Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Optineurin Double Nickase Plasmid (h): sc-401851-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Optineurin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Optineurin Double Nickase Plasmid (h) and Optineurin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting OPTN. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Optineurin Antibody (C-2): sc-166576
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Optineurin Double Nickase Plasmid (h) | sc-401851-NIC | 20 µg | $410.00 | |||
Optineurin Double Nickase Plasmid (h2) | sc-401851-NIC-2 | 20 µg | $410.00 |
OPTN encodes optineurin, a ubiquitin-binding adaptor that integrates selective autophagy, vesicular trafficking, and innate immune signaling. Optineurin functions as an autophagy receptor during xenophagy and mitophagy by linking ubiquitinated cargo to LC3 family proteins, and it modulates NF-κB and TBK1/IRF3-dependent antiviral pathways. It also contributes to Golgi maintenance and membrane dynamics through interactions with myosin VI, Rab GTPases, and ubiquitin chain complexes. Dysregulation or mutation of OPTN has been associated with neurodegeneration and glaucoma-related phenotypes, making it a widely studied node in proteostasis and inflammatory signaling networks.
Optineurin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the OPTN locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within OPTN. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt OPTN function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of OPTN-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.