NOP2 Lentiviral Activation Particles (h): sc-409656-LAC

Human NOP2 encodes a nucleolar RNA methyltransferase implicated in ribosome biogenesis through site-specific modification of rRNA and coordination of pre-rRNA processing. NOP2 activity supports nucleolar organization, cell-cycle progression, and translational capacity, linking it to growth-control programs and cellular stress responses that converge on RNA metabolism. Dysregulated NOP2 expression is frequently studied in the context of proliferative states, where altered ribosome production can reshape proteome output and genome maintenance pathways. As a result, NOP2 is a useful target for investigating nucleolar function, rRNA modification–dependent translation, and RNA-centric mechanisms associated with oncogenic phenotypes.

NOP2 Lentiviral Activation Particles (h) address this need by packaging the complete synergistic activation mediator (SAM) transcriptional activation system into transduction-ready, high-titer lentiviral particles, enabling efficient NOP2 upregulation across a broader range of human cell types.

NOP2 Lentiviral Activation Particles (h) deliver all functional components of the synergistic activation mediator (SAM) system via lentiviral transduction. The system comprises three particle preparations co-transduced into target cells: one encoding catalytically inactive dCas9 (D10A and N863A mutations) fused to the VP64 transactivation domain with a blasticidin resistance gene; one encoding the MS2-p65-HSF1 fusion protein with a hygromycin resistance gene; and one encoding a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers with a puromycin resistance gene. Following lentiviral transduction and genomic integration of the expression cassettes, the SAM components are stably expressed and assemble at the target locus within the proximal promoter region upstream of the NOP2 transcriptional start site, where VP64, p65, and HSF1 act cooperatively to recruit endogenous transcriptional machinery and drive sustained upregulation of endogenous NOP2 expression. The use of nuclease-inactive dCas9 avoids the introduction of double-strand DNA breaks and preserves the native NOP2 genomic locus and regulatory architecture.

The lentiviral format offers several practical advantages: stable genomic integration supports heritable activation across cell divisions; high-titer particle preparations eliminate the need for in-house viral production; and compatibility with primary, non-dividing, and transfection-resistant cell types expands experimental accessibility. Successful transduction can be confirmed and enriched through triple antibiotic selection using puromycin, hygromycin, and blasticidin.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.