Date published: 2025-9-8
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MSH2 Antibody (D-6) - Western Blotting - Image 286123
MSH2 抗体 (D-6): sc-376384. Hep G2 (A), Caki-1 (B) 和 EOC 20 (C) 全细胞裂解液中 MSH2 表达的 Western 印迹分析.
MSH2 抗体 (D-6): sc-376384. Hep G2 (A), Caki-1 (B) 和 EOC 20 (C) 全细胞裂解液中 MSH2 表达的 Western 印迹分析.

MSH2 抗体 (D-6): sc-376384

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寫評論提問

说明书
  • MSH2 抗体 D-6 是小鼠单克隆 IgG1 κ，MSH2抗体, 在5篇文献中引用，规格为200 µg/ml
  • 免疫human物种的MSH2的氨基酸1-300
  • MSH2 抗体 (D-6) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 MSH2
  • 抗MSH2抗体（D-6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取MSH2 (D-6): sc-376384的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP是MSH2 Antibody (D-6) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现可与MSH2 Antibody (D-6) 搭配使用（请参阅下面的订购信息）。有关其它 m-IgGκ BP 结合物，请参阅我们完整的小鼠 IgG 结合蛋白列表。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

额外MSH抗体，包括MSH3

MSH2抗体（D-6）是一种IgG1 κ小鼠单克隆MSH2抗体（也称为MutS同源物2抗体、DNA错配修复蛋白Msh2抗体、HNPCC1抗体、HNPCC抗体、MutS（大肠杆菌）同源物2（结肠癌非息肉病类型1）抗体、MutS同源物2结肠癌非息肉病类型1（大肠杆菌）抗体、MutS同源物2结肠癌非息肉病抗体、MutS蛋白同源物2抗体、COCA1抗体、LCFS2抗体或FCC1抗体），可通过WB、IP、IF和ELISA方法检测小鼠、大鼠和人类来源的MSH2蛋白。MSH2抗体（D-6）既有非偶联形式的抗MSH2抗体，也有多种偶联形式的抗MSH2抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。发现DNA错配修复基因的突变与遗传性非息肉病性结直肠癌（HNPCC）有关后，人们对DNA错配修复机制的理解产生了浓厚的兴趣。最初，在HNPCC中高频检测到细菌DNA错配修复基因MutS和MutL的MSH2和MLH1同源物的遗传性突变，并显示这些突变与微卫星不稳定性有关。10%至45%的胰腺癌、胃癌、乳腺癌、卵巢癌和小细胞肺癌也表现出微卫星不稳定性，这表明DNA错配修复不仅限于HNPCC肿瘤，而是肿瘤发生或进展的一个共同特征。已经确定了原核MutL基因的两个额外同源物，分别命名为PMS1和PMS2，并显示它们在HNPCC患者的生殖系中发生突变。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MSH2 抗体 (D-6) 参考文献:

  1. HDAC6脱乙酰化和泛素化MSH2以维持适当的MutSα水平。  |  Zhang, M., et al. 2014. Mol Cell. 55: 31-46. PMID: 24882211
  2. MSH2失调由前炎细胞因子刺激触发并与肝癌发展有关  |  Eso, Y., et al. 2016. Cancer Res. 76: 4383-93. PMID: 27261510
  3. MLH1/MSH2对II/III期散发性结直肠癌的临床意义。  |  Wang, SM., et al. 2019. World J Gastrointest Oncol. 11: 1065-1080. PMID: 31798786
  4. MSH2中SVA元素的插入是林奇综合征的新病因。  |  Yang, C., et al. 2021. Genes Chromosomes Cancer. 60: 571-576. PMID: 33822432
  5. 疑似林奇综合征的大肠癌患者中，细胞质MSH2与MSH2/EPCAM基因中的基因组缺失有关。  |  Dong, L., et al. 2021. Front Oncol. 11: 627460. PMID: 34055602
  6. 基于生物信息学的MSH2在泛癌中的表达和预后价值分析。  |  Qiu, W., et al. 2021. Biomed Res Int. 2021: 9485273. PMID: 34859104
  7. Andrias davidianus Ranavirus (ADRV) 基因组通过结合细胞错配修复蛋白MSH2高效复制。  |  Ke, F., et al. 2022. Viruses. 14: PMID: 35632694
  8. 共济失调毛细血管扩张突变和MSH2控制Ig类转换重组中的钝DNA末端连接。  |  Sible, E., et al. 2023. J Immunol. 210: 369-376. PMID: 36603026
  9. 蛋白质谱显示MSH6/MSH2是肝细胞癌微血管浸润的潜在生物标志物。  |  Hong, S., et al. 2024. Hepatol Res. 54: 189-200. PMID: 37776019
  10. MSH2基因中存在新突变的Muir-Torre综合征。  |  Ustaoglu, E., et al. 2023. Acta Dermatovenerol Croat. 31: 144-147. PMID: 38439724

