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NEDD4-L Antibody (C-8) - Western Blotting - Image 357460 — NEDD4-L 抗体 (C-8): sc-514954. RAW 264. 7 (A) 和 NIH/3T3 (B) 全细胞裂解液中 NEDD4-L 表达的 Western 印迹分析.

NEDD4-L 抗体 (C-8): sc-514954. RAW 264. 7 (A) 和 NIH/3T3 (B) 全细胞裂解液中 NEDD4-L 表达的 Western 印迹分析.

NEDD4-L 抗体 (C-8): sc-514954

Name: NEDD4-L抗体 (C-8)
Brand: SCBT
SKU: sc-514954
Price: 316.0 USD
Availability: InStock

3.0(5)

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说明书

NEDD4-L 抗体 C-8 是小鼠单克隆 IgG₁ κ，NEDD4-L抗体, 在8篇文献中引用，规格为200 µg/ml
免疫原氨基酸序列213-350位于human物种的NEDD4-L的内部区域内
NEDD4-L 抗体 (C-8) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 NEDD4-L
抗NEDD4-L抗体（C-8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
还可偶联Alexa Fluor^® 488, Alexa Fluor^® 546, Alexa Fluor^® 594 和 Alexa Fluor^® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
还可偶联Alexa Fluor^® 680 和 Alexa Fluor^® 790，用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR^®/Odyssey^®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
关于如何获取NEDD4-L (C-8): sc-514954的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG₁ BP-HRP和m-IgGκ BP-HRP是NEDD4-L Antibody (C-8) 适用于 WB 应用。的首选辅助检测试剂。这些试剂现与NEDD4-L Antibody (C-8) 打包提供（请参阅下面的订购信息）。

查看产品引用文献(7)

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NEDD4-L 抗体 (C-8) 参考文献:

N4WBP5是Nedd4家族蛋白泛素化的潜在靶标，是一种新型的高尔基缔合作用蛋白。 | Harvey, KF., et al. 2002. J Biol Chem. 277: 9307-17. PMID: 11748237
位于人类染色体 18q21 上的 NEDD4L 有多种形式的转录本，是小鼠 Nedd4-2 基因的同源物。 | Chen, H., et al. 2001. Eur J Hum Genet. 9: 922-30. PMID: 11840194
雄激素对 LNCaP 人类前列腺癌细胞中 NEDD4L 转录物的表达具有不同的调节作用。 | Qi, H., et al. 2003. Mol Cell Endocrinol. 210: 51-62. PMID: 14615060
心脏电压门控钠通道 Nav1.5 受 Nedd4-2 介导的泛素化调控。 | van Bemmelen, MX., et al. 2004. Circ Res. 95: 284-91. PMID: 15217910
Nedd4-2与ClC-5的功能性相互作用：参与近端肾小管细胞的组成性白蛋白内吞。 | Hryciw, DH., et al. 2004. J Biol Chem. 279: 54996-5007. PMID: 15489223
14-3-3 蛋白通过与 Nedd4-2 泛素连接酶的磷酸化依赖性相互作用调节上皮 Na+ 通道的表达。 | Ichimura, T., et al. 2005. J Biol Chem. 280: 13187-94. PMID: 15677482
NEDD4L 的多态性与盐敏感性增加, P-renin 水平降低和 Nt-proANP 水平增加缔合作用。 | Dahlberg, J., et al. 2007. PLoS One. 2: e432. PMID: 17487281
Nedd4-2 催化细胞表面 ENaC 的泛素化和降解。 | Zhou, R., et al. 2007. J Biol Chem. 282: 20207-12. PMID: 17502380
人类 Nedd4L 基因变体的表达, 转录和可能的拮抗相互作用：对本质性高血压的影响。 | Araki, N., et al. 2008. Hypertension. 51: 773-7. PMID: 18268134
神经前体细胞表达发育下调8激活酶抑制剂MLN4924通过诱导DNA损伤、G2细胞周期阻滞和细胞凋亡，使结肠直肠癌细胞对奥沙利铂敏感。 | Zheng, W., et al. 2017. Mol Med Rep. 15: 2795-2801. PMID: 28447739
神经前体细胞表达的发育下调蛋白9在预测非肌肉浸润性膀胱癌对卡介苗反应中的作用。 | El-Babouly, IM., et al. 2018. Urol Oncol. 36: 242.e9-242.e14. PMID: 29519561
血清和糖皮质激素诱导激酶1通过神经前体细胞表达的发育下调蛋白4型2调节Hippo通路，从而调节多柔比星诱导的心肌细胞线粒体功能障碍和氧化应激。 | Zou, Z., et al. 2023. Hum Exp Toxicol. 42: 9603271231158039. PMID: 36781297

