Date published: 2025-9-7
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MYH11 Antibody (G-4) - Immunofluorescence - Image 5423
MYH11 抗体 (G-4): sc-6956. 甲醇固定的 NIH/3T3 细胞免疫荧光染色, 显示肌球蛋白重链的细胞骨架罗丹明免疫染色. 另请注意核罗丹明免疫染色与 Cdk4 (C-22): sc-260.
MYH11 抗体 (G-4): sc-6956. 甲醇固定的 NIH/3T3 细胞免疫荧光染色, 显示肌球蛋白重链的细胞骨架罗丹明免疫染色. 另请注意核罗丹明免疫染色与 Cdk4 (C-22): sc-260.

MYH11 抗体 (G-4): sc-6956

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说明书
  • MYH11抗体(G-4)是小鼠单克隆IgG1 λ MYH11抗体, 在72篇文献中引用，规格为200 µg/ml
  • 针对全长平滑肌肌球蛋白重链（rat来源）
  • MYH11 抗体 (G-4) 推荐用于 WB, IP, IF, FCM 和 ELISA，检测mouse, rat 和human 来源的 myosin heavy chain 11, isoforms SM1 and SM2; 也和以下物种反应，包括： 和 bovine and porcine
  • 抗MYH11抗体（G-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取MYH11 (G-4): sc-6956的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是MYH11 Antibody (G-4) for WB applications. 的首选辅助检测试剂，这些试剂现与MYH11 Antibody (G-4) 打包提供（请参阅下面的订购信息）。

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相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Myosin抗体，包括MRLC2, MYH, MYH1, MYH10, MYH11, MYH15, MYH2, MYH3, MYH4, MYH6, MYH7, MYH9, MYL, MYL1, MYL2和MYL3

MYH11抗体（G-4）是一种小鼠单克隆IgG1抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、流式细胞术（FCM）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人源MYH11蛋白。抗MYH11抗体（G-4）针对大鼠来源的全长平滑肌肌球蛋白重链。MYH11 (G-4) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。MYH11蛋白，也称为平滑肌肌球蛋白重链，通过与肌动蛋白丝相互作用产生力量，在肌肉收缩和细胞运动中起着至关重要的作用。这种相互作用对于各种生理过程至关重要，包括血管收缩和胃肠蠕动，这使得MYH11成为研究平滑肌功能和相关疾病的重要靶标。MYH11的结构由六聚体组装而成，包括两个可被磷酸化或不可被磷化的重链亚基和轻链亚基。肌球蛋白轻链的磷酸化状态是平滑肌收缩的关键调节机制，由钙/钙调蛋白依赖性肌球蛋白轻链激酶调节。了解MYH11的结构和功能对于阐明平滑肌生理和病理生理机制以及开发平滑肌功能障碍的治疗策略至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MYH11 抗体 (G-4) 参考文献:

  1. 骨骼肌发育过程中肌球蛋白重链基因表达的时空变化  |  Lu, BD., et al. 1999. Dev Biol. 216: 312-26. PMID: 10588881
  2. 鉴定人类肌球蛋白重链的三种发育受控异构体  |  Bober, E., et al. 1990. Eur J Biochem. 189: 55-65. PMID: 1691980
  3. 生成全长的人类围产期肌球蛋白重链编码 cDNA。  |  Karsch-Mizrachi, I., et al. 1990. Gene. 89: 289-94. PMID: 2373371
  4. 通过 cDNA 克隆和免疫印迹分析鉴定两种平滑肌肌球蛋白重链同工酶。  |  Nagai, R., et al. 1989. J Biol Chem. 264: 9734-7. PMID: 2722872
  5. Myh11系角膜内皮细胞和ASCs填充角膜内皮细胞  |  Corliss, BA., et al. 2019. Invest Ophthalmol Vis Sci. 60: 5095-5103. PMID: 31826231
  6. RUNX1-RUNX1T1和CBFB-MYH11对急性髓性白血病KIT突变预后影响的前瞻性评估  |  Ishikawa, Y., et al. 2020. Blood Adv. 4: 66-75. PMID: 31899799
  7. 产生内源性 Myh11 驱动的 Cre 小鼠，在平滑肌细胞中实现性别无关的基因缺失。  |  Zhao, Y., et al. 2023. JCI Insight. 8: PMID: 37289544
  8. 人类围产期肌球蛋白重链转录本的特征。  |  Jullian, EH., et al. 1995. Eur J Biochem. 230: 1001-6. PMID: 7601129
  9. 调节血管平滑肌细胞的分化。  |  Owens, GK. 1995. Physiol Rev. 75: 487-517. PMID: 7624392
  10. 肌球蛋白超家族的系统发育分析。  |  Cheney, RE., et al. 1993. Cell Motil Cytoskeleton. 24: 215-23. PMID: 8477454
  11. 平滑肌收缩机制  |  Horowitz, A., et al. 1996. Physiol Rev. 76: 967-1003. PMID: 8874491
  12. 哺乳动物肌球蛋白重链基因家族。  |  Weiss, A. and Leinwand, LA. 1996. Annu Rev Cell Dev Biol. 12: 417-39. PMID: 8970733

