Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Nek2 Double Nickase Plasmid (m): sc-421853-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Nek2 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Nek2 Double Nickase Plasmid (m) and Nek2 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Nek2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Nek2 Antibody (D-8): sc-55601
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nek2 Double Nickase Plasmid (m) | sc-421853-NIC | 20 µg | $410.00 | |||
Nek2 Double Nickase Plasmid (m2) | sc-421853-NIC-2 | 20 µg | $410.00 |
Nek2 (NIMA-related kinase 2) is a serine/threonine kinase that coordinates centrosome separation, bipolar spindle assembly, and faithful chromosome segregation during mitosis. In mouse cells, Nek2 activity integrates with cell-cycle and spindle checkpoint control by regulating centrosomal components and microtubule dynamics, thereby influencing progression through G2/M and mitotic fidelity. Dysregulated Nek2 expression or kinase activity is associated with centrosome amplification, aneuploidy, and genomic instability, processes that are frequently interrogated in models of proliferative and neurodevelopmental disorders. As a centrosome-associated kinase, Nek2 is commonly studied in pathways governing mitotic entry, spindle organization, and cilia-related cell-cycle coupling.
Nek2 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Nek2 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Nek2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Nek2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Nek2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.