Date published: 2025-9-11
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Phosphotyrosine Antibody (PY20) - Western Blotting - Image 5571
p-Tyr 抗体 (PY20) HRP: sc-508 HRP. 用 1. 0 µg/ml (A), 0. 5 µg/ml (B) 和 0. 2 µg/ml (C) 的 p-Tyr 抗体 (PY20) HRP 对从 A-431 细胞免疫沉淀的 EGF 受体进行 Western 印迹分析.
p-Tyr 抗体 (PY20) HRP: sc-508 HRP. 用 1. 0 µg/ml (A), 0. 5 µg/ml (B) 和 0. 2 µg/ml (C) 的 p-Tyr 抗体 (PY20) HRP 对从 A-431 细胞免疫沉淀的 EGF 受体进行 Western 印迹分析.

Phosphotyrosine 抗体 (PY20): sc-508

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说明书
  • Phosphotyrosine抗体(PY20)是小鼠单克隆IgG2b κ, 在1,325篇文献中引用，规格为200 µg/ml
  • 针对p-Tyr
  • 建议使用Phosphotyrosine抗体（PY20）通过WB, IP, IF, IHC(P)和FCM检测broad species
  • 抗Phosphotyrosine抗体（PY20）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Phosphotyrosine (PY20): sc-508的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 Phosphotyrosine 抗体 (PY20) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Phosphotyrosine 抗体 (PY20) 组合成套装提供。 (请参阅下面的订购信息)。

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研究信息

関連項目

此外 p-Tyr 抗体包括 p-Ser 和 p-Thr

p-Tyr抗体（PY20）是一种小鼠单克隆IgG2b kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和流式细胞术（FCM）检测多种物种中的磷酸化酪氨酸。抗磷酸化酪氨酸抗体（PY20）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合型。磷酸化酪氨酸在细胞信号传导中起着至关重要的作用，特别是在调节蛋白酪氨酸激酶方面，后者介导信号转导通路。这些激酶分为两大类：受体酪氨酸激酶和非受体酪氨酸激酶，Src激酶是后者的一个重要例子。Src激酶通常定位于内质膜，与缺乏细胞内催化结构域的表面受体一起作为信号转导器。受体酪氨酸激酶包括14个家族的50多个成员，配体诱导的酪氨酸磷酸化会触发受体二聚化，并使细胞质结构域内的特定磷酸化酪氨酸残基自磷酸化。这些磷酸化残基充当细胞质信号分子的结合位点，影响各种细胞过程。磷酸化酪氨酸单克隆抗体（PY20）是鉴定和纯化含磷酸化酪氨酸蛋白的宝贵工具，有助于我们加深对细胞信号传导和功能的理解。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Phosphotyrosine 抗体 (PY20)

sc-508
200 µg/ml
$316.00

Phosphotyrosine (PY20): m-IgG Fc BP-HRP 套装

sc-528116
200 µg Ab; 10 µg BP
$354.00

Phosphotyrosine (PY20): m-IgGκ BP-HRP 套装

sc-520417
200 µg Ab, 40 µg BP
$354.00

Phosphotyrosine (PY20): m-IgG2b BP-HRP 套装

sc-549995
200 µg Ab; 10 µg BP
$354.00

Phosphotyrosine 抗体 (PY20) AC

sc-508 AC
500 µg/ml, 25% agarose
$416.00

Phosphotyrosine 抗体 (PY20) HRP

sc-508 HRP
200 µg/ml
$316.00

Phosphotyrosine 抗体 (PY20) FITC

sc-508 FITC
200 µg/ml
$330.00

Phosphotyrosine 抗体 (PY20) PE

sc-508 PE
200 µg/ml
$343.00

Phosphotyrosine 抗体 (PY20) Alexa Fluor® 488

sc-508 AF488
200 µg/ml
$357.00

Phosphotyrosine 抗体 (PY20) Alexa Fluor® 546

sc-508 AF546
200 µg/ml
$357.00

Phosphotyrosine 抗体 (PY20) Alexa Fluor® 594

sc-508 AF594
200 µg/ml
$357.00

Phosphotyrosine 抗体 (PY20) Alexa Fluor® 647

sc-508 AF647
200 µg/ml
$357.00

Phosphotyrosine 抗体 (PY20) Alexa Fluor® 680

sc-508 AF680
200 µg/ml
$357.00

Phosphotyrosine 抗体 (PY20) Alexa Fluor® 790

sc-508 AF790
200 µg/ml
$357.00

Hello! Please tell me if p-Tyr Antibody (PY20): sc-508 is working in ELISA assay. If yes, could you recommend me a starting dilution range! I want to know if the p-Tyr Antibody (PY20) HRP variant is recommended for ELISA and in what dilution.