订购信息

产品名称	产品编号	规格	价格	数量	收藏夹
NEDD4-L 抗体 (C-8)	sc-514954	200 µg/ml	$316.00
NEDD4-L (C-8): m-IgG Fc BP-HRP 套装	sc-531251	200 µg Ab; 10 µg BP	$354.00
NEDD4-L (C-8): m-IgGκ BP-HRP 套装	sc-524884	200 µg Ab, 40 µg BP	$354.00
NEDD4-L (C-8): m-IgG₁ BP-HRP 套装	sc-544453	200 µg Ab; 20 µg BP	$354.00
NEDD4-L 抗体 (C-8) AC	sc-514954 AC	500 µg/ml, 25% agarose	$416.00
NEDD4-L 抗体 (C-8) HRP	sc-514954 HRP	200 µg/ml	$316.00
NEDD4-L 抗体 (C-8) FITC	sc-514954 FITC	200 µg/ml	$330.00
NEDD4-L 抗体 (C-8) PE	sc-514954 PE	200 µg/ml	$343.00
NEDD4-L 抗体 (C-8) Alexa Fluor^® 488	sc-514954 AF488	200 µg/ml	$357.00
NEDD4-L 抗体 (C-8) Alexa Fluor^® 546	sc-514954 AF546	200 µg/ml	$357.00
NEDD4-L 抗体 (C-8) Alexa Fluor^® 594	sc-514954 AF594	200 µg/ml	$357.00
NEDD4-L 抗体 (C-8) Alexa Fluor^® 647	sc-514954 AF647	200 µg/ml	$357.00
NEDD4-L 抗体 (C-8) Alexa Fluor^® 680	sc-514954 AF680	200 µg/ml	$357.00
NEDD4-L 抗体 (C-8) Alexa Fluor^® 790	sc-514954 AF790	200 µg/ml	$357.00

What is this about, I'm looking to help chronic kidney disease with Nedd4-2 enzyme. Does this sound familiar?

Asked by: Macc

Thank you for your question. For assistance please contact technical support by emailing scbt@scb.com or (800)-457-3801.

Answered by: BlakeJ

Date published: 2023-11-07

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with NEDD4-L (C-8): sc-514954?

Asked by: cjMara

We recommend using one of our our exclusive Mouse IgG Binding Proteins conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety

Answered by: Technical Support

Date published: 2017-02-27

Rated 1 out of 5 by Martina24 from Funziona molto debolmentePrimary antibody NEDD4-L (C-8) sc-514954 was used diluted 1:500 according to the manufacturer’s recommendations (for Western Blotting dilution range 1:100-1:1000), in Milk 5%. Membrane of PVDF has been incubated overnight at 4°C and then it has been probed for 1h at RT with Santa Cruz secondary antibodies diluted 1:5000 in Milk 1%. We have tested this antibody on two different sets of samples: in six cancer cell lines and in human activated PBMCs. Moreover, even if we tested the antibody at higher concentration, we only obtained a higher background but not an increase in the specific protein signal; therefore we show only the blots obtained after the incubation with antibodies prepared as described above. In conclusion: • NEDD4-L gave a weak signal, after a long exposure only in cancer samples but not in lymphocytes. Unfortunately we do not have other antibodies to be used for comparison, or other tools to modulate the levels of NEDD4-L in order to check the specificity. All the membranes have been probed with a normalizer and each gel has been stained with Coomassie Brillant Blue, shown on the right side of each panel, in order to demonstrate that the samples ran well and that the proteins are present on the SDS-PAGE.

Date published: 2019-11-26

Rated 3 out of 5 by DSSS from It is not very goodI got this free test ab two month ago, the first test looks good, but next time not very good, there were a lot nonspecific bands. And the size is different with website. I would like to try more.

Date published: 2018-09-21

Rated 3 out of 5 by ZHOU Jianwen from It is goodThe first I test this Antibody looks good, but next time, not very good, I would like to test more.

Date published: 2018-09-21

Rated 3 out of 5 by Little boy from A high backgroundI use this antibody to detet the protein in L929 WCL, with a dilution rate in1:500. I don't think the antibody can detet this protein very specifically. The upper strong intensity band my be the protein, but very differernt from the image in the website.

Date published: 2017-10-28

Rated 5 out of 5 by 小新贝贝 from Quite good for wb1:1000 diluted for wb of rat protein ,the band is special and strong.

Date published: 2017-08-28

NEDD4-L 抗体 (C-8) is rated 3.0 out of 5 by 5.

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种属	基因名称	基因ID	染色体位置	亚型（mRNA）登录号	蛋白质登录号	OMIM™ 号码
Human	NEDD4L	Entrez Gene ID: 23327	18q21.31	XM_005266658, XM_005266660, XM_005266663, XM_006722421, XM_006722424, XM_006722425, XM_006722426, XM_006722428, XM_006722430, XM_011525887, XM_017025676, XM_017025677, XM_017025678, XM_017025679, XM_017025680, XM_017025681, NM_001144964, NM_001144965, NM_001144966, NM_001144967, NM_001144968, NM_001144969, NM_001144970, NM_001144971, NM_001243960, NM_015277	UniProt ID: Q96PU5	606384
Mouse	Nedd4l	Entrez Gene ID: 83814	18 E1	XM_006526370, XM_006526371, XM_006526373, XM_006526374, XM_006526376, XM_006526377, XM_006526378, XM_006526379, XM_006526380, XM_006526382, XM_011247044, XM_011247047, XM_017318015, XM_017318016, NM_001114386, NM_031881	UniProt ID: Q8CFI0	N/A

