Nucleoredoxin Antibody (E-12): sc-393748
- Nucleoredoxin Antibody (E-12) is a mouse monoclonal IgG2b κ Nucleoredoxin antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 125-189 mapping within an internal region of Nucleoredoxin of human origin
- Nucleoredoxin Antibody (E-12) is recommended for detection of Nucleoredoxin of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Nucleoredoxin Antibody (E-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Nucleoredoxin Antibody (E-12) for WB applications. These reagents are now offered in bundles with Nucleoredoxin Antibody (E-12) (see ordering information below).
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Nucleoredoxin Antibody (E-12) is a mouse monoclonal IgG2b kappa light chain antibody that detects Nucleoredoxin (NXN) in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Nucleoredoxin antibody (E-12) comes in non-conjugated form and various conjugates, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® options. Nucleoredoxin, also known as NRX or TRG-4, is a 435 amino acid protein found mainly in the cytoplasm and nucleus, functioning as a redox-dependent negative regulator of the Wnt signaling pathway. This regulation supports essential cellular processes, including cell proliferation, differentiation, and embryonic development. Nucleoredoxin shows widespread expression in adult tissues, with notable presence in the nervous system and limb buds during key embryonic stages (day 10.5-11.5). A conserved thioredoxin domain and catalytic motif enable oxidoreductase activity, suggesting involvement in transcriptional regulation and modulation of protein phosphatase 2A (PP2A-Cα) activity. During oxidative stress, Nucleoredoxin interacts with dishevelled 1 (Dvl) through PDZ domain binding, demonstrating significance in stress-related cellular signaling. Located on human chromosome 17p13.3 and mouse chromosome 11 B5, Nucleoredoxin maintains evolutionary conservation across species.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Nucleoredoxin Antibody (E-12) References:
- Nucleoredoxin, glutaredoxin, and thioredoxin differentially regulate NF-kappaB, AP-1, and CREB activation in HEK293 cells. | Hirota, K., et al. 2000. Biochem Biophys Res Commun. 274: 177-82. PMID: 10903915
- Interaction of nucleoredoxin with protein phosphatase 2A. | Lechward, K., et al. 2006. FEBS Lett. 580: 3631-7. PMID: 16764867
- Nucleoredoxin, a novel thioredoxin family member involved in cell growth and differentiation. | Funato, Y. and Miki, H. 2007. Antioxid Redox Signal. 9: 1035-57. PMID: 17567240
- Cloning and characterization of the nucleoredoxin gene that encodes a novel nuclear protein related to thioredoxin. | Kurooka, H., et al. 1997. Genomics. 39: 331-9. PMID: 9119370
- A novel nuclear member of the thioredoxin superfamily. | Laughner, BJ., et al. 1998. Plant Physiol. 118: 987-96. PMID: 9808743
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nucleoredoxin Antibody (E-12) | sc-393748 | 200 µg/ml | $322.00 | |||
Nucleoredoxin Antibody (E-12): m-IgG Fc BP-HRP Bundle | sc-530566 | 200 µg Ab; 10 µg BP | $361.00 | |||
Nucleoredoxin Antibody (E-12): m-IgGκ BP-HRP Bundle | sc-524072 | 200 µg Ab, 40 µg BP | $361.00 | |||
Nucleoredoxin Antibody (E-12): m-IgG2b BP-HRP Bundle | sc-550200 | 200 µg Ab; 10 µg BP | $361.00 | |||
Nucleoredoxin Antibody (E-12) AC | sc-393748 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Nucleoredoxin Antibody (E-12) HRP | sc-393748 HRP | 200 µg/ml | $322.00 | |||
Nucleoredoxin Antibody (E-12) FITC | sc-393748 FITC | 200 µg/ml | $336.00 | |||
Nucleoredoxin Antibody (E-12) PE | sc-393748 PE | 200 µg/ml | $349.00 | |||
Nucleoredoxin Antibody (E-12) Alexa Fluor® 488 | sc-393748 AF488 | 200 µg/ml | $364.00 | |||
Nucleoredoxin Antibody (E-12) Alexa Fluor® 546 | sc-393748 AF546 | 200 µg/ml | $364.00 | |||
Nucleoredoxin Antibody (E-12) Alexa Fluor® 594 | sc-393748 AF594 | 200 µg/ml | $364.00 | |||
Nucleoredoxin Antibody (E-12) Alexa Fluor® 647 | sc-393748 AF647 | 200 µg/ml | $364.00 | |||
Nucleoredoxin Antibody (E-12) Alexa Fluor® 680 | sc-393748 AF680 | 200 µg/ml | $364.00 | |||
Nucleoredoxin Antibody (E-12) Alexa Fluor® 790 | sc-393748 AF790 | 200 µg/ml | $364.00 |