Date published: 2025-9-8

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P-cadherin 抗体 (A-10): sc-74545

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说明书
  • P-cadherin 抗体 A-10 是小鼠单克隆 IgG1 κ,P-cadherin抗体, 在2篇文献中引用,规格为200 µg/ml
  • 与human的P-cadherin的细胞外结构域内的氨基酸550-654杂交
  • P-cadherin 抗体 (A-10) 推荐用于WB, IP, IF, IHC(P) 和 ELISA,检测human 来源的 P-cadherin
  • 抗P-cadherin抗体(A-10)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取P-cadherin (A-10): sc-74545免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 P-cadherin 抗体 (A-10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与P-cadherin 抗体 (A-10) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

P-cadherin抗体(A-10)是一种IgG1κ小鼠单克隆P-cadherin抗体(也称为Cadherin 3抗体或CDH3抗体),可通过WB、IP、IF、IHC(P)和ELISA检测人源P-cadherin蛋白。P-cadherin抗体(A-10)既可作为非偶联抗P-cadherin抗体形式提供,也可作为多种偶联形式的抗P-cadherin抗体提供,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。钙粘蛋白是一类依赖于Ca2+的粘附分子家族,它们在介导对维持组织结构和形态至关重要的细胞间结合中发挥作用。经典的钙粘蛋白E-、N-和P-钙粘蛋白由大的细胞外结构域组成,其特点是一系列五个同源的NH2末端重复序列。这些钙粘蛋白中最远端的被认为负责结合特异性、跨膜结构域和羧基末端细胞内结构域。相对较短的细胞内结构域与多种细胞质蛋白(如β-连环蛋白)相互作用,以调节钙粘蛋白的功能。该粘附蛋白家族的成员包括大鼠钙粘蛋白K(及其人类同源物钙粘蛋白-6)、R-钙粘蛋白、B-钙粘蛋白、E/P钙粘蛋白和钙粘蛋白-5。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

P-cadherin 抗体 (A-10)

sc-74545
200 µg/ml
$316.00

P-cadherin (A-10): m-IgG Fc BP-HRP 套装

sc-528687
200 µg Ab; 10 µg BP
$354.00

P-cadherin (A-10): m-IgGκ BP-HRP 套装

sc-521136
200 µg Ab, 40 µg BP
$354.00

P-cadherin (A-10): m-IgG1 BP-HRP 套装

sc-543066
200 µg Ab; 20 µg BP
$354.00

P-cadherin 抗体 (A-10) AC

sc-74545 AC
500 µg/ml, 25% agarose
$416.00

P-cadherin 抗体 (A-10) HRP

sc-74545 HRP
200 µg/ml
$316.00

P-cadherin 抗体 (A-10) FITC

sc-74545 FITC
200 µg/ml
$330.00

P-cadherin 抗体 (A-10) PE

sc-74545 PE
200 µg/ml
$343.00

P-cadherin 抗体 (A-10) Alexa Fluor® 488

sc-74545 AF488
200 µg/ml
$357.00

P-cadherin 抗体 (A-10) Alexa Fluor® 546

sc-74545 AF546
200 µg/ml
$357.00

P-cadherin 抗体 (A-10) Alexa Fluor® 594

sc-74545 AF594
200 µg/ml
$357.00

P-cadherin 抗体 (A-10) Alexa Fluor® 647

sc-74545 AF647
200 µg/ml
$357.00

P-cadherin 抗体 (A-10) Alexa Fluor® 680

sc-74545 AF680
200 µg/ml
$357.00

P-cadherin 抗体 (A-10) Alexa Fluor® 790

sc-74545 AF790
200 µg/ml
$357.00

I would like to use P-Cadherin-FITC (sc-74545 FITC) for flow sorting experiments. Does it work on trypsinized membranes? If not what can we use to harvest cells?

Asked by: Anonymous
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-22

I am having some trubles with paraffin IHC, I have tested the antibody sc-74545 in normal cervix and esophagus using the dilution 1:200 and 1:100, and the signal is very weak . Besides tonsil which other postive control do you recomend?

Asked by: Lucytch
Thank you for your question. P-Cadherin is expressed highly in lung, skin, bone marrow, and proximal digestive tract. Feel free to also reach out to our technical service team for troubleshooting advice.
Answered by: Tech Support Europe
Date published: 2020-11-19

Can the antibody be detected using the common secondary mouse antibody (give good signal) or does it have to be your recommended m-IgGκ BP-HRP? Thank you very much

Asked by: AHL2
Thanks for your question. Yes P-cadherin (A-10) can be better detected using our recommended mouse IgG kappa binding protein. Please contact technical service to find out which conjugated better suits to your needs.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

Can it be used for flow cytometry?

Asked by: Yanzi
Thank you for your question. This antibody has not been tested for Flow cytometry, so we do not recommend it for that application. It is possible that it work, but we don't have any information. Please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com if you would like to try a sample of it!
Answered by: Tech Service
Date published: 2017-10-26

Can P-cadherin (A-10): sc-74545 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Professor Griffin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, P-cadherin (A-10): sc-74545 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-25

Will this antibody work on pig samples? Thanks

Asked by: Dana
Thank you for your question. We do not recommend our P-cadherin antibody sc-74545 for detection of the pig protein.
Answered by: Technical Service
Date published: 2016-11-01
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Rated 4 out of 5 by from Works wellI have used the P-cadherin antibody for both Western blot (1:1000) as well as ICC (1:100) staining of HT29-19A cells and it works for both applications.
Date published: 2017-08-25
Rated 5 out of 5 by from Good specificity and signal strength in PCGood specificity and signal strength in PC-3, A-431 and T84 -SCBT QC
Date published: 2015-02-18
Rated 5 out of 5 by from Excellent antibody for western blotExcellent antibody for western blot. Specific bands detected with transfected lysate -SCBT QC
Date published: 2013-04-24
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P-cadherin 抗体 (A-10) is rated 4.7 out of 5 by 3.
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