊断的基因组组织和限制性片段长度多态性分析。  |  Kenney, RT., et al. 1993. Blood. 82: 3739-44. PMID: 7903171
  10. 一个 SH3 结构域和富含脯氨酸的序列介导了吞噬细胞 NADPH 氧化酶复合物两个成分之间的相互作用。  |  Finan, P., et al. 1994. J Biol Chem. 269: 13752-5. PMID: 8188650
  11. p47-phox假基因携带着导致p47-phox缺陷型慢性肉芽肿病的最常见突变。  |  Görlach, A., et al. 1997. J Clin Invest. 100: 1907-18. PMID: 9329953
  12. 通过其内在荧光研究白细胞 NADPH 氧化酶亚基 p47(phox) 在活化过程中的构象变化。  |  Park, HS. and Park, JW. 1998. Biochim Biophys Acta. 1387: 406-14. PMID: 9748657

订购信息

产品名称产品编号规格价格数量收藏夹

p67-phox 抗体 (D-6)

sc-374510
200 µg/ml
$316.00

p67-phox (D-6): m-IgG Fc BP-HRP 套装

sc-529760
200 µg Ab; 10 µg BP
$354.00

p67-phox (D-6): m-IgGκ BP-HRP 套装

sc-522762
200 µg Ab, 40 µg BP
$354.00

p67-phox (D-6): m-IgG1 BP-HRP 套装

sc-543675
200 µg Ab; 20 µg BP
$354.00

p67-phox 抗体 (D-6) AC

sc-374510 AC
500 µg/ml, 25% agarose
$416.00

p67-phox 抗体 (D-6) HRP

sc-374510 HRP
200 µg/ml
$316.00

p67-phox 抗体 (D-6) FITC

sc-374510 FITC
200 µg/ml
$330.00

p67-phox 抗体 (D-6) PE

sc-374510 PE
200 µg/ml
$343.00

p67-phox 抗体 (D-6) Alexa Fluor® 488

sc-374510 AF488
200 µg/ml
$357.00

p67-phox 抗体 (D-6) Alexa Fluor® 546

sc-374510 AF546
200 µg/ml
$357.00

p67-phox 抗体 (D-6) Alexa Fluor® 594

sc-374510 AF594
200 µg/ml
$357.00

p67-phox 抗体 (D-6) Alexa Fluor® 647

sc-374510 AF647
200 µg/ml
$357.00

p67-phox 抗体 (D-6) Alexa Fluor® 680

sc-374510 AF680
200 µg/ml
$357.00

p67-phox 抗体 (D-6) Alexa Fluor® 790

sc-374510 AF790
200 µg/ml
$357.00

Whether the HRP Conjugated p67 antibody will recognize denatured sample??

Asked by: Anonymous
Thank you for your question. Anti-p67-phox Antibody (D-6) is recommended for detection of p67-phox of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA and as such detects both denatured and native p67 protein.
Answered by: Tech Support Europe
Date published: 2021-03-22

In addition to P67 I get a quite strong band at approximately 47kD in my rat heart samples. Any idea what it can be

Asked by: thomasa
Thank you for your message. Please contact our Technical Service. We are happy to help you troubleshoot the antibody.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-21

Can p67-phox (D-6): sc-374510 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: jerojero
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, p67-phox (D-6): sc-374510 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from sc-374510worked great for western blot and worked great for IP. highly recommended for experiment
Date published: 2019-12-04
Rated 5 out of 5 by from Excellent antibody used for WB on porcine sampleAntibody was used on porcine heart lysate, membrane fraction at dilution of 1:150. Consistently showed bands at around 67 kDa and more.
Date published: 2017-12-07
Rated 5 out of 5 by from good resultDetected p67phox in the diabetic kidney rats, and we cited this antibody in our published literature.We recommend 1:800 as the optimum dilution .
Date published: 2017-09-16
Rated 1 out of 5 by from Mauvais anticorpsIl ne fonctionne pas dans les conditions recommandées par Santa Cruz. Nous avons testé des extraits cellulaires totaux obtenus à partir de lignées cellulaires de myélome multiple, séparés par SDS-PAGE.
Date published: 2017-08-21
Rated 5 out of 5 by from Very good!!We tested p67phox by western blotting analysis. 60 micrograms of mouse brain membrane lysates were resolved on 10% SDS-PAGE, and incubated over night with monoclonal p67phox antibody (1:1000). Secondary antibody utilized was anti-mouse sc-2005 antibody (1:5000). p67phox signal was detected by chemidoc (Biorad) and the signal was reached since short exposition. Based on our experience p67phox is a good antibody
Date published: 2017-07-20
Rated 5 out of 5 by from Class p67 - phox transfection 293 t cell lysatentibody positive immunoblot non - transfected p67 - phox expression data and human p67 - phox transfection 293 t cell lysate
Date published: 2017-02-09
Rated 5 out of 5 by from Very strong antibody!Excellent antibody for Western blot, strong specific bands in mouse and human samples. Great choice!
Date published: 2017-02-01
Rated 5 out of 5 by from Produced positive Western blot data of p67Produced positive Western blot data of p67-phox expression in non-transfected and human p67-phox transfected 293T whole cell lysates. -SCBT QC
Date published: 2014-07-17
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p67-phox 抗体 (D-6) is rated 4.4 out of 5 by 8.
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