订购信息

产品名称产品编号规格价格数量收藏夹

MYH11 抗体 (G-4)

sc-6956
200 µg/ml
$316.00

MYH11 (G-4): m-IgG Fc BP-HRP 套装

sc-526487
200 µg Ab; 10 µg BP
$354.00

MYH11 (G-4): m-IgG1 BP-HRP 套装

sc-531860
200 µg Ab; 20 µg BP
$354.00

MYH11 抗体 (G-4) AC

sc-6956 AC
500 µg/ml, 25% agarose
$416.00

MYH11 抗体 (G-4) HRP

sc-6956 HRP
200 µg/ml
$316.00

MYH11 抗体 (G-4) FITC

sc-6956 FITC
200 µg/ml
$330.00

MYH11 抗体 (G-4) PE

sc-6956 PE
200 µg/ml
$343.00

MYH11 抗体 (G-4) Alexa Fluor® 488

sc-6956 AF488
200 µg/ml
$357.00

MYH11 抗体 (G-4) Alexa Fluor® 546

sc-6956 AF546
200 µg/ml
$357.00

MYH11 抗体 (G-4) Alexa Fluor® 594

sc-6956 AF594
200 µg/ml
$357.00

MYH11 抗体 (G-4) Alexa Fluor® 647

sc-6956 AF647
200 µg/ml
$357.00

MYH11 抗体 (G-4) Alexa Fluor® 680

sc-6956 AF680
200 µg/ml
$357.00

MYH11 抗体 (G-4) Alexa Fluor® 790

sc-6956 AF790
200 µg/ml
$357.00

Can I have a small amount of this antibody for testing please? MYH11 Antibody (G-4) HRP (sc-6956 HRP). My shipping address: Room 1-301, Building 710, 3801 Miranda Ave, Palo Alto VA Medical Center, Palo Alto, CA 94304. Phone: 6507134776.

Asked by: Baohui Xu
Thank you for your question. We do not provide conjugated antibody samples . To get more information about our sample program please call (800-457-3801 or email scbt@scbt.com
Answered by: BlakeJ
Date published: 2024-08-20

Hello, I am about to try the anti MYH11 antibody sc-6956, but I am a little confused from the picture in datasheet. There is a fibroblast NIH/3T3, which should be MYH11 negative. Is this antibody really specific for muscle cells myosin? Thanks.

Asked by: Jarmila
Thank you for your question. We are looking into any references we can provide to support the data in NIH/3T3 using this antibody, and will update the information on our website as soon as we can provide more information. In the meantime, we are currently offering a free 10 µg sample of this antibody, MYH11 (G-4): sc-6956, if you would be interested in testing the antibody in your samples. Please contact our Technical Service Department if you would like to request a sample.
Answered by: Technical Support
Date published: 2022-11-10

MYH11 (G-4): sc-6956 monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: jenni
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. SH-PTP2 (B-1): sc-7384 monoclonal antibody is currently available conjugated to PE, FITC, Alexa Fluor 488 or Alexa Fluor 647.
Answered by: Technical Support 10
Date published: 2017-01-09

Hello, can you please tell me whether this Ab recognizes zebrafish myh11 (at least whether it could possibly recognize this based on sequence similarity?) Thanks.

Asked by: Calgaryfish
Thank you for your inquiry. Unfortunately we do not have any information regarding zebrafish reactivity for this MYH11 antibody and can not warranty it for that use. Please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Tech Service
Date published: 2016-10-24
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Rated 5 out of 5 by from mouse anti-MYH11 works well for ICCmouse anti-MYH11 works well for ICC on murine brown adipocytes.
Date published: 2023-09-22
Rated 5 out of 5 by from IHC-Fr is goodit was good for IHC-Fr , the dilution ratio was 1:100.
Date published: 2018-07-07
Rated 5 out of 5 by from great productWe used this antibody for immunostaining. it works for our experiments.
Date published: 2017-09-27
Rated 5 out of 5 by from nice antibodyI used this MYH11 as 1:200 for IF staining, it was pretty good, yet the confocal results were put into our paper, thanks for SANTA.
Date published: 2017-09-14
Rated 1 out of 5 by from Not working in Gastric cancer cell linesNo any specific WB band detected in gastric cancer cell lines.
Date published: 2017-06-21
Rated 5 out of 5 by from Perform a perfect deductionIn order to test for contractile activity in smooth muscle, we purchased myosin, which is highly conserved and we perform a perfect deduction experiment by smooth muscle contracting on myosin light chain kinase-dependent regulation.
Date published: 2017-02-10
Rated 4 out of 5 by from Good performance in Western blotThis antibody was used in WB with a 1:500 dilution, and a specific band was detected in the membrane.
Date published: 2017-01-06
Rated 5 out of 5 by from Excellent antibody.This is a great product that works well in standard applications. It has broad reactivity and will produce clear and specific signals. It has been published with over twenty times.
Date published: 2017-01-04
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MYH11 抗体 (G-4) is rated 4.6 out of 5 by 14.
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