Date published: 2025-9-23
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NFκB p50 Antibody (E-10) - Immunohistochemistry - Image 156586
NFκB p50 (E-10): sc-8414. 福尔马林固定, 石蜡包埋的人类输卵管组织的免疫过氧化物酶染色显示腺细胞的胞浆染色.
NFκB p50 (E-10): sc-8414. 福尔马林固定, 石蜡包埋的人类输卵管组织的免疫过氧化物酶染色显示腺细胞的胞浆染色.

NFκB p50 抗体 (E-10): sc-8414

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说明书
  • NF kappa B p50 抗体 E-10 是小鼠单克隆 IgG1 κ，NFκB p50抗体, 在371篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列120-239位于human物种的NFκB p50的N-terminus
  • NF kappa B p50 抗体 (E-10) 推荐用于 WB, IP, IF, IHC(P), FCM 和 ELISA，检测mouse, rat 和human 来源的 NFκB p50 and p105
  • 抗NF kappa B p50抗体（E-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联Alexa Fluor® 405 用于IF, IHC(P) 和FCM
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-8414 X, 200 µg/0.1 ml)
  • 关于如何获取NFκB p50 (E-10): sc-8414的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 NFκB p50 抗体 (E-10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与NFκB p50 抗体 (E-10) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 NF κ B 抗体 包括 NF κ B p50, NF κ B p52和NF κ B p65

NFκB p50抗体（E-10）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过多种应用检测小鼠、大鼠和人类来源的NFκB p50蛋白，包括蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学（IHC）、流式细胞术（FCM）和酶联免疫吸附测定（ELISA）。NFκB p50 (E-10) 抗体有非结合型和多种结合型，如琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种 Alexa Fluor® 结合物。NFκB p50蛋白在调节免疫反应和炎症方面发挥着关键作用，它作为一种转录因子，控制着与这些过程相关的基因的表达。NFκB p50蛋白在细胞分裂信号的作用下从细胞质转移到细胞核，这对于激活免疫细胞、维持正常的免疫功能和病原体反应至关重要。NFκB p50亚基由前体蛋白p105衍生而来，其中包含一个调节域，可调节NFκB p50的活性，凸显了NFκB信号通路复杂的控制机制。了解NFκB p50的功能对于阐明其在包括癌症和自身免疫性疾病在内的各种疾病中的作用非常重要，因为该通路失调会导致病理状况。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

NFκB p50 抗体 (E-10) 参考文献:

  1. 可逆乙酰化调节核 NF-kappaB 作用的持续时间。  |  Chen Lf,., et al. 2001. Science. 293: 1653-7. PMID: 11533489
  2. NF-kappa B 前体 p105 和原癌基因 bcl-3 的杏仁蛋白重复结构域是 NF-kappa B DNA 结合的特异性抑制剂。  |  Hatada, EN., et al. 1992. Proc Natl Acad Sci U S A. 89: 2489-93. PMID: 1532257
  3. 人类 NF-kappa B 的 65 kDa 亚基是一种强效转录激活剂，也是 v-Rel 介导的抑制作用的靶标。  |  Ballard, DW., et al. 1992. Proc Natl Acad Sci U S A. 89: 1875-9. PMID: 1542686
  4. p50 NF-kappa B 亚基前体中核定位信号和二聚化结构域的分子内屏蔽。  |  Henkel, T., et al. 1992. Cell. 68: 1121-33. PMID: 1547506
  5. 原代乳腺上皮细胞在肿瘤坏死因子刺激下的生长不需要 NFkappaB1/p50：NFkappaB2/p52 和 RelB 的影响。  |  Zhang, J., et al. 2007. Endocrinology. 148: 268-78. PMID: 17008396
  6. 克隆与 p65 协同刺激 HIV 转录的 NF-kappa B 亚基。  |  Schmid, RM., et al. 1991. Nature. 352: 733-6. PMID: 1876189
  7. NF-kappaB1（p50）的生物功能及其作为抗癌靶点的潜力  |  Yu, Y., et al. 2009. Curr Cancer Drug Targets. 9: 566-71. PMID: 19519322
  8. 克隆人类核因子卡巴 B 的 DNA 结合亚基：其 mRNA 水平受植物醇酯或肿瘤坏死因子 alpha 的强烈调控。  |  Meyer, R., et al. 1991. Proc Natl Acad Sci U S A. 88: 966-70. PMID: 1992489
  9. NF-κB p50 功能的缺失会协同增强中年大脑中的小胶质细胞引物。  |  Taetzsch, T., et al. 2019. J Neuroinflammation. 16: 60. PMID: 30871598
  10. 新型 STAT3/ NFκB p50 轴调节基质-KDM2A，促进乳腺癌中 M2 巨噬细胞介导的化疗抵抗。  |  Chen, JS., et al. 2023. Cancer Cell Int. 23: 237. PMID: 37821959
  11. 缺乏 NF-kappa B 的 RelA 成分的小鼠的胚胎致死率和肝脏退化。  |  Beg, AA., et al. 1995. Nature. 376: 167-70. PMID: 7603567
  12. IkappaBalpha/NF-kappaB 复合物的晶体结构揭示了 NF-kappaB 失活的机制。  |  Huxford, T., et al. 1998. Cell. 95: 759-70. PMID: 9865694

