Date published: 2025-9-6
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N/R-cadherin Antibody (H-4) - Western Blotting - Image 350013
N/R-cadherin 抗体 (H-4): sc-271386. 人大脑皮层 (A) 和大鼠大脑 (B) 组织提取物中 N/R-cadherin 表达的 Western 印迹分析.
N/R-cadherin 抗体 (H-4): sc-271386. 人大脑皮层 (A) 和大鼠大脑 (B) 组织提取物中 N/R-cadherin 表达的 Western 印迹分析.

N/R-cadherin 抗体 (H-4): sc-271386

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说明书
  • N/R-cadherin抗体(H-4)是小鼠单克隆IgG2b κ, 在41篇文献中引用，规格为200 µg/ml
  • specific for an epitope mapping between amino acids 808-837 within a C-terminal cytoplasmic domain of N-cadherin of human origin
  • N/R-cadherin 抗体 (H-4) 推荐用于WB, IP, IF, IHC(P) 和 ELISA ，检测 mouse, rat, human 和 avian来源的N-cadherin and R-cadherin; 也和以下物种反应，包括： 和 equine, canine, bovine and avian
  • 抗N/R-cadherin抗体（H-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取N/R-cadherin (H-4): sc-271386的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 N/R-cadherin 抗体 (H-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与N/R-cadherin 抗体 (H-4) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Cadherin 抗体包括 cadherin-16, cadherin-19, cadherin-23, E-cadherin, EY-cadherin, K-cadherin, LI-cadherin, M-cadherin, N-cadherin 和 OB-cadherin

N/R-cadherin抗体（H-4）是一种IgG2b κ小鼠单克隆N/R-cadherin抗体，也被称为N/R-cadherin抗体或CDH2抗体，能够通过WB, IP, IF, IHC(P)和ELISA等方法检测小鼠、大鼠和人类来源的N-cadherin和R-cadherin。N/R-cadherin抗体（H-4）既可以作为非偶联抗N/R-cadherin抗体形式提供，也可以作为多种偶联形式的抗N/R-cadherin抗体提供，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®偶联物。钙粘蛋白是一类Ca2+依赖性的粘附分子，对维持组织结构和形态发生至关重要，通过介导细胞间的结合来实现其功能。经典的钙粘蛋白包括E-钙粘蛋白、N-钙粘蛋白和P-钙粘蛋白，这些蛋白质由大的细胞外结构域组成，特征是有一系列五个同源的NH2末端重复。这些钙粘蛋白中的最远端部分被认为负责结合特异性、跨膜结构域和羧基末端细胞内结构域。相对较短的细胞内结构域与多种细胞质蛋白（如β-连环蛋白）相互作用，从而调节钙粘蛋白的功能。粘附蛋白家族的成员还包括大鼠钙粘蛋白K（及其人类同源物钙粘蛋白-6）、R-钙粘蛋白、B-钙粘蛋白、E/P钙粘蛋白和钙粘蛋白-5等。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

N/R-cadherin 抗体 (H-4)

sc-271386
200 µg/ml
$316.00

N/R-cadherin (H-4): m-IgG Fc BP-HRP 套装

sc-529171
200 µg Ab; 10 µg BP
$354.00

N/R-cadherin (H-4): m-IgGκ BP-HRP 套装

sc-521926
200 µg Ab, 40 µg BP
$354.00

N/R-cadherin (H-4): m-IgG2b BP-HRP 套装

sc-550084
200 µg Ab; 10 µg BP
$354.00

N/R-cadherin 抗体 (H-4) AC

sc-271386 AC
500 µg/ml, 25% agarose
$416.00

N/R-cadherin 抗体 (H-4) HRP

sc-271386 HRP
200 µg/ml
$316.00

N/R-cadherin 抗体 (H-4) FITC

sc-271386 FITC
200 µg/ml
$330.00

N/R-cadherin 抗体 (H-4) PE

sc-271386 PE
200 µg/ml
$343.00

N/R-cadherin 抗体 (H-4) Alexa Fluor® 488

sc-271386 AF488
200 µg/ml
$357.00

N/R-cadherin 抗体 (H-4) Alexa Fluor® 546

sc-271386 AF546
200 µg/ml
$357.00

N/R-cadherin 抗体 (H-4) Alexa Fluor® 594

sc-271386 AF594
200 µg/ml
$357.00

N/R-cadherin 抗体 (H-4) Alexa Fluor® 647

sc-271386 AF647
200 µg/ml
$357.00

N/R-cadherin 抗体 (H-4) Alexa Fluor® 680

sc-271386 AF680
200 µg/ml
$357.00

N/R-cadherin 抗体 (H-4) Alexa Fluor® 790

sc-271386 AF790
200 µg/ml
$357.00

N/R-cadherin (H-4) 中和勝肽

sc-271386 P
100 µg/0.5 ml
$68.00

This antibody is suggested as a replacement for the discontinued: Sc-7939. It does not work for IF in our system, while Sc-7939 worked extremely well. Do you have another suggested replacement for Sc-7939 for IF protocols?

Asked by: Morgani_S
Thank you for your question. Please contact our Technical Service Department or your local distributor for assistance in troubleshooting this antibody.
Answered by: Tech Service 1
Date published: 2021-01-21

Can the peptide sc-271386 P be used in Western Blot as a positive control?

Asked by: AbPolly
Thank you for your question. The blocking peptide sc-271386 P is a short synthetic peptide intended for use as a negative control by preadsorption. It is not appropriate for use as a positive control in Western Blot.
Answered by: Technical Service
Date published: 2017-02-02
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Rated 1 out of 5 by from Did not work for IFI ordered this antibody as it was a recommended replacement for Sc-7939, which was discontinued. We routinely used Sc-7939 for IF staining on early mouse embryos and it worked wonderfully. This antibody does not work for IF in the same system and hence cannot be used as a replacement in our case.
Date published: 2020-07-27
Rated 1 out of 5 by from Didnot work for WBdid not work for western - samples were human cells in culture
Date published: 2017-11-21
Rated 5 out of 5 by from goodThis is antibody is specific great antibody for Western blotting.
Date published: 2017-09-07
Rated 5 out of 5 by from Excellent antibodyThe bond is single, clear, and the background is low. Compared with other antibody, it is stable and effective.
Date published: 2017-09-06
Rated 3 out of 5 by from single band but not very shapI used this antibody in the ratio 1:1000 in pancreatic cancer cell line and I got a single band but it was not very shap.
Date published: 2017-08-30
Rated 4 out of 5 by from Good antibodyI used this antibody in a dilution 1:1000, in most of the case, a clear band can be acquired.
Date published: 2017-08-29
Rated 3 out of 5 by from Function in generalI tried the product and found the result was not strong by western.
Date published: 2017-08-24
Rated 1 out of 5 by from trashinessdilution ratio1:300 in WB for lovo,sw480,sw48,hct116 and other CRC. 2 days of incubation can get nothing!!!
Date published: 2017-08-22
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N/R-cadherin 抗体 (H-4) is rated 4.0 out of 5 by 29.
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