Date published: 2025-9-6
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p-MEK-1/2 Antibody (7E10) - Western Blotting - Image 136017
未经处理 (A, D), 血清饥饿, PMA 处理 (B, E), 血清饥饿, PMA 和 lambda 蛋白磷酸酶 (sc-200312A) 处理 (C, F) 的 HeLa 全细胞裂解液中 MEK-1 磷酸化的 Western 印迹分析. 测试的抗体包括 p-MEK-1/2 抗体 (7E10): sc-81503 (A, B, C) 和 MEK-1 (H-8): sc-6250 (D, E, F).
p-MEK-1/2 Antibody (7E10) - Western Blotting - Image 136017
未经处理 (A, D), 血清饥饿, PMA 处理 (B, E), 血清饥饿, PMA 和 lambda 蛋白磷酸酶 (sc-200312A) 处理 (C, F) 的 HeLa 全细胞裂解液中 MEK-1 磷酸化的 Western 印迹分析. 测试的抗体包括 p-MEK-1/2 抗体 (7E10): sc-81503 (A, B, C) 和 MEK-1 (H-8): sc-6250 (D, E, F).
p-MEK-1/2 Antibody (7E10) - Western Blotting - Image 127776
未经处理 (A, D), 经 PDGF 处理 (B, E) 以及经 PDGF 和 lambda 蛋白磷酸酶处理 (C, F) 的 NIH/3T3 全细胞裂解液中 MEK-1/2 磷酸化的 Western 印迹分析. 测试的抗体包括 p-MEK-1/2 抗体 (7E10): sc-81503 (A, B, C) 和 MEK-1 (H-8): sc-6250 (D, E, F).
p-MEK-1/2 Antibody (7E10) - Western Blotting - Image 62931
p-MEK-1/2 抗体 (7E10): sc-81503. 未处理 (A), EGF 刺激 (B) 和普伐那酸处理 (C) 的 A549 全细胞裂解液中 MEK-1/2 磷酸化的 Western 印迹分析.
未经处理 (A, D), 血清饥饿, PMA 处理 (B, E), 血清饥饿, PMA 和 lambda 蛋白磷酸酶 (sc-200312A) 处理 (C, F) 的 HeLa 全细胞裂解液中 MEK-1 磷酸化的 Western 印迹分析. 测试的抗体包括 p-MEK-1/2 抗体 (7E10): sc-81503 (A, B, C) 和 MEK-1 (H-8): sc-6250 (D, E, F).

p-MEK-1/2 抗体 (7E10): sc-81503

4.0(5)
寫評論提問

说明书
  • p-MEK-1/2 抗体 7E10 是小鼠单克隆 IgG1, 在44篇文献中引用，提供 50 µg/0.5 ml
  • 针对磷酸化肽段，与human起源的MEK-1/2的T-E-Y结构域周围的氨基酸残基相对应
  • 推荐用于 mouse, rat, human 和 canine 来源的Ser 218 and 222 phosphorylated MEK-1 and Ser 222 and 226 phosphorylated MEK-2 WB 和 IP检测
  • 关于如何获取p-MEK-1/2 (7E10): sc-81503的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是p-MEK-1/2 Antibody (7E10) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与p-MEK-1/2 Antibody (7E10) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

附加MEK抗体，包括MEK-1, MEK-2, MEK-3, MEK-4, MEK-5, MEK-6和MEK-7

p-MEK-1/2抗体（7E10）是一种IgG1 κ小鼠单克隆p-MEK-1/2抗体（也称为p-MEK-1/2抗体或MAP2K1、MAP2K2抗体），它通过蛋白质印迹法（WB）和免疫沉淀法（IP）检测小鼠、大鼠和人类来源的Ser 218和222磷酸化MEK-1以及Ser 222和226磷酸化MEK-2。p-MEK-1/2抗体（7E10）以非偶联形式的抗p-MEK-1/2抗体存在。已经鉴定出位于MAP激酶上游并负责其激活的一组蛋白激酶。该家族的原型成员被命名为MAP激酶激酶或MEK-1，它特异性地磷酸化MAP激酶调节性苏氨酸和酪氨酸残基，这些残基存在于ERK的Thr-Glu-Tyr模体中。第二个MEK家族成员MEK-2在底物特异性上与MEK-1相似。MEK-3（或MKK-3）的功能是激活p38 MAP激酶，而MEK-4（也称为SEK1或MKK-4）则激活p38和JNK MAP激酶。MEK-5似乎特异性地磷酸化ERK5，而MEK-6则磷酸化p38和p38β。MEK-7（或MKK-7）磷酸化并激活JNK信号转导途径。MAP激酶激酶激酶（RAF或MEKK1）对Ser/Thr的磷酸化正向调节激酶活性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

p-MEK-1/2 抗体 (7E10) 参考文献:

