Date published: 2025-9-7

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netrin-4 抗体 (A-7): sc-365280

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说明书
  • netrin-4 抗体 A-7 是小鼠单克隆 IgG1 κ,netrin-4抗体, 在3篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列399-628位于human物种的netrin-4的C-terminus
  • netrin-4 抗体 (A-7) 推荐用于WB, IP, IF, IHC(P) 和 ELISA,检测human 来源的 netrin-4
  • 抗netrin-4抗体(A-7)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取netrin-4 (A-7): sc-365280免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 netrin-4 抗体 (A-7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与netrin-4 抗体 (A-7) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

netrin-4抗体(A-7)是一种IgG1 κ鼠单克隆netrin-4抗体(也被称为Beta-netrin抗体或NTN4抗体),它通过WB、IP、IF、IHC(P)和ELISA方法检测人类的netrin-4蛋白。netrin-4抗体(A-7)既可以作为非共轭抗netrin-4抗体形式提供,也可以作为多种共轭形式的抗netrin-4抗体提供,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。Netrin蛋白是一类层粘连蛋白相关的分泌蛋白,在发育过程中为轴突生长和细胞迁移提供指导信号。Netrin信号依赖于细胞外的钙浓度和PKA活性水平。在轴突细胞中,PKA活性的降低会改变轴突对netrin蛋白的反应性,因为细胞会被netrin梯度排斥,而不是被吸引。Netrin-4与层粘连蛋白β链相关,因此也被称为β-netrin。它存在于血管、外侧嗅束、肾脏和卵巢的基底膜中。在人类中,编码netrin-4蛋白的基因定位于染色体12q22-q23。在许多细胞和组织中,包括大脑皮层、海马体、杏仁核和浦肯野细胞中,也检测到了高水平的netrin-4 mRNA。Netrin-4在神经、肾脏和血管发育中具有重要意义。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

netrin-4 抗体 (A-7) 参考文献:

  1. Netrin-3是人类NTN2L的小鼠同源物,在感觉神经节中高度表达,并显示出与netrin受体的不同结合。  |  Wang, H., et al. 1999. J Neurosci. 19: 4938-47. PMID: 10366627
  2. 网状蛋白家族的新成员--β-网状蛋白与层粘连蛋白的β链具有同源性:鉴定, 表达和功能表征。  |  Koch, M., et al. 2000. J Cell Biol. 151: 221-34. PMID: 11038171
  3. Netrins在肺分支形态发生过程中调节上皮行为的新作用  |  Liu, Y., et al. 2004. Curr Biol. 14: 897-905. PMID: 15186747
  4. 鉴定人类净蛋白-4的一种新型替代剪接形式并分析其在成年大鼠大脑中的表达模式。  |  Zhang, C., et al. 2004. Brain Res Mol Brain Res. 130: 68-80. PMID: 15519678
  5. Netrin-4促进成骨细胞的分化和迁移。  |  Enoki, Y., et al. 2017. In Vivo. 31: 793-799. PMID: 28882944
  6. 人内皮细胞表达Netrin-4可抑制内皮炎症和衰老。  |  Zhang, H., et al. 2021. Int J Biochem Cell Biol. 134: 105960. PMID: 33636396
  7. Netrin-4在糖尿病视网膜病变中的抗炎作用。  |  Crespo-Garcia, S., et al. 2021. Int J Mol Sci. 22: PMID: 33923095
  8. Netrin-4:关注其在轴突引导、组织稳定性、血管生成和肿瘤中的作用。  |  Dong, F., et al. 2023. Cell Mol Neurobiol. 43: 1663-1683. PMID: 36350538
  9. 母体血清中Netrin-1和Netrin-4的水平与胎盘植入谱的关系。  |  Kaya, S., et al. 2023. Eur Rev Med Pharmacol Sci. 27: 4594-4600. PMID: 37259741
  10. 卫星细胞来源的肌细胞合成的Netrin-4可刺激自主融合。  |  Maeno, T., et al. 2023. Exp Cell Res. 430: 113698. PMID: 37437770
  11. Netrins是胚胎脊髓神经轴突的可扩散趋化因子。  |  Kennedy, TE., et al. 1994. Cell. 78: 425-35. PMID: 8062385
  12. 编码一种新型人类净蛋白的 NTN2L 基因映射到染色体 16p13.3 上的常染色体显性多囊肾病区域。  |  Van Raay, TJ., et al. 1997. Genomics. 41: 279-82. PMID: 9143507

订购信息

产品名称产品编号规格价格数量收藏夹

netrin-4 抗体 (A-7)

sc-365280
200 µg/ml
$316.00

netrin-4 (A-7): m-IgG Fc BP-HRP 套装

sc-527346
200 µg Ab; 10 µg BP
$354.00

netrin-4 (A-7): m-IgG1 BP-HRP 套装

sc-532719
200 µg Ab; 20 µg BP
$354.00

netrin-4 抗体 (A-7) AC

sc-365280 AC
500 µg/ml, 25% agarose
$416.00

netrin-4 抗体 (A-7) HRP

sc-365280 HRP
200 µg/ml
$316.00

netrin-4 抗体 (A-7) FITC

sc-365280 FITC
200 µg/ml
$330.00

netrin-4 抗体 (A-7) PE

sc-365280 PE
200 µg/ml
$343.00

netrin-4 抗体 (A-7) Alexa Fluor® 488

sc-365280 AF488
200 µg/ml
$357.00

netrin-4 抗体 (A-7) Alexa Fluor® 546

sc-365280 AF546
200 µg/ml
$357.00

netrin-4 抗体 (A-7) Alexa Fluor® 594

sc-365280 AF594
200 µg/ml
$357.00

netrin-4 抗体 (A-7) Alexa Fluor® 647

sc-365280 AF647
200 µg/ml
$357.00

netrin-4 抗体 (A-7) Alexa Fluor® 680

sc-365280 AF680
200 µg/ml
$357.00

netrin-4 抗体 (A-7) Alexa Fluor® 790

sc-365280 AF790
200 µg/ml
$357.00

I am using netrin-4 (A-7): sc-365280 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Professor Griffin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from The baseband is perfectWe found netrin-4 antibodies were expressed in human urinary bladder tissue and 1:2000 dilution could still yield very positive signals.
Date published: 2017-10-25
Rated 5 out of 5 by from ,perfect resultGood IF and WB antibody for mouse liver cells detction,perfect result.
Date published: 2017-02-11
Rated 5 out of 5 by from Recommended use.netrin-4 Antibody is the best I've ever used. It provides clear and specific results. Recommended use.
Date published: 2017-02-08
Rated 5 out of 5 by from perfect resultGood IF and WB antibody for mouse Microvascular endothelial cells detction,perfect result
Date published: 2017-01-17
Rated 5 out of 5 by from The source of the netrin - 4 WB inspection methodAntibodies for protein sample was human breast cancer cell lysate. The source of the netrin - 4 WB inspection method
Date published: 2017-01-16
Rated 5 out of 5 by from In the process of experiment,In the process of experiment, our whole team found positive immunoblot human recombinant netrin - 4 full length data, the data is really amazing.
Date published: 2017-01-12
Rated 5 out of 5 by from Superb immunoperoxidase extracellular stainingSuperb immunoperoxidase extracellular staining in formalin fixed, paraffin-embedded human urinary bladder tissue. -SCBT QC
Date published: 2015-01-17
Rated 5 out of 5 by from Produced positive Western Blot data of fullProduced positive Western Blot data of full length human recombinant netrin-4. -SCBT QC
Date published: 2013-07-07
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netrin-4 抗体 (A-7) is rated 5.0 out of 5 by 8.
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