NUDT8 Double Nickase Plasmid (m): sc-425987-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NUDT8 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NUDT8 Double Nickase Plasmid (m) and NUDT8 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Nudt8. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NUDT8 Double Nickase Plasmid (m) | sc-425987-NIC | 20 µg | $410.00 |
Nudt8 encodes NUDT8, a member of the Nudix hydrolase family implicated in sanitizing the cellular nucleotide pool by hydrolyzing potentially mutagenic or signaling-active nucleotide diphosphate derivatives. By regulating the abundance of oxidized or non-canonical nucleotides, NUDT8 can influence DNA replication fidelity, redox-associated stress responses, and nucleotide metabolism pathways that intersect with mitochondrial and peroxisomal homeostasis. Dysregulated nucleotide sanitization is broadly relevant to genome instability and inflammatory stress phenotypes, making Nudt8 a useful target for probing mechanisms that couple metabolic state to DNA damage susceptibility. Mouse Nudt8 studies can help clarify tissue-specific roles of Nudix enzymes in oxidative stress handling and nucleic acid quality control.
NUDT8 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Nudt8 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Nudt8. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Nudt8 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Nudt8-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.