  1. 抑制MEK-1/2可防止N-甲酰肽挑战的人中性粒细胞中的5-脂氧合酶转位。  |  Boden, SE., et al. 2000. Int J Biochem Cell Biol. 32: 1069-74. PMID: 11091139
  2. MEK-1/2抑制可减少胎儿大鼠肺部的分支形态发生并导致间充质细胞凋亡。  |  Kling, DE., et al. 2002. Am J Physiol Lung Cell Mol Physiol. 282: L370-8. PMID: 11839529
  3. MEK-1/-2信号在单核细胞和粒细胞分化中的重要性。  |  Miranda, MB., et al. 2002. Leukemia. 16: 683-92. PMID: 11960350
  4. 血管紧张素II在肝细胞核中激活MEK 1/2和p42/44 MAPK，而乙醇则增强其活性。  |  Aroor, AR., et al. 2009. Alcohol. 43: 315-22. PMID: 19560630
  5. II期临床试验评估了塞鲁米替尼（AZD6244，ARRY-142886）--一种选择性MEK-1/2抑制剂在治疗复发性或持续性子宫内膜癌中的疗效：NRG肿瘤学/妇科肿瘤学小组的研究。  |  Coleman, RL., et al. 2015. Gynecol Oncol. 138: 30-5. PMID: 25887099
  6. MEK 1/2抑制剂在肝癌治疗中的应用。  |  Facciorusso, A., et al. 2015. Expert Rev Gastroenterol Hepatol. 9: 993-1003. PMID: 25915713
  7. Orexin-A通过PI3K/MEK1/2/ERK1/2信号通路保护SH-SY5Y细胞免受H2O2诱导的氧化损伤。  |  Wang, CM., et al. 2018. Int J Immunopathol Pharmacol. 32: 2058738418785739. PMID: 29983082
  8. 口服MEK 1/2抑制剂曲美替尼联合AKT抑制剂GSK2141795治疗急性髓系白血病伴RAS突变的患者：II期研究。  |  Ragon, BK., et al. 2019. Clin Lymphoma Myeloma Leuk. 19: 431-440.e13. PMID: 31056348
  9. 在雄激素缺乏症动物模型中，染料木素调节钙和磷酸盐稳态而不激活MEK 1/2信号通路。  |  Živanović, J., et al. 2022. Ann Anat. 239: 151836. PMID: 34563672
  10. MEK 1/2抑制剂PD98059与紫杉醇在体外具有协同抗子宫内膜癌活性，当与PAMAM包被的PLGA-PEG纳米颗粒配伍时，在体内可增强组织分布。  |  Wiwatchaitawee, K., et al. 2022. Drug Deliv Transl Res. 12: 1684-1696. PMID: 34635984

订购信息

产品名称产品编号规格价格数量收藏夹

p-MEK-1/2 抗体 (7E10)

sc-81503
50 µg/0.5 ml
$316.00

p-MEK-1/2 (7E10): m-IgG Fc BP-HRP 套装

sc-537247
50 µg Ab; 10 µg BP
$354.00

p-MEK-1/2 (7E10): m-IgGκ BP-HRP 套装

sc-534472
50 µg Ab; 40 µg BP
$354.00

p-MEK-1/2 (7E10): m-IgG1 BP-HRP 套装

sc-545103
50 µg Ab; 20 µg BP
$354.00

Hi I recently purchased this antibody, can I use this antibody for Immunofluorescence assay in cancer cell lines in vitro?

Asked by: Rodu
Thank you for your question. p-MEK-1/2 Antibody (7E10): sc-81503 is only recommended for detection of Ser 218 and 222 phosphorylated MEK-1 and Ser 222 and 226 phosphorylated MEK-2 of mouse, rat, human and canine origin by WB and IP. Based on our tests we do not recommend it for immunofluorescence.
Answered by: Tech Support Europe
Date published: 2024-06-21

Hi I need an option for IHC(P) for p-MEK-1/2 Antibody (7E10): sc-81503, any suggestions

Asked by: Parajuli
Thank your for your question. This antibody, sc-81503, is not recommended or warrantied for IHC(P). We do not currently offer a p-MEK-1/2 antibody recommended for this use. However, if you want to try it, you can find our protocol here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Tech Service
Date published: 2019-02-18

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 2
Date published: 2017-02-17
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Rated 3 out of 5 by from Not very intense bandsThe blot was quite unclear and the bands not very intense. I used A549 whole cell lysates and 60μg total protein. The antibody was incubated in milk 3% v/w
Date published: 2021-03-17
Rated 5 out of 5 by from Excellent!This antibody is recommended for detection of Ser 218 and 222 phosphorylated MEK-1 and Ser 222 and 226 phosphorylated MEK-2 of mouse, human and canine origin by WB and IP
Date published: 2017-01-20
Rated 4 out of 5 by from LightLight, positive band in WB in untreated and PDGF treated NIH/3T3 whole cell lysates. -SCBT QC
Date published: 2015-03-06
Rated 4 out of 5 by from GoodGood, clear band in WB in serum-starved, PMA-treated HeLa whole cell lysate. -SCBT QC
Date published: 2015-01-03
Rated 4 out of 5 by from Positive band seen in WB in EGF stimulatedPositive band seen in WB in EGF stimulated and pervanadate treated A549 whole cell lysate. -SCBT QC
Date published: 2014-07-28
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p-MEK-1/2 抗体 (7E10) is rated 4.0 out of 5 by 5.
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