Date published: 2025-9-7
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Nrl Antibody (F-2) - Western Blotting - Image 300494
Nrl 抗体 (F-2): sc-374277. Y79 (A) 和 ARPE-19 (B) 全细胞裂解液中 Nrl 表达的 Western 印迹分析.
Nrl Antibody (F-2) - Western Blotting - Image 300494
Nrl 抗体 (F-2): sc-374277. Y79 (A) 和 ARPE-19 (B) 全细胞裂解液中 Nrl 表达的 Western 印迹分析.
Nrl Antibody (F-2) - Western Blotting - Image 149636
Nrl 抗体 (F-2): sc-374277. 非转染: sc-117752 (A) 和人 Nrl 转染: sc-113074 (B) 293T 全细胞裂解液中 Nrl 表达的 Western 印迹分析.
Nrl 抗体 (F-2): sc-374277. Y79 (A) 和 ARPE-19 (B) 全细胞裂解液中 Nrl 表达的 Western 印迹分析.

Nrl 抗体 (F-2): sc-374277

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寫評論提問

说明书
  • Nrl 抗体 F-2 是小鼠单克隆 IgG1 κ，Nrl抗体, 在5篇文献中引用，规格为200 µg/ml
  • 针对human起源的Nrl内部区域内的氨基酸21-140进行
  • Nrl 抗体 (F-2) 推荐用于WB, IP, IF 和 ELISA，检测human 来源的 Nrl
  • 抗Nrl抗体（F-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于ChIP的TransCruz试剂(sc-374277 X, 200 µg/0.1 ml)
  • 关于如何获取Nrl (F-2): sc-374277的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成Nrl 抗体 (F-2)的首选二抗检测试剂的鉴定。这项工作正在进行中。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

Nrl抗体（F-2）是一种IgG1κ小鼠单克隆Nrl抗体（也称为Nrl抗体），可通过WB、IP、IF和ELISA检测人源Nrl蛋白。Nrl抗体（F-2）有非偶联抗Nrl抗体形式和多种偶联抗Nrl抗体形式，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Nrl（神经视网膜亮氨酸拉链）是转录因子Maf家族的成员，其特征是含有高度保守的碱性亮氨酸拉链（bZIP）-DNA结合基序。Nrl和c-Maf优先结合T-MARE位点，并涉及多种发育和生理作用。Maf-Nrl亚家族调节造血系统、小脑和发育后脑组织中细胞类型特异性基因的表达。Maf和Nrl蛋白结合一个延伸的AP-1样序列，可以与Fos和Jun转录因子形成异二聚体。在视网膜细胞和感光细胞中，Nrl通过与视紫红质启动子中存在的Nrl反应元件结合来促进视紫红质的表达。在神经元分化过程中，Nrl在整个发育中的中枢和周围神经系统中表达，在成年期，其表达仅限于新皮质、脑干和视网膜神经元。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Nrl 抗体 (F-2) 参考文献:

  1. 一个保守的视网膜特异基因编码一个基本图案/亮氨酸拉链结构域。  |  Swaroop, A., et al. 1992. Proc Natl Acad Sci U S A. 89: 266-70. PMID: 1729696
  2. bZIP 蛋白 NRL 中的视网膜病变突变改变了磷酸化和转录活性。  |  Kanda, A., et al. 2007. Hum Mutat. 28: 589-98. PMID: 17335001
  3. Nr2e3和Nrl可将爪蟾视网膜前体重编程为视杆细胞。  |  McIlvain, VA. and Knox, BE. 2007. Dev Dyn. 236: 1970-9. PMID: 17377979
  4. maf 原癌基因刺激启动子中多个位点的转录，从而引导浦肯野神经元特异性基因的表达。  |  Kurschner, C. and Morgan, JI. 1995. Mol Cell Biol. 15: 246-54. PMID: 7799931
  5. Maf/Nrl 家族蛋白识别扩展 DNA 结合位点需要邻近基本结构域的保守区域。  |  Kerppola, TK. and Curran, T. 1994. Oncogene. 9: 3149-58. PMID: 7936637
  6. Maf 和 Nrl 可与 AP-1 位点结合，并与 Fos 和 Jun 形成异二聚体。  |  Kerppola, TK. and Curran, T. 1994. Oncogene. 9: 675-84. PMID: 8108109
  7. 红细胞转录因子NF-E2的泛在亚基是一种与v-maf癌基因有关的碱性亮氨酸拉链小蛋白。  |  Andrews, NC., et al. 1993. Proc Natl Acad Sci U S A. 90: 11488-92. PMID: 8265578
  8. 发育中神经系统中 bZIP 转录因子基因 Nrl 的表达。  |  Liu, Q., et al. 1996. Oncogene. 12: 207-11. PMID: 8552394
  9. bZIP 转录因子 Nrl 可刺激原代视网膜细胞培养物中的视网膜素启动子活性。  |  Kumar, R., et al. 1996. J Biol Chem. 271: 29612-8. PMID: 8939891

