Nanos3 CRISPR/Cas9 KO Plasmid (m): sc-434074
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Nanos3 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Nanos3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nanos3 CRISPR/Cas9 KO Plasmid (m) | sc-434074 | 20 µg | $397.00 |
Overview
Nanos3 encodes an evolutionarily conserved RNA-binding protein that helps maintain primordial germ cell survival and germline identity during mouse development. By associating with target mRNAs and modulating their stability and translational output, NANOS3 contributes to post-transcriptional gene regulation programs that coordinate germ cell migration, cell-cycle control, and suppression of inappropriate somatic differentiation. Disruption of Nanos3 is linked to germ cell depletion and infertility phenotypes, making it relevant to studies of reproductive biology and mechanisms that safeguard genome integrity in the germline. As a germline regulator, NANOS3 is also used as a marker and functional node for investigating developmental trajectories and stress responses in early gonadal lineages.
Nanos3 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Nanos3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Nanos3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Nanos3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Nanos3 protein expression.
This CRISPR knockout system enables efficient generation of Nanos3-deficient cell models for investigation of Nanos3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Nanos3 exon(s) critical for Nanos3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Nanos3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Nanos3 CRISPR/Cas9 KO Plasmid (m) and Nanos3 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Nanos3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Nanos3 HDR Plasmid (m) and Nanos3 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Nanos3 homology arms to support homology-directed repair at defined Nanos3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.