NEDD4-L 抗体 (C-8): sc-514954

NEDD4-L 抗体 (C-8) 参考文献:

NEDD4-L 抗体 (C-8) 规格

替代产品名称: NEDD4-L

产品克隆名称: C-8

分类: 一抗

Clonality: 单克隆抗体

抗体同型: IgG₁ kappa light chain

氨基酸: 213-350

抗原表位靶标: NEDD4-L

抗原表位种属: human

产品反应性/检测: NEDD4-L

种属反应性/检测: mouse, rat, human

其它哺乳动物反应性: N/A

其它非哺乳动物反应性: N/A

应用: WB, IP, IF, ELISA

瓶的信息: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文: 8篇各种科学出版物的引文

NEDD4-L 抗体 (C-8)可选的偶联物/形式: AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

NEDD4-L 抗体 (C-8)

NEDD4-L (C-8): m-IgG Fc BP-HRP 套装

NEDD4-L (C-8): m-IgGκ BP-HRP 套装

NEDD4-L (C-8): m-IgG₁ BP-HRP 套装

NEDD4-L 抗体 (C-8) AC

NEDD4-L 抗体 (C-8) HRP

NEDD4-L 抗体 (C-8) FITC

NEDD4-L 抗体 (C-8) PE

NEDD4-L 抗体 (C-8) Alexa Fluor^® 488

NEDD4-L 抗体 (C-8) Alexa Fluor^® 546

NEDD4-L 抗体 (C-8) Alexa Fluor^® 594

NEDD4-L 抗体 (C-8) Alexa Fluor^® 647

NEDD4-L 抗体 (C-8) Alexa Fluor^® 680

NEDD4-L 抗体 (C-8) Alexa Fluor^® 790

What is this about, I'm looking to help chronic kidney disease with Nedd4-2 enzyme. Does this sound familiar?

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with NEDD4-L (C-8): sc-514954?

NEDD4-L 抗体 (C-8): sc-514954

NEDD4-L 抗体 (C-8) 参考文献:

NEDD4-L 抗体 (C-8) 规格

替代产品名称: NEDD4-L

产品克隆名称: C-8

分类: 一抗

Clonality: 单克隆抗体

抗体同型: IgG1 kappa light chain

氨基酸: 213-350

抗原表位靶标: NEDD4-L

抗原表位种属: human

产品反应性/检测: NEDD4-L

种属反应性/检测: mouse, rat, human

其它哺乳动物反应性: N/A

其它非哺乳动物反应性: N/A

应用: WB, IP, IF, ELISA

瓶的信息: Each vial contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文: 8篇各种科学出版物的引文

NEDD4-L 抗体 (C-8)可选的偶联物/形式: AC: Agarose, AF488: Alexa Fluor® 488, AF546: Alexa Fluor® 546, AF594: Alexa Fluor® 594, AF647: Alexa Fluor® 647, AF680: Alexa Fluor® 680, AF790: Alexa Fluor® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

NEDD4-L 抗体 (C-8)

NEDD4-L (C-8): m-IgG Fc BP-HRP 套装

NEDD4-L (C-8): m-IgGκ BP-HRP 套装

NEDD4-L (C-8): m-IgG1 BP-HRP 套装

NEDD4-L 抗体 (C-8) AC

NEDD4-L 抗体 (C-8) HRP

NEDD4-L 抗体 (C-8) FITC

NEDD4-L 抗体 (C-8) PE

NEDD4-L 抗体 (C-8) Alexa Fluor® 488

NEDD4-L 抗体 (C-8) Alexa Fluor® 546

NEDD4-L 抗体 (C-8) Alexa Fluor® 594

NEDD4-L 抗体 (C-8) Alexa Fluor® 647

NEDD4-L 抗体 (C-8) Alexa Fluor® 680

NEDD4-L 抗体 (C-8) Alexa Fluor® 790

What is this about, I'm looking to help chronic kidney disease with Nedd4-2 enzyme. Does this sound familiar?

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with NEDD4-L (C-8): sc-514954?

抗体同型: IgG₁ kappa light chain

瓶的信息: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

NEDD4-L 抗体 (C-8)可选的偶联物/形式: AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

NEDD4-L (C-8): m-IgG₁ BP-HRP 套装

NEDD4-L 抗体 (C-8) Alexa Fluor^® 488

NEDD4-L 抗体 (C-8) Alexa Fluor^® 546

NEDD4-L 抗体 (C-8) Alexa Fluor^® 594

NEDD4-L 抗体 (C-8) Alexa Fluor^® 647

NEDD4-L 抗体 (C-8) Alexa Fluor^® 680

NEDD4-L 抗体 (C-8) Alexa Fluor^® 790