Asked by: Rodica
Thank you for your question. p-Tyr Antibody (PY20): sc-508 is recommended for detection of broad species by WB, IP, IF, IHC(P) and FCM. It is also available conjugated to HRP for ELISA. The starting dilution is 1:30, with a dilution range of 1:30-1:3000.
Answered by: Tech Support Europe
Date published: 2020-11-27

Which phosphotyrosine antibody is more sensitive in Western Blot: p-Tyr (PY99) or p-Tyr (PY20)? Which concentration is the recommended?

Asked by: AngelesMZ
Thank you for your question. Both p-Tyr Antibody (PY20): sc-508 and p-Tyr Antibody (PY99): sc-7020 are featured in several hundred publications and are recommended for western blot and other applications. Both antibodies have a recommended starting dilution of 1:200 and work in a dilution range 1:100-1:1000. Either antibody is perfectly suited for western blotting, so you could order one or the other or get in touch with our technical service to arrange for free samples of the two antibodies.
Answered by: Technical Support Europe
Date published: 2019-04-12

for blocking, what would you recommend? Many people recommend 5% of BSA for phospho-protein..( should I stick with this rule.). but some phospho-protein can worked well with.. Skim milk (depends the antibody protocol..)

Asked by: blessing
Thank you for your question. We recommend using 1% milk, 1% BSA in 0.05% Tween-20 for blocking and antibody incubations.
Answered by: Technical Support
Date published: 2019-02-01

EGF-treated とは、具体的にどのようにするものでしょうか。ご教授お願い致します。

Asked by: k 2018
Thank you for your question. Please contact our Technical Service Department and we will be happy to assist you. You may call us directly at 001 800-1338-3838, email at asia@scbio.cn, or contact us by live chat. お問い合わせを頂きまして、誠にありがとうございました。お手数ですが、電話 001 800-1338-3838、メールasia@scbio.cnまたはライブチャットで、当社のテクニカルサービスまでお問い合わせください。その他の不明点等がございましたら、またご連絡ください。
Answered by: Technical Support 8
Date published: 2018-12-21

Can I use p-Tyr Antibody (PY20): sc-508 for immunohistochemical staining of mouse tissue

Asked by: Anonymous
We recommend using a directly conjugated mouse monoclonal primary antibody. See p-Tyr (PY99): sc-7020 for p-Tyr antibody conjugates, including HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. https://www.scbt.com/scbt/product/p-tyr-antibody-py99
Answered by: Tech Service 6
Date published: 2016-12-16

What is the recommended secondary detection reagent in Western blot?

Asked by: Anonymous
We recommend our Mouse IgG Binding Protein conjugated to HRP as a secondary detection reagent: https://www.scbt.com/scbt/product/m-igg-kappa-bp-hrp A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 6
Date published: 2016-12-16
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Rated 5 out of 5 by from Always reliable!I have relied on you for over 20 years and you have always delivered an excellent product on time. Your phone staff is friendly and helpful.
Date published: 2018-03-08
Rated 4 out of 5 by from Very goodI have used Py20 Antibody from Santa and it worked great.
Date published: 2017-12-14
Rated 4 out of 5 by from As expectedGives WB bands as expected at 1:500 dilution in 5% SA (block in non-fat dry milk).
Date published: 2017-12-02
Rated 5 out of 5 by from Very Strong SignalI compare different products for art antibody found p-Tyr antibody is the most sensitive, sc-508 is the most reliable quality.
Date published: 2017-10-28
Rated 5 out of 5 by from Excellent antibodyStrong signal and low background on immunoprecipitated protein.
Date published: 2017-09-01
Rated 5 out of 5 by from good result antibodyIt is an excellent antibody. gives a single band in my hand.
Date published: 2017-05-27
Rated 5 out of 5 by from antibody for WBI use the dilution 1:800, get very clean bands.I like
Date published: 2017-05-07
Rated 5 out of 5 by from High QualityGreat antibody for detection of any protein with a phosphorylated Tyrosine, can be used in a number of applications, not species specific.
Date published: 2016-12-20
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Phosphotyrosine 抗体 (PY20) is rated 4.8 out of 5 by 19.
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