订购信息

产品名称产品编号规格价格数量收藏夹

Nrl 抗体 (F-2)

sc-374277
200 µg/ml
$316.00

Nrl 抗体 (F-2) X

sc-374277 X
200 µg/0.1 ml
$316.00

Nrl 抗体 (F-2) AC

sc-374277 AC
500 µg/ml, 25% agarose
$416.00

Nrl 抗体 (F-2) HRP

sc-374277 HRP
200 µg/ml
$316.00

Nrl 抗体 (F-2) FITC

sc-374277 FITC
200 µg/ml
$330.00

Nrl 抗体 (F-2) PE

sc-374277 PE
200 µg/ml
$343.00

Nrl 抗体 (F-2) Alexa Fluor® 488

sc-374277 AF488
200 µg/ml
$357.00

Nrl 抗体 (F-2) Alexa Fluor® 546

sc-374277 AF546
200 µg/ml
$357.00

Nrl 抗体 (F-2) Alexa Fluor® 594

sc-374277 AF594
200 µg/ml
$357.00

Nrl 抗体 (F-2) Alexa Fluor® 647

sc-374277 AF647
200 µg/ml
$357.00

Nrl 抗体 (F-2) Alexa Fluor® 680

sc-374277 AF680
200 µg/ml
$357.00

Nrl 抗体 (F-2) Alexa Fluor® 790

sc-374277 AF790
200 µg/ml
$357.00

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with Nrl (F-2): sc-374277?

Asked by: AbPolly
We recommend using one of our our exclusive Mouse IgG Binding Proteins conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support
Date published: 2017-02-27
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Rated 1 out of 5 by from Nrl F-2 antibody IF on mouse retinaI have tested Nrl "F2" antibody on cryostat and paraffin-embedded sections of 1 month-old C57Bl/6 and Nrl -/- retina at dilutions from 1:25 to 1:1000, with or without antigen retrieval. I see no reactivity in outer nuclear layer cells under any conditions tested, although inner segments show slight reactivity. Details: antibody lot #D2116; antigen retrieval 10 min at 95-100 degrees C (paraffin) or overnight at 60 degrees C (cryosections); blocked in 10% goat serum; primary antibody incubation overnight at 4 degrees C (paraffin and cryosections) or 2 hours at RT (cryostat sections); Alexa-488-coupled goat anti-mouse secondary antibody incubation 2 hours at RT.
Date published: 2017-02-28
Rated 5 out of 5 by from clear protein band ,good for WBI use this antibody to detect the expression of Nrl of Mesenchymal stem cells after certain neural induction . The following procedures were conducted with WB 1The cells were lysated with RIPA, PMSF, and proteinase cocktail ,then ultrasound 2 electrophoresis, transfer to NC membrane 3 incubated with first antibody( sc-374277 )is diluted at:1:500 ,4 °C,overnight 4 washed, incubated with goat anti-mouse HRP conjugate second antibody, ECL coloration 5 results are satisfactory,the protein band is clear 26KDA
Date published: 2017-01-19
Rated 5 out of 5 by from Produced positive Western Blot data of NrlProduced positive Western Blot data of Nrl expression in Y79 and ARPE-19 whole cell lysates. -SCBT QC
Date published: 2015-02-18
Rated 5 out of 5 by from Produced positive Western Blot data of NrlProduced positive Western Blot data of Nrl expression in non-transfected and human Nrl transfected 293T whole cell lysates. -SCBT QC
Date published: 2014-12-07
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Nrl 抗体 (F-2) is rated 4.0 out of 5 by 4.
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