订购信息

产品名称产品编号规格价格数量收藏夹

NFκB p50 抗体 (E-10)

sc-8414
200 µg/ml
$316.00

NFκB p50 (E-10): m-IgG Fc BP-HRP 套装

sc-526519
200 µg Ab; 10 µg BP
$354.00

NFκB p50 (E-10): m-IgG1 BP-HRP 套装

sc-531892
200 µg Ab; 20 µg BP
$354.00

NFκB p50 抗体 (E-10) X

sc-8414 X
200 µg/0.1 ml
$316.00

NFκB p50 抗体 (E-10) AC

sc-8414 AC
500 µg/ml, 25% agarose
$416.00

NFκB p50 抗体 (E-10) HRP

sc-8414 HRP
200 µg/ml
$316.00

NFκB p50 抗体 (E-10) FITC

sc-8414 FITC
200 µg/ml
$330.00

NFκB p50 抗体 (E-10) PE

sc-8414 PE
200 µg/ml
$343.00

NFκB p50 抗体 (E-10) Alexa Fluor® 488

sc-8414 AF488
200 µg/ml
$357.00

NFκB p50 抗体 (E-10) Alexa Fluor® 546

sc-8414 AF546
200 µg/ml
$357.00

NFκB p50 抗体 (E-10) Alexa Fluor® 594

sc-8414 AF594
200 µg/ml
$357.00

NFκB p50 抗体 (E-10) Alexa Fluor® 647

sc-8414 AF647
200 µg/ml
$357.00

NFκB p50 抗体 (E-10) Alexa Fluor® 680

sc-8414 AF680
200 µg/ml
$357.00

NFκB p50 抗体 (E-10) Alexa Fluor® 790

sc-8414 AF790
200 µg/ml
$357.00

NFκB p50 抗体 (E-10) Alexa Fluor® 405

sc-8414 AF405
200 µg/ml
$357.00

which membrane type is proper for this antibody, should I prefer PVDF or nitrocellulose or doesn't matter (assume diluted in skimmed milk)

Asked by: Anonymous
Thank you for your question. This antibody is suitable for both PVDF and nitrocellulose membranes.
Answered by: Technical Support Europe
Date published: 2020-10-28

Does anyone know where to find an anti-p50 antibody conjugated to agarose or sepharose beads for IP?

Asked by: Kevin1
Thank you for your question. This antibody, NFκB p50 (E-10), is currently available conjugated to agarose (sepharose beads) for use in IP. The specific catalog number is: sc-8414 AC. We are in the process of adding this conjugated antibody to our website for you to place the order. In the meantime, please contact us directly by phone, fax or e-mail to place an order. Please call us toll-free at (800) 457-3801 option 1, or e-mail <customercare@scbt.com>.
Answered by: Technical Support
Date published: 2017-07-18

Will this antibody work with bovine tissue samples?

Asked by: Trav11
For additional species reactivity not listed on our website, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website and we will be more than happy to run sequence analysis for your species of interest.
Answered by: Tech Service 8
Date published: 2016-12-30

hi, i need antibody mouse p50 ab which will go wih all application-wb, ihc, ip, chip, flow, can i get in one ??

Asked by: SHILPI
Our mouse monoclonal antibody, NFκB p50 Antibody (E-10): sc-8414, is suitable for detection of mouse p50 and p105 in a broad range of applications, including: WB, IP, IF, IHC(P), FCM, ChIP and ELISA . We also offer fluorescent conjugated forms of this antibody which are recommended for use in FCM, including: phycoerythrin (sc-8414 PE) or fluoroscein (sc-8414 FITC), Alexa Fluor® 488 (sc-8414 AF488) or Alexa Fluor® 647 (sc-8414 AF647), 200 µg/ml. And a more concentrated form of the antibody, TransCruz reagent for Gel Supershift and ChIP applications (sc-8414 X, 200 µg/0.1 ml).
Answered by: Technical Support
Date published: 2016-09-20
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Rated 4 out of 5 by from The p50 antibody worked wellThe p50 antibody worked well in WB analysis of human samples
Date published: 2024-09-17
Rated 5 out of 5 by from Good antibody for detecting NFκB p50Excellent antibody for detecting NFκB p50 in ovine lymph node. Works at a dilution of 1:1000.
Date published: 2023-03-17
Rated 5 out of 5 by from Works greatIt works great in the liver for the detection of Anti-NFκB p50 using western blot , and has broad range or reactivity and great specificity, including sheep.
Date published: 2022-10-17
Rated 4 out of 5 by from Buon funzionamento in WBUsati su estratti cellulari, buon funzionamento in WB.
Date published: 2022-10-05
Rated 4 out of 5 by from Buon funzionamentoHo usato l'anticorpo su linee di fegato umane e la risposta è stata molto specifica, con un segnale al peso molecolare indicato.
Date published: 2022-03-24
Rated 5 out of 5 by from Works very well for western blottingWorks at a dilution of 1:500 on neonatal mouse lungs
Date published: 2021-05-07
Rated 4 out of 5 by from good antibodythe antibody works good in mice liver lysates, dilution 1:500
Date published: 2018-09-05
Rated 5 out of 5 by from 条带清晰条带清晰，就是50kda位置条带偏弱，其余还可以，稀释比试1：200，卵巢癌细胞裂解液为样品，下次牛奶稀释抗体尝试
Date published: 2018-03-28
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NFκB p50 抗体 (E-10) is rated 4.5 out of 5 by 47.
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