P2Y12 Double Nickase Plasmid (m): sc-427818-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- P2Y12 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- P2Y12 Double Nickase Plasmid (m) and P2Y12 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting P2ry12. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
P2Y12 Double Nickase Plasmid (m) | sc-427818-NIC | 20 µg | $410.00 | |||
P2Y12 Double Nickase Plasmid (m2) | sc-427818-NIC-2 | 20 µg | $410.00 |
Mouse P2ry12 encodes the purinergic receptor P2Y12, a Gi-coupled GPCR that senses extracellular ADP to suppress cAMP signaling and coordinate downstream PI3K–Akt and MAPK responses. In the central nervous system, P2Y12 is highly enriched in microglia where it regulates process motility, chemotaxis, and surveillance behaviors in response to purinergic cues released by stressed or damaged cells. P2Y12-dependent signaling influences neuroimmune communication, synaptic remodeling, and inflammatory tone, making P2ry12 a widely used molecular marker and functional node in microglial biology. Dysregulated purinergic signaling through P2Y12 has been associated with altered microglial activation states in models of neuroinflammation and neurodegeneration.
P2Y12 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the P2ry12 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within P2ry12. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt P2ry12 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of P2ry12-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.