NUBP1 Antibody (C-7): sc-514175

NUBP1 Antibody (C-7) is a mouse monoclonal IgG2b antibody that detects NUBP1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NUBP1, also known as cytosolic Fe-S cluster assembly factor NUBP1 or Nbp35, is a crucial 320 amino acid protein that plays a significant role in the regulation of centrosome duplication, which is vital for proper cell division and maintaining genomic stability. Anti-NUBP1 antibody (C-7) is a member of the Mrp/NBP35 ATP-binding protein family and exists in two alternatively spliced isoforms, allowing for functional diversity. NUBP1 is integral to the cytosolic iron-sulfur (Fe/S) protein assembly machinery, facilitating the transfer of iron-sulfur clusters to specific apoproteins, which is essential for the activity of various enzymes and proteins that rely on these clusters for their function. The gene encoding NUBP1 is located on human chromosome 16, a region associated with several genetic disorders, including giant axonal neuropathy and Rubinstein-Taybi syndrome, highlighting NUBP1′s importance in cellular health and disease. NUBP1 monoclonal antibody (C-7) serves as a valuable tool for researchers studying the intricate mechanisms of centrosome regulation and iron-sulfur cluster assembly, providing insights into both normal cellular processes and pathological conditions.

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NUBP1 Antibody (C-7) References:

1. Two novel mouse genes--Nubp2, mapped to the t-complex on chromosome 17, and Nubp1, mapped to chromosome 16--establish a new gene family of nucleotide-binding proteins in eukaryotes.  |  Nakashima, H., et al. 1999. Genomics. 60: 152-60. PMID: 10486206
2. Motor protein KIFC5A interacts with Nubp1 and Nubp2, and is implicated in the regulation of centrosome duplication.  |  Christodoulou, A., et al. 2006. J Cell Sci. 119: 2035-47. PMID: 16638812
3. Comparison of intracellular localization of Nubp1 and Nubp2 using GFP fusion proteins.  |  Okuno, T., et al. 2010. Mol Biol Rep. 37: 1165-8. PMID: 19263241
4. Nubp1 is required for lung branching morphogenesis and distal progenitor cell survival in mice.  |  Schnatwinkel, C. and Niswander, L. 2012. PLoS One. 7: e44871. PMID: 23028652
5. The nucleotide-binding proteins Nubp1 and Nubp2 are negative regulators of ciliogenesis.  |  Kypri, E., et al. 2014. Cell Mol Life Sci. 71: 517-38. PMID: 23807208
6. A novel family of katanin-like 2 protein isoforms (KATNAL2), interacting with nucleotide-binding proteins Nubp1 and Nubp2, are key regulators of different MT-based processes in mammalian cells.  |  Ververis, A., et al. 2016. Cell Mol Life Sci. 73: 163-84. PMID: 26153462
7. Expression of family with sequence similarity 172 member A and nucleotide-binding protein 1 is associated with the poor prognosis of colorectal carcinoma.  |  Liu, W., et al. 2017. Oncol Lett. 14: 3587-3593. PMID: 28927116
8. GLRX3 Acts as a [2Fe-2S] Cluster Chaperone in the Cytosolic Iron-Sulfur Assembly Machinery Transferring [2Fe-2S] Clusters to NUBP1.  |  Camponeschi, F., et al. 2020. J Am Chem Soc. 142: 10794-10805. PMID: 32429669
9. Proteomic analysis of host cellular proteins co-immunoprecipitated with duck enteritis virus gC.  |  Chen, L., et al. 2021. J Proteomics. 245: 104281. PMID: 34091090
10. Unraveling the mechanism of [4Fe-4S] cluster assembly on the N-terminal cluster binding site of NUBP1.  |  Bargagna, B., et al. 2023. Protein Sci. 32: e4625. PMID: 36916754

The preferred NUBP1 antibody is NUBP1 Antibody (C-3): sc-398368.