订购信息

产品名称产品编号规格价格数量收藏夹

MSH2 抗体 (D-6)

sc-376384
200 µg/ml
$316.00

MSH2 (D-6): m-IgGκ BP-HRP 套装

sc-522938
200 µg Ab, 40 µg BP
$354.00

MSH2 抗体 (D-6) AC

sc-376384 AC
500 µg/ml, 25% agarose
$416.00

MSH2 抗体 (D-6) HRP

sc-376384 HRP
200 µg/ml
$316.00

MSH2 抗体 (D-6) FITC

sc-376384 FITC
200 µg/ml
$330.00

MSH2 抗体 (D-6) PE

sc-376384 PE
200 µg/ml
$343.00

MSH2 抗体 (D-6) Alexa Fluor® 488

sc-376384 AF488
200 µg/ml
$357.00

MSH2 抗体 (D-6) Alexa Fluor® 546

sc-376384 AF546
200 µg/ml
$357.00

MSH2 抗体 (D-6) Alexa Fluor® 594

sc-376384 AF594
200 µg/ml
$357.00

MSH2 抗体 (D-6) Alexa Fluor® 647

sc-376384 AF647
200 µg/ml
$357.00

MSH2 抗体 (D-6) Alexa Fluor® 680

sc-376384 AF680
200 µg/ml
$357.00

MSH2 抗体 (D-6) Alexa Fluor® 790

sc-376384 AF790
200 µg/ml
$357.00

I know D-6 is the replacement of N-20. Could you tell me does they have the same immunogen amino acid sequences? Their applications look like the same.

Asked by: JamesChou
Thank you for your question. The two antibodies have overlapping immunogen sequences, but they are not the same. This new mouse monoclonal antibody, MSH2 (D-6): sc-376384, is raised against a recombinant protein corresponding to amino acids 1-300 of MSH2 of human origin. The rabbit polyclonal antibody, MSH2 (N-20): sc-494, was raised against a short peptide sequence mapping at the N-terminus of MSH2 of human origin. We do offer a different monoclonal antibody, MSH2 (E-7): sc-365052, which is raised against the same peptide used to generate the rabbit polyclonal (N-20) antibody.
Answered by: Technical Support
Date published: 2017-10-05

What is the recommended fixation method for immunofluorescence staining with MSH2 (D-6): sc-376384 monoclonal antibody?

Asked by: Germaine
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from good for WBWe used this antibody in U2OS and E14 cells. It is very strong and clear.
Date published: 2018-02-20
Rated 4 out of 5 by from Works great for Western BlottingI ordered a free sample to test this one out few weeks ago in human corneal endothelial cells. It gave a strong signal when used at 1:500 dilution in 5% milk+ TBS-tween and developed with mouse-IgGκ BP-HRP(SCBT) at 1:1000 dilution in 5% milk+ TBS-tween.
Date published: 2018-02-02
Rated 5 out of 5 by from Worked in Western blotworks well in Western blot of Human Hek293 cells. Block: 5%milk PBST primary antibody: 1/500 in 1% milk PBST cold room overnight secondary antibody: 1/10,000 anti-mouse HRP in 1% milk PBST ECL Dura substrate 5 minutes
Date published: 2017-11-29
Rated 5 out of 5 by from Good antibodyI requested this free antibody sample a few weeks ago, it works in my Hela samples at 1:100 dilution, blocked by 5% non-fat milk, but has some other bands below
Date published: 2017-10-03
Rated 5 out of 5 by from good for WBI used this antiboty 1:1000 for WB, the band is clear and have no background
Date published: 2017-09-23
Rated 3 out of 5 by from okThis antibody worked well for my WB by using human keratinocyte.
Date published: 2017-02-09
Rated 5 out of 5 by from Versatile Antibody, Strong Signal in WBThis antibody is well-cited for a variety of applications including WB, IP, IF, and even ELISA. Strong, clear signal with little to no background. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-02-03
Rated 5 out of 5 by from Highly potent and very specific antibodyThis monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was very strong detection of the ~100 kDa target protein in an A431/HepG2/MCF7 cell lysate mix, and weak detection in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested or in the A431/HepG2/MCF7 cell lysate mix.
Date published: 2016-11-10
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MSH2 抗体 (D-6) is rated 4.7 out of 5 by 11.
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