Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Notch 3 CRISPR/Cas9 KO Plasmid (m): sc-421933
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Notch 3 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Notch 3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Notch 3 Antibody (A-6): sc-515825
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Notch 3 CRISPR/Cas9 KO Plasmid (m) | sc-421933 | 20 µg | $397.00 |
Overview
Notch3 encodes the Notch 3 receptor, a single-pass transmembrane protein that mediates juxtacrine signaling to control cell fate decisions, proliferation, and differentiation in a context-dependent manner. Upon ligand engagement, receptor processing enables release of the Notch intracellular domain, which translocates to the nucleus and regulates transcriptional programs with CSL/RBPJ and co-activators. In mouse tissues, Notch3 is prominently linked to vascular smooth muscle/pericyte biology and contributes to vessel maturation, tissue homeostasis, and stress responses. Dysregulated Notch3 signaling has been associated with aberrant differentiation states and vascular pathology, making it a relevant node for studying Notch pathway crosstalk and lineage control.
Notch 3 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Notch3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Notch3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Notch3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Notch 3 protein expression.
This CRISPR knockout system enables efficient generation of Notch3-deficient cell models for investigation of Notch 3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Notch3 exon(s) critical for Notch 3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Notch3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Notch 3 CRISPR/Cas9 KO Plasmid (m) and Notch 3 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Notch3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Notch 3 HDR Plasmid (m) and Notch 3 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Notch3 homology arms to support homology-directed repair at defined Notch3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.